High-Throughput DNA Metabarcoding as an Approach for Ichthyoplankton Survey in Oujiang River Estuary, China
High-throughput DNA metabarcoding of mitochondrial 12S rRNA and Cyt b gene sequences was coupled with a morphology-based identification tool to assess ichthyoplankton community structure in Oujiang River Estuary, China. The performances of 12S and Cyt b barcoding markers were compared in terms of ta...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Diversity |
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| Online Access: | https://www.mdpi.com/1424-2818/14/12/1111 |
| _version_ | 1797460547059318784 |
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| author | Rijin Jiang James Leonard Lusana Yongjiu Chen |
| author_facet | Rijin Jiang James Leonard Lusana Yongjiu Chen |
| author_sort | Rijin Jiang |
| collection | DOAJ |
| description | High-throughput DNA metabarcoding of mitochondrial 12S rRNA and Cyt b gene sequences was coupled with a morphology-based identification tool to assess ichthyoplankton community structure in Oujiang River Estuary, China. The performances of 12S and Cyt b barcoding markers were compared in terms of taxonomic resolution, detection and coverage, and their suitability was established for use as a quick and powerful ichthyoplankton assessment tool. A total of 30,138 ichthyoplankton (2462 eggs and 27,676 larvae) samples were collected from April to August 2015 and identified to 145 taxa belonging to 57 families and 105 genera. June and July were the main spawning months. Ichthyoplankton were more abundant around Lingkun and Qidu Islands and the upper parts of Oujiang River Estuary. The 12S gene marker presented higher species coverage and detection rate than Cyt b. DNA metabarcoding exhibited more representative species identification power than morphology. The findings reported in this study provided a key attempt towards the development of time-efficient and cost-effective ichthyoplankton identification and assessment tool. |
| first_indexed | 2024-03-09T17:06:35Z |
| format | Article |
| id | doaj.art-9bebe0da4a4a4125bdc0be0b8e8c0c33 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-09T17:06:35Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-9bebe0da4a4a4125bdc0be0b8e8c0c332023-11-24T14:23:48ZengMDPI AGDiversity1424-28182022-12-011412111110.3390/d14121111High-Throughput DNA Metabarcoding as an Approach for Ichthyoplankton Survey in Oujiang River Estuary, ChinaRijin Jiang0James Leonard Lusana1Yongjiu Chen2Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, ChinaCollege of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaCollege of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaHigh-throughput DNA metabarcoding of mitochondrial 12S rRNA and Cyt b gene sequences was coupled with a morphology-based identification tool to assess ichthyoplankton community structure in Oujiang River Estuary, China. The performances of 12S and Cyt b barcoding markers were compared in terms of taxonomic resolution, detection and coverage, and their suitability was established for use as a quick and powerful ichthyoplankton assessment tool. A total of 30,138 ichthyoplankton (2462 eggs and 27,676 larvae) samples were collected from April to August 2015 and identified to 145 taxa belonging to 57 families and 105 genera. June and July were the main spawning months. Ichthyoplankton were more abundant around Lingkun and Qidu Islands and the upper parts of Oujiang River Estuary. The 12S gene marker presented higher species coverage and detection rate than Cyt b. DNA metabarcoding exhibited more representative species identification power than morphology. The findings reported in this study provided a key attempt towards the development of time-efficient and cost-effective ichthyoplankton identification and assessment tool.https://www.mdpi.com/1424-2818/14/12/1111ichthyoplanktonOujiang River Estuarymetabarcodingmorphology12SCyt b |
| spellingShingle | Rijin Jiang James Leonard Lusana Yongjiu Chen High-Throughput DNA Metabarcoding as an Approach for Ichthyoplankton Survey in Oujiang River Estuary, China Diversity ichthyoplankton Oujiang River Estuary metabarcoding morphology 12S Cyt b |
| title | High-Throughput DNA Metabarcoding as an Approach for Ichthyoplankton Survey in Oujiang River Estuary, China |
| title_full | High-Throughput DNA Metabarcoding as an Approach for Ichthyoplankton Survey in Oujiang River Estuary, China |
| title_fullStr | High-Throughput DNA Metabarcoding as an Approach for Ichthyoplankton Survey in Oujiang River Estuary, China |
| title_full_unstemmed | High-Throughput DNA Metabarcoding as an Approach for Ichthyoplankton Survey in Oujiang River Estuary, China |
| title_short | High-Throughput DNA Metabarcoding as an Approach for Ichthyoplankton Survey in Oujiang River Estuary, China |
| title_sort | high throughput dna metabarcoding as an approach for ichthyoplankton survey in oujiang river estuary china |
| topic | ichthyoplankton Oujiang River Estuary metabarcoding morphology 12S Cyt b |
| url | https://www.mdpi.com/1424-2818/14/12/1111 |
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