Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler Chickens
The present study aimed to assess the potential protective effects of cinnamon (Cinnamomum zeylanicum, Cin) and probiotic against CuSO<sub>4</sub>-induced nephrotoxicity in broiler chickens. One-day-old Cobb chicks were assigned into seven groups (15 birds/group): control group, fed basa...
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MDPI AG
2021-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1609 |
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| author | Sara T. Elazab Nahla S. Elshater Asmaa T. Y. Kishaway Huda A. EI-Emam |
| author_facet | Sara T. Elazab Nahla S. Elshater Asmaa T. Y. Kishaway Huda A. EI-Emam |
| author_sort | Sara T. Elazab |
| collection | DOAJ |
| description | The present study aimed to assess the potential protective effects of cinnamon (Cinnamomum zeylanicum, Cin) and probiotic against CuSO<sub>4</sub>-induced nephrotoxicity in broiler chickens. One-day-old Cobb chicks were assigned into seven groups (15 birds/group): control group, fed basal diet; Cin group, fed the basal diet mixed with Cin (200 mg/kg); PR group, receiving PR (1 g/4 L water); Cu group, fed the basal diets mixed with CuSO<sub>4</sub> (300 mg/kg); Cu + Cin group; Cu + PR group; and Cu + Cin + PR group. All treatments were given daily for 6 weeks. Treatment of Cu-intoxicated chickens with Cin and/or PR reduced (<i>p</i> < 0.05) Cu contents in renal tissues and serum levels of urea, creatinine, and uric acid compared to the Cu group. Moreover, Cin and PR treatment decreased lipid peroxidation and increased antioxidant enzyme activities in chickens’ kidney. Additionally, significant reduction (<i>p</i> < 0.05) in the mRNA expression of tumor necrosis factor alpha (TNF-α), interleukin (IL-2) and Bax, and in cyclooxygenase (COX-II) enzyme expression, and significant elevation (<i>p</i> < 0.05) in mRNA expression of IL-10 and Bcl-2 were observed in kidneys of Cu + Cin, Cu + PR, and Cu + Cin + PR groups compared to Cu group. Conclusively, Cin and/or PR afford considerable renal protection against Cu-induced nephrotoxicity in chickens. |
| first_indexed | 2024-03-10T10:54:48Z |
| format | Article |
| id | doaj.art-9c0596e3c52c4b32bca7c6b16c478b8f |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T10:54:48Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-9c0596e3c52c4b32bca7c6b16c478b8f2023-11-21T21:59:57ZengMDPI AGAnimals2076-26152021-05-01116160910.3390/ani11061609Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler ChickensSara T. Elazab0Nahla S. Elshater1Asmaa T. Y. Kishaway2Huda A. EI-Emam3Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptReference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza 12618, EgyptDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Al Sharqia Governorate 44519, EgyptDepartment of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptThe present study aimed to assess the potential protective effects of cinnamon (Cinnamomum zeylanicum, Cin) and probiotic against CuSO<sub>4</sub>-induced nephrotoxicity in broiler chickens. One-day-old Cobb chicks were assigned into seven groups (15 birds/group): control group, fed basal diet; Cin group, fed the basal diet mixed with Cin (200 mg/kg); PR group, receiving PR (1 g/4 L water); Cu group, fed the basal diets mixed with CuSO<sub>4</sub> (300 mg/kg); Cu + Cin group; Cu + PR group; and Cu + Cin + PR group. All treatments were given daily for 6 weeks. Treatment of Cu-intoxicated chickens with Cin and/or PR reduced (<i>p</i> < 0.05) Cu contents in renal tissues and serum levels of urea, creatinine, and uric acid compared to the Cu group. Moreover, Cin and PR treatment decreased lipid peroxidation and increased antioxidant enzyme activities in chickens’ kidney. Additionally, significant reduction (<i>p</i> < 0.05) in the mRNA expression of tumor necrosis factor alpha (TNF-α), interleukin (IL-2) and Bax, and in cyclooxygenase (COX-II) enzyme expression, and significant elevation (<i>p</i> < 0.05) in mRNA expression of IL-10 and Bcl-2 were observed in kidneys of Cu + Cin, Cu + PR, and Cu + Cin + PR groups compared to Cu group. Conclusively, Cin and/or PR afford considerable renal protection against Cu-induced nephrotoxicity in chickens.https://www.mdpi.com/2076-2615/11/6/1609cinnamoncopperprobioticnephrotoxicityoxidative stressinflammation |
| spellingShingle | Sara T. Elazab Nahla S. Elshater Asmaa T. Y. Kishaway Huda A. EI-Emam Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler Chickens Animals cinnamon copper probiotic nephrotoxicity oxidative stress inflammation |
| title | Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler Chickens |
| title_full | Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler Chickens |
| title_fullStr | Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler Chickens |
| title_full_unstemmed | Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler Chickens |
| title_short | Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler Chickens |
| title_sort | cinnamon extract and probiotic supplementation alleviate copper induced nephrotoxicity via modulating oxidative stress inflammation and apoptosis in broiler chickens |
| topic | cinnamon copper probiotic nephrotoxicity oxidative stress inflammation |
| url | https://www.mdpi.com/2076-2615/11/6/1609 |
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