In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue
The objective: to investigate the anti-tuberculosis effect of laser photodynamic inactivation (PDI) of M. tuberculosis H37Rv in vitro by methylene blue (MB) in the minimum concentration (1 μg/ml) with laser radiation of 662 nm. Subjects and methods. A comparative analysis of the intensity of growth...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2019-08-01
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| Series: | Туберкулез и болезни лёгких |
| Subjects: | |
| Online Access: | https://www.tibl-journal.com/jour/article/view/1312 |
| _version_ | 1826563060428439552 |
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| author | D. A. Bredikhin S. D. Nikonov A. G. Cherednichenko T. I. Petrenko A. I. Korbut |
| author_facet | D. A. Bredikhin S. D. Nikonov A. G. Cherednichenko T. I. Petrenko A. I. Korbut |
| author_sort | D. A. Bredikhin |
| collection | DOAJ |
| description | The objective: to investigate the anti-tuberculosis effect of laser photodynamic inactivation (PDI) of M. tuberculosis H37Rv in vitro by methylene blue (MB) in the minimum concentration (1 μg/ml) with laser radiation of 662 nm. Subjects and methods. A comparative analysis of the intensity of growth of Mycobacterium tuberculosis H37Rv after laser irradiation and laser FDI by MB with different doses of light energy was carried out. Results. Laser radiation with a wavelength of 662 nm was found to have an inhibitory effect on the growth of M. tuberculosis H37Rv. FDI of Mycobacterium tuberculosis was first registered in the presence of a minimum concentration of MB (1 µg/ml) which suppressed colony growth by 97 and 93% when they were processed by radiation with a wavelength of 662 nm with the lowest density of doses of light energy (46.9 and 93.75 J/cm2). |
| first_indexed | 2024-03-12T03:30:05Z |
| format | Article |
| id | doaj.art-9bf7c3897dfe4e22aeba4117faad55da |
| institution | Directory Open Access Journal |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| last_indexed | 2025-03-14T09:57:58Z |
| publishDate | 2019-08-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj.art-9bf7c3897dfe4e22aeba4117faad55da2025-03-02T11:22:31ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062019-08-01977283310.21292/2075-1230-2019-97-7-28-331312In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blueD. A. Bredikhin0S. D. Nikonov1A. G. Cherednichenko2T. I. Petrenko3A. I. Korbut4Novosibirsk State University; Novosibirsk State Regional Clinical Tuberculosis HospitalNovosibirsk Tuberculosis Research Institute; Novosibirsk State University; Novosibirsk State Regional Clinical Tuberculosis HospitalNovosibirsk Tuberculosis Research Institute; Novosibirsk State UniversityNovosibirsk Tuberculosis Research InstituteResearch Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center of Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of SciencesThe objective: to investigate the anti-tuberculosis effect of laser photodynamic inactivation (PDI) of M. tuberculosis H37Rv in vitro by methylene blue (MB) in the minimum concentration (1 μg/ml) with laser radiation of 662 nm. Subjects and methods. A comparative analysis of the intensity of growth of Mycobacterium tuberculosis H37Rv after laser irradiation and laser FDI by MB with different doses of light energy was carried out. Results. Laser radiation with a wavelength of 662 nm was found to have an inhibitory effect on the growth of M. tuberculosis H37Rv. FDI of Mycobacterium tuberculosis was first registered in the presence of a minimum concentration of MB (1 µg/ml) which suppressed colony growth by 97 and 93% when they were processed by radiation with a wavelength of 662 nm with the lowest density of doses of light energy (46.9 and 93.75 J/cm2).https://www.tibl-journal.com/jour/article/view/1312anti-microbial photodynamic therapytuberculosismycobacterium tuberculosis h37rvmethylene blue |
| spellingShingle | D. A. Bredikhin S. D. Nikonov A. G. Cherednichenko T. I. Petrenko A. I. Korbut In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue Туберкулез и болезни лёгких anti-microbial photodynamic therapy tuberculosis mycobacterium tuberculosis h37rv methylene blue |
| title | In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue |
| title_full | In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue |
| title_fullStr | In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue |
| title_full_unstemmed | In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue |
| title_short | In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue |
| title_sort | in vitro photodynamic inactivation of mycobacterium tuberculosis by methylene blue |
| topic | anti-microbial photodynamic therapy tuberculosis mycobacterium tuberculosis h37rv methylene blue |
| url | https://www.tibl-journal.com/jour/article/view/1312 |
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