Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients
Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethyl-ligand extracts in allergic patients and health...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2023-10-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100670&tlng=en |
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| author | A.S. Correa J.S. Miranda L.A.R. Oliveira P.F.S. Moreira F.A.M. Vieira J.P. Cunha-Junior R.O. Resende E.A. Taketomi |
| author_facet | A.S. Correa J.S. Miranda L.A.R. Oliveira P.F.S. Moreira F.A.M. Vieira J.P. Cunha-Junior R.O. Resende E.A. Taketomi |
| author_sort | A.S. Correa |
| collection | DOAJ |
| description | Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethyl-ligand extracts in allergic patients and healthy individuals. Ion exchange carboxymethyl (CM) chromatography (CM-Sepharose) was used to isolate proteins (S2) from L. multiflorum crude extract (S1), which were assessed by SDS-PAGE. S1- and S2-specific IgE and IgG4 levels were measured by ELISA using sera from 55 atopic and 16 non-atopic subjects. Reactive polypeptide bands in S1 and S2 were detected by immunoblotting, and the most prominent bands in S2 were analyzed by mass spectrometry (MS-MS). Similar IgE and IgG4 levels were observed to both S1 (IgE median absorbance: 1.22; IgG4 median absorbance: 0.68) and S2 (IgE median absorbance: 1.26; IgG4 median absorbance: 0.85) in atopic subjects. S1 and S2 had positive correlations for IgE and IgG4 (IgE: r=0.9567; IgG4: r=0.9229; P<0.0001) levels. Homology between S1 and S2 was confirmed by IgE (84%) and IgG4 (83%) inhibition. Immunoblotting revealed that the 29-32 kDa band was recognized by 100% of atopic subjects in both S1 and S2. MS-MS analysis identified similarity profile to groups 1 and 5 grass allergens. This study revealed that carboxymethyl-ligand fraction played an important role for pollen allergy diagnosis by containing clinically relevant allergens and constituted a promising candidate for allergen-specific immunotherapy. |
| first_indexed | 2024-03-11T18:05:20Z |
| format | Article |
| id | doaj.art-9bf666e92d34469b88a47ce95281586a |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-03-11T18:05:20Z |
| publishDate | 2023-10-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-9bf666e92d34469b88a47ce95281586a2023-10-17T07:39:38ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2023-10-015610.1590/1414-431x2023e12957Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patientsA.S. Correahttps://orcid.org/0000-0002-1370-0714J.S. Mirandahttps://orcid.org/0000-0002-2182-0863L.A.R. Oliveirahttps://orcid.org/0000-0001-8509-8964P.F.S. Moreirahttps://orcid.org/0000-0003-1446-5063F.A.M. Vieirahttps://orcid.org/0009-0008-5749-5629J.P. Cunha-Juniorhttps://orcid.org/0000-0001-6182-4298R.O. Resendehttps://orcid.org/0000-0002-6711-6353E.A. Taketomihttps://orcid.org/0000-0003-4854-6822Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethyl-ligand extracts in allergic patients and healthy individuals. Ion exchange carboxymethyl (CM) chromatography (CM-Sepharose) was used to isolate proteins (S2) from L. multiflorum crude extract (S1), which were assessed by SDS-PAGE. S1- and S2-specific IgE and IgG4 levels were measured by ELISA using sera from 55 atopic and 16 non-atopic subjects. Reactive polypeptide bands in S1 and S2 were detected by immunoblotting, and the most prominent bands in S2 were analyzed by mass spectrometry (MS-MS). Similar IgE and IgG4 levels were observed to both S1 (IgE median absorbance: 1.22; IgG4 median absorbance: 0.68) and S2 (IgE median absorbance: 1.26; IgG4 median absorbance: 0.85) in atopic subjects. S1 and S2 had positive correlations for IgE and IgG4 (IgE: r=0.9567; IgG4: r=0.9229; P<0.0001) levels. Homology between S1 and S2 was confirmed by IgE (84%) and IgG4 (83%) inhibition. Immunoblotting revealed that the 29-32 kDa band was recognized by 100% of atopic subjects in both S1 and S2. MS-MS analysis identified similarity profile to groups 1 and 5 grass allergens. This study revealed that carboxymethyl-ligand fraction played an important role for pollen allergy diagnosis by containing clinically relevant allergens and constituted a promising candidate for allergen-specific immunotherapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100670&tlng=enAllergenIon-exchange chromatographyBeta-expansinLolium multiflorumIgEIgG4 |
| spellingShingle | A.S. Correa J.S. Miranda L.A.R. Oliveira P.F.S. Moreira F.A.M. Vieira J.P. Cunha-Junior R.O. Resende E.A. Taketomi Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients Brazilian Journal of Medical and Biological Research Allergen Ion-exchange chromatography Beta-expansin Lolium multiflorum IgE IgG4 |
| title | Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients |
| title_full | Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients |
| title_fullStr | Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients |
| title_full_unstemmed | Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients |
| title_short | Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients |
| title_sort | identification of carboxymethyl cm binding proteins derived from lolium multiflorum pollen extract and antibody reactivity in brazilian allergic patients |
| topic | Allergen Ion-exchange chromatography Beta-expansin Lolium multiflorum IgE IgG4 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100670&tlng=en |
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