Plaunotol from Croton stellatopilosus Ohba inhibited cell growth and induced apoptosis in human cancer cell lines
Plaunotol is an acyclic diterpene alcohol that was evaluated for anti-proliferative activity in four human cancer cell lines: HeLa; HT-29; MCF-7; and KB. After the cells were treated with plaunotol, cell viability was determined using the MTT assay. The results showed that plaunotol inhibited the...
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Prince of Songkla University
2019-08-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
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| Online Access: | https://rdo.psu.ac.th/sjstweb/journal/41-4/18.pdf |
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| author | Charoenwong Premprasert Supreeya Yoenyongsawad Supinya Tewtrakul Juraithip Wungsintaweekul |
| author_facet | Charoenwong Premprasert Supreeya Yoenyongsawad Supinya Tewtrakul Juraithip Wungsintaweekul |
| author_sort | Charoenwong Premprasert |
| collection | DOAJ |
| description | Plaunotol is an acyclic diterpene alcohol that was evaluated for anti-proliferative activity in four human cancer cell
lines: HeLa; HT-29; MCF-7; and KB. After the cells were treated with plaunotol, cell viability was determined using the MTT
assay. The results showed that plaunotol inhibited the growth of human cancer cell lines with an IC50 of 65.47±6.39 μM (HeLa),
72.92±5.73 μM (HT-29), 80.90±3.48 μM (KB), and 62.25±9.15 μM (MCF-7). For apoptotic detection, the cells treated with
plaunotol were stained with Annexin-V/7-AAD reagent. The results indicated that plaunotol induced cell death or apoptosis. The
transcription profile of apoptotic-associated genes, including TNF-α, BCL-2, BAX, and BAK genes, was determined using the
qRT-PCR technique and the expression level was calculated as the relative quantitation (RQ). The ratio between RQ of BCL-2
and BAX suggested that plaunotol significantly induced apoptosis in HeLa, MCF-7, and HT-29 cell lines, in particular. |
| first_indexed | 2024-12-17T06:10:31Z |
| format | Article |
| id | doaj.art-9bec7f1902584228a41694787d769c28 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-17T06:10:31Z |
| publishDate | 2019-08-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-9bec7f1902584228a41694787d769c282022-12-21T22:00:39ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952019-08-0141484685510.14456/sjst-psu.2019.108Plaunotol from Croton stellatopilosus Ohba inhibited cell growth and induced apoptosis in human cancer cell linesCharoenwong Premprasert0Supreeya Yoenyongsawad1Supinya Tewtrakul2Juraithip Wungsintaweekul3Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112 ThailandDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112 ThailandDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112 ThailandDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112 ThailandPlaunotol is an acyclic diterpene alcohol that was evaluated for anti-proliferative activity in four human cancer cell lines: HeLa; HT-29; MCF-7; and KB. After the cells were treated with plaunotol, cell viability was determined using the MTT assay. The results showed that plaunotol inhibited the growth of human cancer cell lines with an IC50 of 65.47±6.39 μM (HeLa), 72.92±5.73 μM (HT-29), 80.90±3.48 μM (KB), and 62.25±9.15 μM (MCF-7). For apoptotic detection, the cells treated with plaunotol were stained with Annexin-V/7-AAD reagent. The results indicated that plaunotol induced cell death or apoptosis. The transcription profile of apoptotic-associated genes, including TNF-α, BCL-2, BAX, and BAK genes, was determined using the qRT-PCR technique and the expression level was calculated as the relative quantitation (RQ). The ratio between RQ of BCL-2 and BAX suggested that plaunotol significantly induced apoptosis in HeLa, MCF-7, and HT-29 cell lines, in particular.https://rdo.psu.ac.th/sjstweb/journal/41-4/18.pdfplaunotolanti-proliferative activityMTT assayapoptosishuman cancer cell lines |
| spellingShingle | Charoenwong Premprasert Supreeya Yoenyongsawad Supinya Tewtrakul Juraithip Wungsintaweekul Plaunotol from Croton stellatopilosus Ohba inhibited cell growth and induced apoptosis in human cancer cell lines Songklanakarin Journal of Science and Technology (SJST) plaunotol anti-proliferative activity MTT assay apoptosis human cancer cell lines |
| title | Plaunotol from Croton stellatopilosus Ohba inhibited cell growth and induced apoptosis in human cancer cell lines |
| title_full | Plaunotol from Croton stellatopilosus Ohba inhibited cell growth and induced apoptosis in human cancer cell lines |
| title_fullStr | Plaunotol from Croton stellatopilosus Ohba inhibited cell growth and induced apoptosis in human cancer cell lines |
| title_full_unstemmed | Plaunotol from Croton stellatopilosus Ohba inhibited cell growth and induced apoptosis in human cancer cell lines |
| title_short | Plaunotol from Croton stellatopilosus Ohba inhibited cell growth and induced apoptosis in human cancer cell lines |
| title_sort | plaunotol from croton stellatopilosus ohba inhibited cell growth and induced apoptosis in human cancer cell lines |
| topic | plaunotol anti-proliferative activity MTT assay apoptosis human cancer cell lines |
| url | https://rdo.psu.ac.th/sjstweb/journal/41-4/18.pdf |
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