Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium
Background: Bone marrow mesenchymal stem cells (MSCs) are one of the undifferentiated multipotential cell sources of human body. Methods: MSCs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. Learning about responses of MSCs to external milieu and chemical f...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2014-12-01
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| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
| Subjects: | |
| Online Access: | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/408 |
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| author | Farshad Homayouni Moghadam Tahereh Tayebi Maryam Dehghan Gilda Eslami Hamid Nadri Alireza Moradi Hassanali Vahedian-Ardakani Kazem Barzegar |
| author_facet | Farshad Homayouni Moghadam Tahereh Tayebi Maryam Dehghan Gilda Eslami Hamid Nadri Alireza Moradi Hassanali Vahedian-Ardakani Kazem Barzegar |
| author_sort | Farshad Homayouni Moghadam |
| collection | DOAJ |
| description | Background: Bone marrow mesenchymal stem cells (MSCs) are one of the undifferentiated multipotential cell sources of human body.
Methods: MSCs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. Learning about responses of MSCs to external milieu and chemical factors such as pH could recommend new approaches for preparation of suitable scaffolds for bone and cartilage tissue engineering. In present study, the effect of alkaline medium on chondrogenic and osteogenic differentiation of rat MSCs was evaluated.MSCs were harvested from bone marrow of animals and then the response of passage1 and 2 of MSCs (P1 MSCs & P2 MSCs) to the culture in alkaline medium (pH: 8) was evaluated. Cytochemical and immunocytochemical staining were performed to distinguish chondrocytes and osteocytes. Real-time PCR was performed to evaluate the type II collagen and osteopontin mRNA levels.
Results: Staining for type II collagen, a chondrocytic specific marker, revealed that after one-week culture in alkaline medium, a considerable amount of P1 MSCs had shown chondrocytic morphology. By prolonging the culture period up to 4 weeks, osteogenic cells with expanded matrix and mineralized areas around them were appeared. Results of real-time PCR showed that P1 MSCs after one week culture in alkaline medium expressed highest rate of type II collagen and osteopontin mRNA among all groups.
Conclusion: This study demonstrated that alkaline medium is a potent chondrogenic differentiation inducer for MSCs in their first passage. |
| first_indexed | 2024-03-12T09:02:51Z |
| format | Article |
| id | doaj.art-9bee0ddd977046459dd1903d85a080c5 |
| institution | Directory Open Access Journal |
| issn | 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T09:02:51Z |
| publishDate | 2014-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-9bee0ddd977046459dd1903d85a080c52023-09-02T15:34:00ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-22072014-12-0184394Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline MediumFarshad Homayouni Moghadam0Tahereh Tayebi1Maryam Dehghan2Gilda Eslami3Hamid Nadri4Alireza Moradi5Hassanali Vahedian-Ardakani6Kazem Barzegar7Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. AND Neurobiomedical Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Neurobiomedical Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Department of Medicinal Chemistry, School of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Department of Medicinal Chemistry, School of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Department of Internal Medicine, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.English Language Department, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Background: Bone marrow mesenchymal stem cells (MSCs) are one of the undifferentiated multipotential cell sources of human body. Methods: MSCs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. Learning about responses of MSCs to external milieu and chemical factors such as pH could recommend new approaches for preparation of suitable scaffolds for bone and cartilage tissue engineering. In present study, the effect of alkaline medium on chondrogenic and osteogenic differentiation of rat MSCs was evaluated.MSCs were harvested from bone marrow of animals and then the response of passage1 and 2 of MSCs (P1 MSCs & P2 MSCs) to the culture in alkaline medium (pH: 8) was evaluated. Cytochemical and immunocytochemical staining were performed to distinguish chondrocytes and osteocytes. Real-time PCR was performed to evaluate the type II collagen and osteopontin mRNA levels. Results: Staining for type II collagen, a chondrocytic specific marker, revealed that after one-week culture in alkaline medium, a considerable amount of P1 MSCs had shown chondrocytic morphology. By prolonging the culture period up to 4 weeks, osteogenic cells with expanded matrix and mineralized areas around them were appeared. Results of real-time PCR showed that P1 MSCs after one week culture in alkaline medium expressed highest rate of type II collagen and osteopontin mRNA among all groups. Conclusion: This study demonstrated that alkaline medium is a potent chondrogenic differentiation inducer for MSCs in their first passage.https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/408Alkaline mediumChondrocyteMesenchymal stem cellOsteoblast |
| spellingShingle | Farshad Homayouni Moghadam Tahereh Tayebi Maryam Dehghan Gilda Eslami Hamid Nadri Alireza Moradi Hassanali Vahedian-Ardakani Kazem Barzegar Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium International Journal of Hematology-Oncology and Stem Cell Research Alkaline medium Chondrocyte Mesenchymal stem cell Osteoblast |
| title | Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium |
| title_full | Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium |
| title_fullStr | Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium |
| title_full_unstemmed | Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium |
| title_short | Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium |
| title_sort | differentiation of bone marrow mesenchymal stem cells into chondrocytes after short term culture in alkaline medium |
| topic | Alkaline medium Chondrocyte Mesenchymal stem cell Osteoblast |
| url | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/408 |
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