Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescence
Abstract Cellular senescence is a phenotype characterized by cessation of cell division, which can be caused by exhaustive replication or environmental stress. It is involved in age-related pathophysiological conditions and affects both the cellular cytoskeleton and the prime cellular mechanosensors...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-36347-2 |
| _version_ | 1797801452873187328 |
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| author | Carolin Grandy Fabian Port Meytal Radzinski Karmveer Singh Dorothee Erz Jonas Pfeil Dana Reichmann Kay-Eberhard Gottschalk |
| author_facet | Carolin Grandy Fabian Port Meytal Radzinski Karmveer Singh Dorothee Erz Jonas Pfeil Dana Reichmann Kay-Eberhard Gottschalk |
| author_sort | Carolin Grandy |
| collection | DOAJ |
| description | Abstract Cellular senescence is a phenotype characterized by cessation of cell division, which can be caused by exhaustive replication or environmental stress. It is involved in age-related pathophysiological conditions and affects both the cellular cytoskeleton and the prime cellular mechanosensors, focal adhesion complexes. While the size of focal adhesions increases during senescence, it is unknown if and how this is accompanied by a remodeling of the internal focal adhesion structure. Our study uses metal-induced energy transfer to study the axial dimension of focal adhesion proteins from oxidative-stress-induced senescent cells with nanometer precision, and compares these to unstressed cells. We influenced cytoskeletal tension and the functioning of mechanosensitive ion channels using drugs and studied the combined effect of senescence and drug intervention on the focal adhesion structure. We found that H2O2-induced restructuring of the focal adhesion complex indicates a loss of tension and altered talin complexation. Mass spectroscopy-based proteomics confirmed the differential regulation of several cytoskeletal proteins induced by H2O2 treatment. |
| first_indexed | 2024-03-13T04:50:42Z |
| format | Article |
| id | doaj.art-9c01751e1b5b4967a557497b913636f1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T04:50:42Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9c01751e1b5b4967a557497b913636f12023-06-18T11:13:32ZengNature PortfolioScientific Reports2045-23222023-06-0113111710.1038/s41598-023-36347-2Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescenceCarolin Grandy0Fabian Port1Meytal Radzinski2Karmveer Singh3Dorothee Erz4Jonas Pfeil5Dana Reichmann6Kay-Eberhard Gottschalk7Institute of Experimental Physics, Ulm UniversityInstitute of Experimental Physics, Ulm UniversityAlexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, Edmond J. Safra Campus-Givat RamDepartment of Dermatology and Allergic Diseases, Ulm UniversityInstitute of Experimental Physics, Ulm UniversityInstitute of Experimental Physics, Ulm UniversityAlexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, Edmond J. Safra Campus-Givat RamInstitute of Experimental Physics, Ulm UniversityAbstract Cellular senescence is a phenotype characterized by cessation of cell division, which can be caused by exhaustive replication or environmental stress. It is involved in age-related pathophysiological conditions and affects both the cellular cytoskeleton and the prime cellular mechanosensors, focal adhesion complexes. While the size of focal adhesions increases during senescence, it is unknown if and how this is accompanied by a remodeling of the internal focal adhesion structure. Our study uses metal-induced energy transfer to study the axial dimension of focal adhesion proteins from oxidative-stress-induced senescent cells with nanometer precision, and compares these to unstressed cells. We influenced cytoskeletal tension and the functioning of mechanosensitive ion channels using drugs and studied the combined effect of senescence and drug intervention on the focal adhesion structure. We found that H2O2-induced restructuring of the focal adhesion complex indicates a loss of tension and altered talin complexation. Mass spectroscopy-based proteomics confirmed the differential regulation of several cytoskeletal proteins induced by H2O2 treatment.https://doi.org/10.1038/s41598-023-36347-2 |
| spellingShingle | Carolin Grandy Fabian Port Meytal Radzinski Karmveer Singh Dorothee Erz Jonas Pfeil Dana Reichmann Kay-Eberhard Gottschalk Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescence Scientific Reports |
| title | Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescence |
| title_full | Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescence |
| title_fullStr | Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescence |
| title_full_unstemmed | Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescence |
| title_short | Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescence |
| title_sort | remodeling of the focal adhesion complex by hydrogen peroxide induced senescence |
| url | https://doi.org/10.1038/s41598-023-36347-2 |
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