In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue
The objective: to investigate the anti-tuberculosis effect of laser photodynamic inactivation (PDI) of M. tuberculosis H37Rv in vitro by methylene blue (MB) in the minimum concentration (1 μg/ml) with laser radiation of 662 nm. Subjects and methods. A comparative analysis of the intensity of growth...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
New Terra Publishing House
2019-08-01
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| Series: | Туберкулез и болезни лёгких |
| Subjects: | |
| Online Access: | https://www.tibl-journal.com/jour/article/view/1312 |
| Summary: | The objective: to investigate the anti-tuberculosis effect of laser photodynamic inactivation (PDI) of M. tuberculosis H37Rv in vitro by methylene blue (MB) in the minimum concentration (1 μg/ml) with laser radiation of 662 nm. Subjects and methods. A comparative analysis of the intensity of growth of Mycobacterium tuberculosis H37Rv after laser irradiation and laser FDI by MB with different doses of light energy was carried out. Results. Laser radiation with a wavelength of 662 nm was found to have an inhibitory effect on the growth of M. tuberculosis H37Rv. FDI of Mycobacterium tuberculosis was first registered in the presence of a minimum concentration of MB (1 µg/ml) which suppressed colony growth by 97 and 93% when they were processed by radiation with a wavelength of 662 nm with the lowest density of doses of light energy (46.9 and 93.75 J/cm2). |
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| ISSN: | 2075-1230 2542-1506 |