Strain-Specific Effects of <i>Bifidobacterium longum</i> on Hypercholesterolemic Rats and Potential Mechanisms
Hypercholesterolemia is an independent risk factor of cardiovascular disease, which is among the major causes of death worldwide. The aim of this study was to explore whether Bifidobacterium longum strains exerted intra-species differences in cholesterol-lowering effects in hypercholesterolemic rats...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| author | Jinchi Jiang Caie Wu Chengcheng Zhang Qingsong Zhang Leilei Yu Jianxin Zhao Hao Zhang Arjan Narbad Wei Chen Qixiao Zhai |
| author_facet | Jinchi Jiang Caie Wu Chengcheng Zhang Qingsong Zhang Leilei Yu Jianxin Zhao Hao Zhang Arjan Narbad Wei Chen Qixiao Zhai |
| author_sort | Jinchi Jiang |
| collection | DOAJ |
| description | Hypercholesterolemia is an independent risk factor of cardiovascular disease, which is among the major causes of death worldwide. The aim of this study was to explore whether Bifidobacterium longum strains exerted intra-species differences in cholesterol-lowering effects in hypercholesterolemic rats and to investigate the potential mechanisms. SD rats underwent gavage with each <i>B. longum</i> strain (CCFM 1077, I3, J3 and B3) daily for 28 days. <i>B. longum</i> CCFM 1077 exerted the most potent cholesterol-lowering effect, followed by <i>B. longum</i> I3 and B3, whereas <i>B. longum</i> B3 had no effect in alleviating hypercholesterolemia. Divergent alleviation of different <i>B. longum</i> strains on hypercholesterolemia can be attributed to the differences in bile salt deconjugation ability and cholesterol assimilation ability in vitro. By 16S rRNA metagenomics analysis, the relative abundance of beneficial genus increased in the <i>B. longum</i> CCFM 1077 treatment group. The expression of key genes involved in cholesterol metabolism were also altered after the <i>B. longum</i> CCFM 1077 treatment. In conclusion, <i>B. longum</i> exhibits strain-specific effects in the alleviation of hypercholesterolemia, mainly due to differences in bacterial characteristics, bile salt deconjugation ability, cholesterol assimilation ability, expressions of key genes involved in cholesterol metabolism and alterations of gut microbiota. |
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| language | English |
| last_indexed | 2024-03-09T03:23:41Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9bf51ca2181b4cd1ab1eb97877ee584b2023-12-03T15:05:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01223130510.3390/ijms22031305Strain-Specific Effects of <i>Bifidobacterium longum</i> on Hypercholesterolemic Rats and Potential MechanismsJinchi Jiang0Caie Wu1Chengcheng Zhang2Qingsong Zhang3Leilei Yu4Jianxin Zhao5Hao Zhang6Arjan Narbad7Wei Chen8Qixiao Zhai9State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaInternational Joint Research Laboratory for Pharmabiotics & Antibiotic Resistance, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaHypercholesterolemia is an independent risk factor of cardiovascular disease, which is among the major causes of death worldwide. The aim of this study was to explore whether Bifidobacterium longum strains exerted intra-species differences in cholesterol-lowering effects in hypercholesterolemic rats and to investigate the potential mechanisms. SD rats underwent gavage with each <i>B. longum</i> strain (CCFM 1077, I3, J3 and B3) daily for 28 days. <i>B. longum</i> CCFM 1077 exerted the most potent cholesterol-lowering effect, followed by <i>B. longum</i> I3 and B3, whereas <i>B. longum</i> B3 had no effect in alleviating hypercholesterolemia. Divergent alleviation of different <i>B. longum</i> strains on hypercholesterolemia can be attributed to the differences in bile salt deconjugation ability and cholesterol assimilation ability in vitro. By 16S rRNA metagenomics analysis, the relative abundance of beneficial genus increased in the <i>B. longum</i> CCFM 1077 treatment group. The expression of key genes involved in cholesterol metabolism were also altered after the <i>B. longum</i> CCFM 1077 treatment. In conclusion, <i>B. longum</i> exhibits strain-specific effects in the alleviation of hypercholesterolemia, mainly due to differences in bacterial characteristics, bile salt deconjugation ability, cholesterol assimilation ability, expressions of key genes involved in cholesterol metabolism and alterations of gut microbiota.https://www.mdpi.com/1422-0067/22/3/1305<i>B. longum</i> strainshypercholesterolemiastrain-specificbile salt deconjugationcholesterol assimilationgut microbiota |
| spellingShingle | Jinchi Jiang Caie Wu Chengcheng Zhang Qingsong Zhang Leilei Yu Jianxin Zhao Hao Zhang Arjan Narbad Wei Chen Qixiao Zhai Strain-Specific Effects of <i>Bifidobacterium longum</i> on Hypercholesterolemic Rats and Potential Mechanisms International Journal of Molecular Sciences <i>B. longum</i> strains hypercholesterolemia strain-specific bile salt deconjugation cholesterol assimilation gut microbiota |
| title | Strain-Specific Effects of <i>Bifidobacterium longum</i> on Hypercholesterolemic Rats and Potential Mechanisms |
| title_full | Strain-Specific Effects of <i>Bifidobacterium longum</i> on Hypercholesterolemic Rats and Potential Mechanisms |
| title_fullStr | Strain-Specific Effects of <i>Bifidobacterium longum</i> on Hypercholesterolemic Rats and Potential Mechanisms |
| title_full_unstemmed | Strain-Specific Effects of <i>Bifidobacterium longum</i> on Hypercholesterolemic Rats and Potential Mechanisms |
| title_short | Strain-Specific Effects of <i>Bifidobacterium longum</i> on Hypercholesterolemic Rats and Potential Mechanisms |
| title_sort | strain specific effects of i bifidobacterium longum i on hypercholesterolemic rats and potential mechanisms |
| topic | <i>B. longum</i> strains hypercholesterolemia strain-specific bile salt deconjugation cholesterol assimilation gut microbiota |
| url | https://www.mdpi.com/1422-0067/22/3/1305 |
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