The first transcriptome dataset of roselle (Hibiscus sabdariffa L.) calyces during maturation
Roselle (Hibiscus sabdariffa L.) is recognized for its phytochemical compounds such as anthocyanins, which possess pharmacological potentials in the treatments of hypertension, diabetes, cancer, hyperlipidaemia and hyperglycaemia. The calyx is the most commercially valuable part of the roselle and u...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340922008198 |
| _version_ | 1811206319869263872 |
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| author | Nur Atheeqah Hamzah Christina Seok Yien Yong Meenakshii Nallappan |
| author_facet | Nur Atheeqah Hamzah Christina Seok Yien Yong Meenakshii Nallappan |
| author_sort | Nur Atheeqah Hamzah |
| collection | DOAJ |
| description | Roselle (Hibiscus sabdariffa L.) is recognized for its phytochemical compounds such as anthocyanins, which possess pharmacological potentials in the treatments of hypertension, diabetes, cancer, hyperlipidaemia and hyperglycaemia. The calyx is the most commercially valuable part of the roselle and usually harvested at maturation. However, genetic study to understand the transcriptome changes in the calyx during maturation has yet to be explored. In this study, we sequenced the transcriptomes of roselle calyces at maturation stages III and IV using Illumina NextSeq 500 platform. These are the two most critical maturation stages in roselle, as these stages are often associated with the quality of the calyx. Over 200 million good quality paired-end reads were generated and de novo assembled into a reference transcriptome consisting of 221,334 transcripts with N50 score of 491bp. Among these transcripts, 92,974 transcripts (42%) were successfully annotated. The total number of significantly differentially expressed genes (DEGs) and the top five most significantly regulated genes in each of the maturation stage were presented. Twenty-one genes implicated in the biosynthesis of anthocyanins and their relative expressions in the calyx tissues at the two maturation stages were reported. Two secondary metabolites biosynthesis pathways that attained a relatively higher number of DEG mappings compared to other pathways were also reported. The findings from this work provide novel insights to better understand the transcriptional changes in roselle during calyx maturation, and the data made available here is intended for continued genetic study on roselle. The work is registered under NCBI Bioproject PRJNA664826. The raw sequencing reads are available in Short Read Archive with the accession numbers SRX9171161, SRX9171162, SRX9171163, SRX9171164, SRX9171165 and SRX9171166. |
| first_indexed | 2024-04-12T03:45:54Z |
| format | Article |
| id | doaj.art-9c060b3faf1048aa87647a77b5ae28a1 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-04-12T03:45:54Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-9c060b3faf1048aa87647a77b5ae28a12022-12-22T03:49:08ZengElsevierData in Brief2352-34092022-12-0145108613The first transcriptome dataset of roselle (Hibiscus sabdariffa L.) calyces during maturationNur Atheeqah Hamzah0Christina Seok Yien Yong1Meenakshii Nallappan2Department of Biology, Universiti Putra Malaysia, Jalan Universiti 1, 43400 UPM Serdang, Selangor Darul Ehsan, MalaysiaCorresponding author.; Department of Biology, Universiti Putra Malaysia, Jalan Universiti 1, 43400 UPM Serdang, Selangor Darul Ehsan, MalaysiaDepartment of Biology, Universiti Putra Malaysia, Jalan Universiti 1, 43400 UPM Serdang, Selangor Darul Ehsan, MalaysiaRoselle (Hibiscus sabdariffa L.) is recognized for its phytochemical compounds such as anthocyanins, which possess pharmacological potentials in the treatments of hypertension, diabetes, cancer, hyperlipidaemia and hyperglycaemia. The calyx is the most commercially valuable part of the roselle and usually harvested at maturation. However, genetic study to understand the transcriptome changes in the calyx during maturation has yet to be explored. In this study, we sequenced the transcriptomes of roselle calyces at maturation stages III and IV using Illumina NextSeq 500 platform. These are the two most critical maturation stages in roselle, as these stages are often associated with the quality of the calyx. Over 200 million good quality paired-end reads were generated and de novo assembled into a reference transcriptome consisting of 221,334 transcripts with N50 score of 491bp. Among these transcripts, 92,974 transcripts (42%) were successfully annotated. The total number of significantly differentially expressed genes (DEGs) and the top five most significantly regulated genes in each of the maturation stage were presented. Twenty-one genes implicated in the biosynthesis of anthocyanins and their relative expressions in the calyx tissues at the two maturation stages were reported. Two secondary metabolites biosynthesis pathways that attained a relatively higher number of DEG mappings compared to other pathways were also reported. The findings from this work provide novel insights to better understand the transcriptional changes in roselle during calyx maturation, and the data made available here is intended for continued genetic study on roselle. The work is registered under NCBI Bioproject PRJNA664826. The raw sequencing reads are available in Short Read Archive with the accession numbers SRX9171161, SRX9171162, SRX9171163, SRX9171164, SRX9171165 and SRX9171166.http://www.sciencedirect.com/science/article/pii/S2352340922008198AnthocyaninsPhytochemicalsDifferential gene expressionHibiscus sabdariffa var UMKLSecondary metabolitesRNA sequencing |
| spellingShingle | Nur Atheeqah Hamzah Christina Seok Yien Yong Meenakshii Nallappan The first transcriptome dataset of roselle (Hibiscus sabdariffa L.) calyces during maturation Data in Brief Anthocyanins Phytochemicals Differential gene expression Hibiscus sabdariffa var UMKL Secondary metabolites RNA sequencing |
| title | The first transcriptome dataset of roselle (Hibiscus sabdariffa L.) calyces during maturation |
| title_full | The first transcriptome dataset of roselle (Hibiscus sabdariffa L.) calyces during maturation |
| title_fullStr | The first transcriptome dataset of roselle (Hibiscus sabdariffa L.) calyces during maturation |
| title_full_unstemmed | The first transcriptome dataset of roselle (Hibiscus sabdariffa L.) calyces during maturation |
| title_short | The first transcriptome dataset of roselle (Hibiscus sabdariffa L.) calyces during maturation |
| title_sort | first transcriptome dataset of roselle hibiscus sabdariffa l calyces during maturation |
| topic | Anthocyanins Phytochemicals Differential gene expression Hibiscus sabdariffa var UMKL Secondary metabolites RNA sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2352340922008198 |
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