Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control study
Abstract Background Monocyte-macrophage lineage cells are committed towards osteoclast differentiation in vitro by the downregulation of microphthalmia-induced transcription factor (MITF) by miRNA-155. Therefore, we aimed to evaluate miRNA-155 expression and explore the regulation of MITF by miRNA-1...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-022-00441-1 |
| _version_ | 1827279800023121920 |
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| author | Sowmya Reddy Nandipati Devapriya Appukuttan Sangeetha Subramanian P. S. G. Prakash |
| author_facet | Sowmya Reddy Nandipati Devapriya Appukuttan Sangeetha Subramanian P. S. G. Prakash |
| author_sort | Sowmya Reddy Nandipati |
| collection | DOAJ |
| description | Abstract Background Monocyte-macrophage lineage cells are committed towards osteoclast differentiation in vitro by the downregulation of microphthalmia-induced transcription factor (MITF) by miRNA-155. Therefore, we aimed to evaluate miRNA-155 expression and explore the regulation of MITF by miRNA-155 during osteoclastogenesis in periodontitis. Materials and methods Ninety-eight subjects were recruited and categorized into the following: group I (cases)—systemically healthy with localized stage III/IV periodontitis (N = 49) and group II (controls)—systemically and periodontally healthy (N = 49). Gingival tissue samples were procured and qRT-PCR analysis was carried out for relative gene expression. Results The mean ΔCT of miRNA-155 expression was −1.04 ± 2.26 and −0.01 ± 1.4 respectively for groups I and II. There was a statistically significant difference in the miRNA-155 expression (P ≤ 0.01) between the groups. The mean ΔCT of MITF expression for groups I and II was 4.15± 2.16 and 3.51± 1.57 respectively with no significant difference (P > 0.01) between the groups. In the periodontitis group, miRNA-155 expression increased by fivefolds (P ≤ 0.01) whereas MITF expression showed no significant difference in the fold change between the groups (P > 0.01). The site-specific clinical parameters showed a statistically significant strong negative and positive correlation with the ΔCT and fold change values of miRNA-155 respectively in the total 98 samples (P < 0.01). miRNA-155 was able to discriminate between periodontal health and disease with a diagnostic accuracy of 96.9% (95%CI: 91.38–98.95) and the AUC was 0.98 (95%CI: 0.97–1.0, SE = 0.008, P < 0.001) in ROC analysis with a sensitivity of 93.8% (95%CI: 83.48–97.9) and specificity of 100% (95%CI: 92.73–100). Conclusions miRNA-155 was dysregulated and upregulated by fivefolds in periodontal disease. It can be used as a potential biomarker to discriminate between periodontal health and disease. No difference in the MITF gene expression was demonstrated between periodontal health and disease. The result suggested that miRNA-155 does not affect the expression of MITF gene in the process of osteoclastogenesis in localized stage III/IV periodontitis within this study design and limitations. |
| first_indexed | 2024-04-11T07:18:58Z |
| format | Article |
| id | doaj.art-9bf2c9ba9a6b4776a43158ea0ad3fb5c |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:26:04Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
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| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-9bf2c9ba9a6b4776a43158ea0ad3fb5c2024-04-16T22:37:22ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202022-12-0120111010.1186/s43141-022-00441-1Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control studySowmya Reddy Nandipati0Devapriya Appukuttan1Sangeetha Subramanian2P. S. G. Prakash3Department of Periodontics, SRM Dental College and HospitalDepartment of Periodontics, SRM Dental College and HospitalDepartment of Periodontics, SRM Dental College and HospitalDepartment of Periodontics, SRM Dental College and HospitalAbstract Background Monocyte-macrophage lineage cells are committed towards osteoclast differentiation in vitro by the downregulation of microphthalmia-induced transcription factor (MITF) by miRNA-155. Therefore, we aimed to evaluate miRNA-155 expression and explore the regulation of MITF by miRNA-155 during osteoclastogenesis in periodontitis. Materials and methods Ninety-eight subjects were recruited and categorized into the following: group I (cases)—systemically healthy with localized stage III/IV periodontitis (N = 49) and group II (controls)—systemically and periodontally healthy (N = 49). Gingival tissue samples were procured and qRT-PCR analysis was carried out for relative gene expression. Results The mean ΔCT of miRNA-155 expression was −1.04 ± 2.26 and −0.01 ± 1.4 respectively for groups I and II. There was a statistically significant difference in the miRNA-155 expression (P ≤ 0.01) between the groups. The mean ΔCT of MITF expression for groups I and II was 4.15± 2.16 and 3.51± 1.57 respectively with no significant difference (P > 0.01) between the groups. In the periodontitis group, miRNA-155 expression increased by fivefolds (P ≤ 0.01) whereas MITF expression showed no significant difference in the fold change between the groups (P > 0.01). The site-specific clinical parameters showed a statistically significant strong negative and positive correlation with the ΔCT and fold change values of miRNA-155 respectively in the total 98 samples (P < 0.01). miRNA-155 was able to discriminate between periodontal health and disease with a diagnostic accuracy of 96.9% (95%CI: 91.38–98.95) and the AUC was 0.98 (95%CI: 0.97–1.0, SE = 0.008, P < 0.001) in ROC analysis with a sensitivity of 93.8% (95%CI: 83.48–97.9) and specificity of 100% (95%CI: 92.73–100). Conclusions miRNA-155 was dysregulated and upregulated by fivefolds in periodontal disease. It can be used as a potential biomarker to discriminate between periodontal health and disease. No difference in the MITF gene expression was demonstrated between periodontal health and disease. The result suggested that miRNA-155 does not affect the expression of MITF gene in the process of osteoclastogenesis in localized stage III/IV periodontitis within this study design and limitations.https://doi.org/10.1186/s43141-022-00441-1microRNAMITFmiRNA-155OsteoclastogenesisPeriodontitisqRT-PCR |
| spellingShingle | Sowmya Reddy Nandipati Devapriya Appukuttan Sangeetha Subramanian P. S. G. Prakash Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control study Journal of Genetic Engineering and Biotechnology microRNA MITF miRNA-155 Osteoclastogenesis Periodontitis qRT-PCR |
| title | Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control study |
| title_full | Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control study |
| title_fullStr | Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control study |
| title_full_unstemmed | Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control study |
| title_short | Role of miRNA-155 in the regulation of osteoclast differentiation mediated by MITF in stage III/IV periodontitis: a case-control study |
| title_sort | role of mirna 155 in the regulation of osteoclast differentiation mediated by mitf in stage iii iv periodontitis a case control study |
| topic | microRNA MITF miRNA-155 Osteoclastogenesis Periodontitis qRT-PCR |
| url | https://doi.org/10.1186/s43141-022-00441-1 |
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