Optimal dose of Gamma radiation for <i>Phaseolus vulgaris</i> L. cultivar 'BAT-93' <i>in vitro</i> plants regeneration
The application of Gamma radiations combined with tissues cultures in common bean constitutes a tool to accelerate the programs of genetic improvement. The determination of the optimal radiation dose is an important aspect to be defined in order to generate genetic variability. For this reason, the...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2018-10-01
|
| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/572 |
| _version_ | 1818245526631481344 |
|---|---|
| author | Amanda Martirena-Ramírez Novisel Veitía Lourdes R García Raúl Collado Damaris Torres Leonardo Rivero Miriam Ramírez-López |
| author_facet | Amanda Martirena-Ramírez Novisel Veitía Lourdes R García Raúl Collado Damaris Torres Leonardo Rivero Miriam Ramírez-López |
| author_sort | Amanda Martirena-Ramírez |
| collection | DOAJ |
| description | The application of Gamma radiations combined with tissues cultures in common bean constitutes a tool to accelerate the programs of genetic improvement. The determination of the optimal radiation dose is an important aspect to be defined in order to generate genetic variability. For this reason, the objective of this study was to determine the optimal dose of Gamma radiation for regenerating Phaseolus vulgaris L. cultivar 'BAT-93'in vitro plants. It were applied doses of 20, 30, 40, 50 or 60, Gy to mature seeds and an unirradiated control was included. The seeds were in vitro germinated and the cotyledonary nodes with two cotyledon was used for callus formation. Per mutagenic treatment and the control without irradiation 100 seeds were used. The evaluations were made 21 days after the radiation was applied. The percentage of explants that formed calluses and its fresh weight (g) were recorded. With these values, the mean lethal dose (LD50) was determined. In the regeneration, the number of shoots per callus was determined, from these values the mean Reductive Dosage (GR50) was calculated. The main affectations occurred in the callus formation and in the shoots regeneration. The dose of 30 Gy was selected for the massive treatments of common bean cv. 'BAT-93' seeds with Gamma radiations. This dose was closer to the LD50 and GR50). Keywords:in vitro culture, common bean, radiosensitivity, seeds |
| first_indexed | 2024-12-12T14:34:19Z |
| format | Article |
| id | doaj.art-9bffb3741fd647149fe167bed82d4abf |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-12T14:34:19Z |
| publishDate | 2018-10-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-9bffb3741fd647149fe167bed82d4abf2022-12-22T00:21:24ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472018-10-01181526Optimal dose of Gamma radiation for <i>Phaseolus vulgaris</i> L. cultivar 'BAT-93' <i>in vitro</i> plants regenerationAmanda Martirena-Ramírez0Novisel VeitíaLourdes R GarcíaRaúl ColladoDamaris TorresLeonardo RiveroMiriam Ramírez-LópezInstituto de Biotecnología de las PlantasThe application of Gamma radiations combined with tissues cultures in common bean constitutes a tool to accelerate the programs of genetic improvement. The determination of the optimal radiation dose is an important aspect to be defined in order to generate genetic variability. For this reason, the objective of this study was to determine the optimal dose of Gamma radiation for regenerating Phaseolus vulgaris L. cultivar 'BAT-93'in vitro plants. It were applied doses of 20, 30, 40, 50 or 60, Gy to mature seeds and an unirradiated control was included. The seeds were in vitro germinated and the cotyledonary nodes with two cotyledon was used for callus formation. Per mutagenic treatment and the control without irradiation 100 seeds were used. The evaluations were made 21 days after the radiation was applied. The percentage of explants that formed calluses and its fresh weight (g) were recorded. With these values, the mean lethal dose (LD50) was determined. In the regeneration, the number of shoots per callus was determined, from these values the mean Reductive Dosage (GR50) was calculated. The main affectations occurred in the callus formation and in the shoots regeneration. The dose of 30 Gy was selected for the massive treatments of common bean cv. 'BAT-93' seeds with Gamma radiations. This dose was closer to the LD50 and GR50). Keywords:in vitro culture, common bean, radiosensitivity, seedshttps://revista.ibp.co.cu/index.php/BV/article/view/572 |
| spellingShingle | Amanda Martirena-Ramírez Novisel Veitía Lourdes R García Raúl Collado Damaris Torres Leonardo Rivero Miriam Ramírez-López Optimal dose of Gamma radiation for <i>Phaseolus vulgaris</i> L. cultivar 'BAT-93' <i>in vitro</i> plants regeneration Biotecnología Vegetal |
| title | Optimal dose of Gamma radiation for <i>Phaseolus vulgaris</i> L. cultivar 'BAT-93' <i>in vitro</i> plants regeneration |
| title_full | Optimal dose of Gamma radiation for <i>Phaseolus vulgaris</i> L. cultivar 'BAT-93' <i>in vitro</i> plants regeneration |
| title_fullStr | Optimal dose of Gamma radiation for <i>Phaseolus vulgaris</i> L. cultivar 'BAT-93' <i>in vitro</i> plants regeneration |
| title_full_unstemmed | Optimal dose of Gamma radiation for <i>Phaseolus vulgaris</i> L. cultivar 'BAT-93' <i>in vitro</i> plants regeneration |
| title_short | Optimal dose of Gamma radiation for <i>Phaseolus vulgaris</i> L. cultivar 'BAT-93' <i>in vitro</i> plants regeneration |
| title_sort | optimal dose of gamma radiation for i phaseolus vulgaris i l cultivar bat 93 i in vitro i plants regeneration |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/572 |
| work_keys_str_mv | AT amandamartirenaramirez optimaldoseofgammaradiationforiphaseolusvulgarisilcultivarbat93iinvitroiplantsregeneration AT noviselveitia optimaldoseofgammaradiationforiphaseolusvulgarisilcultivarbat93iinvitroiplantsregeneration AT lourdesrgarcia optimaldoseofgammaradiationforiphaseolusvulgarisilcultivarbat93iinvitroiplantsregeneration AT raulcollado optimaldoseofgammaradiationforiphaseolusvulgarisilcultivarbat93iinvitroiplantsregeneration AT damaristorres optimaldoseofgammaradiationforiphaseolusvulgarisilcultivarbat93iinvitroiplantsregeneration AT leonardorivero optimaldoseofgammaradiationforiphaseolusvulgarisilcultivarbat93iinvitroiplantsregeneration AT miriamramirezlopez optimaldoseofgammaradiationforiphaseolusvulgarisilcultivarbat93iinvitroiplantsregeneration |