Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing

Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing

The naturally occurring prokaryotic CRISPR-Cas systems provide valuable resources for the development of new genome-editing tools. However, the majority of prokaryotic Cas nucleases exhibit poor editing efficiency in mammalian cells, which significantly limits their utility. Here, we have developed...

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Bibliographic Details
Main Authors: Yangcan Chen, Yanping Hu, Xinge Wang, Shengqiu Luo, Ning Yang, Yi Chen, Zhikun Li, Qi Zhou, Wei Li
Format: Article
Language:English
Published: Elsevier 2022-07-01
Series:The Innovation
Subjects:
protein engineering
genome editing
CRISPR-Cas12i
simple PAM requirement
high fidelity
Online Access:http://www.sciencedirect.com/science/article/pii/S2666675822000601

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