Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors
Surface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/1/32 |
| _version_ | 1797495482802503680 |
|---|---|
| author | Evelina I. Nikelshparg Adil A. Baizhumanov Zhanna V. Bochkova Sergey M. Novikov Dmitry I. Yakubovsky Aleksey V. Arsenin Valentyn S. Volkov Eugene A. Goodilin Anna A. Semenova Olga Sosnovtseva Georgy V. Maksimov Nadezda A. Brazhe |
| author_facet | Evelina I. Nikelshparg Adil A. Baizhumanov Zhanna V. Bochkova Sergey M. Novikov Dmitry I. Yakubovsky Aleksey V. Arsenin Valentyn S. Volkov Eugene A. Goodilin Anna A. Semenova Olga Sosnovtseva Georgy V. Maksimov Nadezda A. Brazhe |
| author_sort | Evelina I. Nikelshparg |
| collection | DOAJ |
| description | Surface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can provide a better understanding of CVD mechanisms, and the monitoring of the identified molecular changes can be employed in the development of novel biosensor tools for early diagnostics. We applied plasmonic SERS nanosensors to assess changes in the properties of erythrocytes under normotensive and hypertensive conditions in the animal model. We found that spontaneous hypertension in rats leads (i) to a decrease in the erythrocyte plasma membrane fluidity and (ii) to a decrease in the mobility of the heme of the membrane-bound hemoglobin. We identified SERS parameters that can be used to detect pathological changes in the plasma membrane and submembrane region of erythrocytes. |
| first_indexed | 2024-03-10T01:49:17Z |
| format | Article |
| id | doaj.art-9bff2d91372644a6bc8679bcb9ca12e3 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T01:49:17Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-9bff2d91372644a6bc8679bcb9ca12e32023-11-23T13:08:16ZengMDPI AGBiosensors2079-63742022-01-011213210.3390/bios12010032Detection of Hypertension-Induced Changes in Erythrocytes by SERS NanosensorsEvelina I. Nikelshparg0Adil A. Baizhumanov1Zhanna V. Bochkova2Sergey M. Novikov3Dmitry I. Yakubovsky4Aleksey V. Arsenin5Valentyn S. Volkov6Eugene A. Goodilin7Anna A. Semenova8Olga Sosnovtseva9Georgy V. Maksimov10Nadezda A. Brazhe11Department of Biophysics, Biological Faculty, Moscow State University, 119991 Moscow, RussiaDepartment of Biophysics, Biological Faculty, Moscow State University, 119991 Moscow, RussiaDepartment of Biophysics, Biological Faculty, Moscow State University, 119991 Moscow, RussiaCenter for Photonics and 2D Materials, Moscow Institute of Physics and Technology (MIPT), 141700 Dolgoprudny, RussiaCenter for Photonics and 2D Materials, Moscow Institute of Physics and Technology (MIPT), 141700 Dolgoprudny, RussiaCenter for Photonics and 2D Materials, Moscow Institute of Physics and Technology (MIPT), 141700 Dolgoprudny, RussiaCenter for Photonics and 2D Materials, Moscow Institute of Physics and Technology (MIPT), 141700 Dolgoprudny, RussiaFaculty of Materials Sciences, Moscow State University, 119991 Moscow, RussiaFaculty of Materials Sciences, Moscow State University, 119991 Moscow, RussiaDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, DenmarkDepartment of Biophysics, Biological Faculty, Moscow State University, 119991 Moscow, RussiaDepartment of Biophysics, Biological Faculty, Moscow State University, 119991 Moscow, RussiaSurface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can provide a better understanding of CVD mechanisms, and the monitoring of the identified molecular changes can be employed in the development of novel biosensor tools for early diagnostics. We applied plasmonic SERS nanosensors to assess changes in the properties of erythrocytes under normotensive and hypertensive conditions in the animal model. We found that spontaneous hypertension in rats leads (i) to a decrease in the erythrocyte plasma membrane fluidity and (ii) to a decrease in the mobility of the heme of the membrane-bound hemoglobin. We identified SERS parameters that can be used to detect pathological changes in the plasma membrane and submembrane region of erythrocytes.https://www.mdpi.com/2079-6374/12/1/32surface-enhanced Raman spectroscopyhemoglobinerythrocytesplasma membranebiosensingnanoparticles |
| spellingShingle | Evelina I. Nikelshparg Adil A. Baizhumanov Zhanna V. Bochkova Sergey M. Novikov Dmitry I. Yakubovsky Aleksey V. Arsenin Valentyn S. Volkov Eugene A. Goodilin Anna A. Semenova Olga Sosnovtseva Georgy V. Maksimov Nadezda A. Brazhe Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors Biosensors surface-enhanced Raman spectroscopy hemoglobin erythrocytes plasma membrane biosensing nanoparticles |
| title | Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors |
| title_full | Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors |
| title_fullStr | Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors |
| title_full_unstemmed | Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors |
| title_short | Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors |
| title_sort | detection of hypertension induced changes in erythrocytes by sers nanosensors |
| topic | surface-enhanced Raman spectroscopy hemoglobin erythrocytes plasma membrane biosensing nanoparticles |
| url | https://www.mdpi.com/2079-6374/12/1/32 |
| work_keys_str_mv | AT evelinainikelshparg detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT adilabaizhumanov detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT zhannavbochkova detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT sergeymnovikov detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT dmitryiyakubovsky detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT alekseyvarsenin detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT valentynsvolkov detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT eugeneagoodilin detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT annaasemenova detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT olgasosnovtseva detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT georgyvmaksimov detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors AT nadezdaabrazhe detectionofhypertensioninducedchangesinerythrocytesbysersnanosensors |