Bacillus coagulans SCC-19 maintains intestinal health in cadmium-exposed common carp (Cyprinus carpio L.) by strengthening the gut barriers, relieving oxidative stress and modulating the intestinal microflora
Heavy metal cadmium (Cd) pollution is a serious problem affecting the sustainable development of aquaculture and the safety of aquatic foods. Research about the use of probiotics to attenuate toxic damage caused by Cd2+ in aquatic animals has received widespread attention. Bacillus coagulans (B. coa...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321010897 |
| _version_ | 1818404441042190336 |
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| author | Xulu Chang Meiru Kang Yihao Shen Lili Yun Guokun Yang Lei Zhu Xiaolin Meng Jianxin Zhang Xi Su |
| author_facet | Xulu Chang Meiru Kang Yihao Shen Lili Yun Guokun Yang Lei Zhu Xiaolin Meng Jianxin Zhang Xi Su |
| author_sort | Xulu Chang |
| collection | DOAJ |
| description | Heavy metal cadmium (Cd) pollution is a serious problem affecting the sustainable development of aquaculture and the safety of aquatic foods. Research about the use of probiotics to attenuate toxic damage caused by Cd2+ in aquatic animals has received widespread attention. Bacillus coagulans (B. coagulans), a kind of probiotics commonly used in aquaculture, has been shown to adsorb Cd2+ both in vivo and vitro. Here, we aimed to determine the effects of B. coagulans on Cd2+ bioaccumulation, gut barrier function, oxidative stress and gut microbiota in common carp following Cd2+ exposure. The fish were exposure to Cd2+ at 0 and 0.5 mg/L and/or fed a B. coagulans-containing diet at 107, 108 and 109 CFU/g for 8 weeks. The results indicated that B. coagulans can maintain gut barrier function in Cd2+-exposed fish by reducing Cd2+ bioaccumulation, increasing the mRNA levels of tight junction protein genes (occludin, claudin-2 and zonula occludens-1), and decreasing the levels of diamine oxidase and D-lactic acid. In addition, B. coagulans could relieve oxidative stress in Cd2+-exposed fish by restoring the activities of glutathione peroxidase, catalase and superoxide dismutase. Moreover, Cd2+ exposure decreased the intestinal microbiota diversity and changed the intestinal microbiota compositions in common carp. However, supplementation with B. coagulans could reverse the altered intestinal microbiota diversity and composition after Cd2+ exposure, decrease the abundance of some pathogens (Shewanella and Vibrio), and increase the abundance of probiotics (Bacillus and Lactobacillus). These results indicate that B. coagulans may serve as a potential antidote for alleviating Cd2+ toxicity. |
| first_indexed | 2024-12-14T08:40:12Z |
| format | Article |
| id | doaj.art-9beea84da8874ac1a6c07965ae06becb |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-14T08:40:12Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-9beea84da8874ac1a6c07965ae06becb2022-12-21T23:09:19ZengElsevierEcotoxicology and Environmental Safety0147-65132021-12-01228112977Bacillus coagulans SCC-19 maintains intestinal health in cadmium-exposed common carp (Cyprinus carpio L.) by strengthening the gut barriers, relieving oxidative stress and modulating the intestinal microfloraXulu Chang0Meiru Kang1Yihao Shen2Lili Yun3Guokun Yang4Lei Zhu5Xiaolin Meng6Jianxin Zhang7Xi Su8College of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaCollege of Fisheries, Henan Normal University, Xinxiang 453007, PR ChinaHenan Mental Hospital, the Second Affiliated Hospital of Xinxiang Medical University, Xinxiang 453007, PR China; Corresponding author.Heavy metal cadmium (Cd) pollution is a serious problem affecting the sustainable development of aquaculture and the safety of aquatic foods. Research about the use of probiotics to attenuate toxic damage caused by Cd2+ in aquatic animals has received widespread attention. Bacillus coagulans (B. coagulans), a kind of probiotics commonly used in aquaculture, has been shown to adsorb Cd2+ both in vivo and vitro. Here, we aimed to determine the effects of B. coagulans on Cd2+ bioaccumulation, gut barrier function, oxidative stress and gut microbiota in common carp following Cd2+ exposure. The fish were exposure to Cd2+ at 0 and 0.5 mg/L and/or fed a B. coagulans-containing diet at 107, 108 and 109 CFU/g for 8 weeks. The results indicated that B. coagulans can maintain gut barrier function in Cd2+-exposed fish by reducing Cd2+ bioaccumulation, increasing the mRNA levels of tight junction protein genes (occludin, claudin-2 and zonula occludens-1), and decreasing the levels of diamine oxidase and D-lactic acid. In addition, B. coagulans could relieve oxidative stress in Cd2+-exposed fish by restoring the activities of glutathione peroxidase, catalase and superoxide dismutase. Moreover, Cd2+ exposure decreased the intestinal microbiota diversity and changed the intestinal microbiota compositions in common carp. However, supplementation with B. coagulans could reverse the altered intestinal microbiota diversity and composition after Cd2+ exposure, decrease the abundance of some pathogens (Shewanella and Vibrio), and increase the abundance of probiotics (Bacillus and Lactobacillus). These results indicate that B. coagulans may serve as a potential antidote for alleviating Cd2+ toxicity.http://www.sciencedirect.com/science/article/pii/S0147651321010897ProbioticsCadmium toxicityGut barrierOxidative stressIntestinal microbiota |
| spellingShingle | Xulu Chang Meiru Kang Yihao Shen Lili Yun Guokun Yang Lei Zhu Xiaolin Meng Jianxin Zhang Xi Su Bacillus coagulans SCC-19 maintains intestinal health in cadmium-exposed common carp (Cyprinus carpio L.) by strengthening the gut barriers, relieving oxidative stress and modulating the intestinal microflora Ecotoxicology and Environmental Safety Probiotics Cadmium toxicity Gut barrier Oxidative stress Intestinal microbiota |
| title | Bacillus coagulans SCC-19 maintains intestinal health in cadmium-exposed common carp (Cyprinus carpio L.) by strengthening the gut barriers, relieving oxidative stress and modulating the intestinal microflora |
| title_full | Bacillus coagulans SCC-19 maintains intestinal health in cadmium-exposed common carp (Cyprinus carpio L.) by strengthening the gut barriers, relieving oxidative stress and modulating the intestinal microflora |
| title_fullStr | Bacillus coagulans SCC-19 maintains intestinal health in cadmium-exposed common carp (Cyprinus carpio L.) by strengthening the gut barriers, relieving oxidative stress and modulating the intestinal microflora |
| title_full_unstemmed | Bacillus coagulans SCC-19 maintains intestinal health in cadmium-exposed common carp (Cyprinus carpio L.) by strengthening the gut barriers, relieving oxidative stress and modulating the intestinal microflora |
| title_short | Bacillus coagulans SCC-19 maintains intestinal health in cadmium-exposed common carp (Cyprinus carpio L.) by strengthening the gut barriers, relieving oxidative stress and modulating the intestinal microflora |
| title_sort | bacillus coagulans scc 19 maintains intestinal health in cadmium exposed common carp cyprinus carpio l by strengthening the gut barriers relieving oxidative stress and modulating the intestinal microflora |
| topic | Probiotics Cadmium toxicity Gut barrier Oxidative stress Intestinal microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321010897 |
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