The 16S rRNA Analysis and Enzyme Screening of Bacillus from Rhizosphere Soil of Lombok Island
Bacillus are commonly found in nature, especially in soil and food. It has the ability to produce bioactive compounds as well as the enzyme. This study was aimed to isolate, identify, and screen their enzyme activities. Four soil samples from Mandalika, Lombok Island, West Nusa Tenggara (NTB), were...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2021-10-01
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| Series: | Jurnal Ilmu Pertanian Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/JIPI/article/view/34166 |
| _version_ | 1828945674889068544 |
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| author | Tri Ratna Sulistiyani Mia Kusmiati Gita Azizah Putri |
| author_facet | Tri Ratna Sulistiyani Mia Kusmiati Gita Azizah Putri |
| author_sort | Tri Ratna Sulistiyani |
| collection | DOAJ |
| description | Bacillus are commonly found in nature, especially in soil and food. It has the ability to produce bioactive compounds as well as the enzyme. This study was aimed to isolate, identify, and screen their enzyme activities. Four soil samples from Mandalika, Lombok Island, West Nusa Tenggara (NTB), were used for isolation. Bacillus was isolated using the heat-shock method and characterized through Gram staining, endospore staining, and morphological phenotype. Bacillus identification was conducted based on 16S rRNA gene sequence. The hydrolytic enzyme activities were checked qualitatively using selective media, and the enzyme tested including amylase, galactosidase, lipase, protease, and cellulase. As many as twenty-two bacteria isolates were obtained from four soil samples and represented 15 distinct species. The member of bacteria genera successfully identified, consisted of Bacillus sp., Brevibacillus sp., and Fictibacillus sp. Bacillus sp. was the most isolated. Some of the isolated bacteria have the ability to produce lipase, protease, and cellulase that potential to be used in biotechnology processes.
Keywords: Bacillus, hydrolytic enzymes, identification, screening |
| first_indexed | 2024-12-14T04:57:04Z |
| format | Article |
| id | doaj.art-9bff76fc49ab48fa85478d07b43842ee |
| institution | Directory Open Access Journal |
| issn | 0853-4217 2443-3462 |
| language | English |
| last_indexed | 2024-12-14T04:57:04Z |
| publishDate | 2021-10-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Jurnal Ilmu Pertanian Indonesia |
| spelling | doaj.art-9bff76fc49ab48fa85478d07b43842ee2022-12-21T23:16:20ZengBogor Agricultural UniversityJurnal Ilmu Pertanian Indonesia0853-42172443-34622021-10-0126410.18343/jipi.26.4.582The 16S rRNA Analysis and Enzyme Screening of Bacillus from Rhizosphere Soil of Lombok IslandTri Ratna Sulistiyani0Mia Kusmiati1Gita Azizah Putri2Research Center for Biology-Indonesian Institute of Sciences (LIPI), Jl. Raya Jakarta Bogor Km. 46 Cibinong, 16911Research Center for Biology-Indonesian Institute of Sciences (LIPI), Jl. Raya Jakarta Bogor Km. 46 Cibinong, 16911Research Center for Biology-Indonesian Institute of Sciences (LIPI), Jl. Raya Jakarta Bogor Km. 46 Cibinong, 16911Bacillus are commonly found in nature, especially in soil and food. It has the ability to produce bioactive compounds as well as the enzyme. This study was aimed to isolate, identify, and screen their enzyme activities. Four soil samples from Mandalika, Lombok Island, West Nusa Tenggara (NTB), were used for isolation. Bacillus was isolated using the heat-shock method and characterized through Gram staining, endospore staining, and morphological phenotype. Bacillus identification was conducted based on 16S rRNA gene sequence. The hydrolytic enzyme activities were checked qualitatively using selective media, and the enzyme tested including amylase, galactosidase, lipase, protease, and cellulase. As many as twenty-two bacteria isolates were obtained from four soil samples and represented 15 distinct species. The member of bacteria genera successfully identified, consisted of Bacillus sp., Brevibacillus sp., and Fictibacillus sp. Bacillus sp. was the most isolated. Some of the isolated bacteria have the ability to produce lipase, protease, and cellulase that potential to be used in biotechnology processes. Keywords: Bacillus, hydrolytic enzymes, identification, screeninghttps://journal.ipb.ac.id/index.php/JIPI/article/view/34166 |
| spellingShingle | Tri Ratna Sulistiyani Mia Kusmiati Gita Azizah Putri The 16S rRNA Analysis and Enzyme Screening of Bacillus from Rhizosphere Soil of Lombok Island Jurnal Ilmu Pertanian Indonesia |
| title | The 16S rRNA Analysis and Enzyme Screening of Bacillus from Rhizosphere Soil of Lombok Island |
| title_full | The 16S rRNA Analysis and Enzyme Screening of Bacillus from Rhizosphere Soil of Lombok Island |
| title_fullStr | The 16S rRNA Analysis and Enzyme Screening of Bacillus from Rhizosphere Soil of Lombok Island |
| title_full_unstemmed | The 16S rRNA Analysis and Enzyme Screening of Bacillus from Rhizosphere Soil of Lombok Island |
| title_short | The 16S rRNA Analysis and Enzyme Screening of Bacillus from Rhizosphere Soil of Lombok Island |
| title_sort | 16s rrna analysis and enzyme screening of bacillus from rhizosphere soil of lombok island |
| url | https://journal.ipb.ac.id/index.php/JIPI/article/view/34166 |
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