10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees
Abstract In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fu...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-89212-5 |
| _version_ | 1819202714883588096 |
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| author | Luiza Diniz Ferreira Borges Letícia Leandro Batista Serena Mares Malta Tamiris Sabrina Rodrigues Jéssica Regina da Costa Silva Gabriela Venturini Alexandre da Costa Pereira Pedro Henrique Gonçalves Guedes Carlos Ueira-Vieira Ana Maria Bonetti |
| author_facet | Luiza Diniz Ferreira Borges Letícia Leandro Batista Serena Mares Malta Tamiris Sabrina Rodrigues Jéssica Regina da Costa Silva Gabriela Venturini Alexandre da Costa Pereira Pedro Henrique Gonçalves Guedes Carlos Ueira-Vieira Ana Maria Bonetti |
| author_sort | Luiza Diniz Ferreira Borges |
| collection | DOAJ |
| description | Abstract In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we verified that the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A were expressed at all stages of Melipona scutellaris, with fluctuations between developmental stages and castes. In larvae, the HDAC genes showed the same profile of Juvenile Hormone titers—previous reported—whereas the HAT gene exhibited the opposite profile. We also investigated the larvae and larval food metabolomes, but we did not identify the putative queen-fate inducing compounds, geraniol and 10-hydroxy-2E-decenoic acid (10HDA). Finally, we demonstrated that the histone deacetylase inhibitor 10HDA—the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation—was unable to promote differentiation in queens in Melipona scutellaris. Our results suggest that epigenetic and hormonal regulations may act synergistically to drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris. |
| first_indexed | 2024-12-23T04:08:25Z |
| format | Article |
| id | doaj.art-9bfdd03ce58844a2829a686d6bd39021 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T04:08:25Z |
| publishDate | 2021-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9bfdd03ce58844a2829a686d6bd390212022-12-21T18:00:34ZengNature PortfolioScientific Reports2045-23222021-05-0111111010.1038/s41598-021-89212-510-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless beesLuiza Diniz Ferreira Borges0Letícia Leandro Batista1Serena Mares Malta2Tamiris Sabrina Rodrigues3Jéssica Regina da Costa Silva4Gabriela Venturini5Alexandre da Costa Pereira6Pedro Henrique Gonçalves Guedes7Carlos Ueira-Vieira8Ana Maria Bonetti9Institute of Biotechnology, Federal University of UberlândiaInstitute of Biotechnology, Federal University of UberlândiaInstitute of Biotechnology, Federal University of UberlândiaInstitute of Biotechnology, Federal University of UberlândiaInstitute of Biotechnology, Federal University of UberlândiaDepartment of Genetics, Harvard Medical SchoolDepartment of Genetics, Harvard Medical SchoolInstitute of Biotechnology, Federal University of UberlândiaInstitute of Biotechnology, Federal University of UberlândiaInstitute of Biotechnology, Federal University of UberlândiaAbstract In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we verified that the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A were expressed at all stages of Melipona scutellaris, with fluctuations between developmental stages and castes. In larvae, the HDAC genes showed the same profile of Juvenile Hormone titers—previous reported—whereas the HAT gene exhibited the opposite profile. We also investigated the larvae and larval food metabolomes, but we did not identify the putative queen-fate inducing compounds, geraniol and 10-hydroxy-2E-decenoic acid (10HDA). Finally, we demonstrated that the histone deacetylase inhibitor 10HDA—the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation—was unable to promote differentiation in queens in Melipona scutellaris. Our results suggest that epigenetic and hormonal regulations may act synergistically to drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.https://doi.org/10.1038/s41598-021-89212-5 |
| spellingShingle | Luiza Diniz Ferreira Borges Letícia Leandro Batista Serena Mares Malta Tamiris Sabrina Rodrigues Jéssica Regina da Costa Silva Gabriela Venturini Alexandre da Costa Pereira Pedro Henrique Gonçalves Guedes Carlos Ueira-Vieira Ana Maria Bonetti 10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees Scientific Reports |
| title | 10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees |
| title_full | 10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees |
| title_fullStr | 10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees |
| title_full_unstemmed | 10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees |
| title_short | 10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees |
| title_sort | 10 hydroxy 2e decenoic acid 10hda does not promote caste differentiation in melipona scutellaris stingless bees |
| url | https://doi.org/10.1038/s41598-021-89212-5 |
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