An Investigation of Biochemical and Structural Changes in Reproductive System Hyperandrogenism in Experimental Letrozole-induced Polycystic Ovary Syndrome in Rat
Background and Objectives: Polycystic ovary syndrome (PCOS) is one of the most important endocrine disorders, which affects about 5-10% of women in reproductive age and is one of the main causes of infertility due to anovulation. In this study, the effect of letrozole was investigated on serum level...
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| Format: | Article |
| Language: | fas |
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Qom University of Medical Sciences
2016-11-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
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| Online Access: | http://journal.muq.ac.ir/browse.php?a_code=A-10-1-558&slc_lang=en&sid=1 |
| _version_ | 1818434943890489344 |
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| author | Azam Soleimani Mohammad Saleh Rasoli Reza Heidari Abbas Ahmadi |
| author_facet | Azam Soleimani Mohammad Saleh Rasoli Reza Heidari Abbas Ahmadi |
| author_sort | Azam Soleimani |
| collection | DOAJ |
| description | Background and Objectives: Polycystic ovary syndrome (PCOS) is one of the most important endocrine disorders, which affects about 5-10% of women in reproductive age and is one of the main causes of infertility due to anovulation. In this study, the effect of letrozole was investigated on serum levels of testosterone and estradiol hormones and body and ovarian weight.
Methods: This experimental study was carried out on 20 adult female Wistar rats (180-200 g) in 2 groups. The first group (control) received 0.2 ml of distilled water (solvent) and the second group (hyperandrogenism) received letrozole through gavage at a dose of 1 mg/kg bw for 21 days. At the end of this period, 6 mice of each group were randomly selected and sacrificed for hormonal studies. In the first step, blood samples were taken from the animals’ heart and centrifuged to prepare serum samples; also, uterine samples were prepared. The serum levels of testosterone and estradiol were measured by ELISA method. Data analysis was performed using independent t test. The significance level was considered p<0.05.
Results: According to the obtained results, the concentration of testosterone, body weight, and ovarian weight significantly increased in the group receiving Letrozole compared to the control group (p<0.05.(
Conclusion: The results of this study showed that letrozole as a non-steroidal aromatase inhibitor, blocks the conversion of testosterone into estradiol, and increases serum testosterone levels, also causes increase in body weight and ovarian weight. |
| first_indexed | 2024-12-14T16:45:02Z |
| format | Article |
| id | doaj.art-9bf21c7bb812414398ec625c679d430b |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-12-14T16:45:02Z |
| publishDate | 2016-11-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-9bf21c7bb812414398ec625c679d430b2022-12-21T22:54:12ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752016-11-01108917An Investigation of Biochemical and Structural Changes in Reproductive System Hyperandrogenism in Experimental Letrozole-induced Polycystic Ovary Syndrome in RatAzam Soleimani0Mohammad Saleh Rasoli1Reza Heidari2Abbas Ahmadi3 Urmia University Saba University Urmia University Urmia University Background and Objectives: Polycystic ovary syndrome (PCOS) is one of the most important endocrine disorders, which affects about 5-10% of women in reproductive age and is one of the main causes of infertility due to anovulation. In this study, the effect of letrozole was investigated on serum levels of testosterone and estradiol hormones and body and ovarian weight. Methods: This experimental study was carried out on 20 adult female Wistar rats (180-200 g) in 2 groups. The first group (control) received 0.2 ml of distilled water (solvent) and the second group (hyperandrogenism) received letrozole through gavage at a dose of 1 mg/kg bw for 21 days. At the end of this period, 6 mice of each group were randomly selected and sacrificed for hormonal studies. In the first step, blood samples were taken from the animals’ heart and centrifuged to prepare serum samples; also, uterine samples were prepared. The serum levels of testosterone and estradiol were measured by ELISA method. Data analysis was performed using independent t test. The significance level was considered p<0.05. Results: According to the obtained results, the concentration of testosterone, body weight, and ovarian weight significantly increased in the group receiving Letrozole compared to the control group (p<0.05.( Conclusion: The results of this study showed that letrozole as a non-steroidal aromatase inhibitor, blocks the conversion of testosterone into estradiol, and increases serum testosterone levels, also causes increase in body weight and ovarian weight.http://journal.muq.ac.ir/browse.php?a_code=A-10-1-558&slc_lang=en&sid=1polycystic ovary syndrome hyperandrogenism weights ovary letrazol |
| spellingShingle | Azam Soleimani Mohammad Saleh Rasoli Reza Heidari Abbas Ahmadi An Investigation of Biochemical and Structural Changes in Reproductive System Hyperandrogenism in Experimental Letrozole-induced Polycystic Ovary Syndrome in Rat Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum polycystic ovary syndrome hyperandrogenism weights ovary letrazol |
| title | An Investigation of Biochemical and Structural Changes in Reproductive System Hyperandrogenism in Experimental Letrozole-induced Polycystic Ovary Syndrome in Rat |
| title_full | An Investigation of Biochemical and Structural Changes in Reproductive System Hyperandrogenism in Experimental Letrozole-induced Polycystic Ovary Syndrome in Rat |
| title_fullStr | An Investigation of Biochemical and Structural Changes in Reproductive System Hyperandrogenism in Experimental Letrozole-induced Polycystic Ovary Syndrome in Rat |
| title_full_unstemmed | An Investigation of Biochemical and Structural Changes in Reproductive System Hyperandrogenism in Experimental Letrozole-induced Polycystic Ovary Syndrome in Rat |
| title_short | An Investigation of Biochemical and Structural Changes in Reproductive System Hyperandrogenism in Experimental Letrozole-induced Polycystic Ovary Syndrome in Rat |
| title_sort | investigation of biochemical and structural changes in reproductive system hyperandrogenism in experimental letrozole induced polycystic ovary syndrome in rat |
| topic | polycystic ovary syndrome hyperandrogenism weights ovary letrazol |
| url | http://journal.muq.ac.ir/browse.php?a_code=A-10-1-558&slc_lang=en&sid=1 |
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