Cytotoxic effect of Myrtus communis, Aristolochia longa, and Calycotome spinosa on human erythrocyte cells
Introduction. Myrtus communis, Aristolochia longa, and Calycotome spinosa are medicinal plants frequently used in Algeria. Some plants can cause a fragility of the erythrocyte membrane and lead to hemolysis. Therefore, we aimed to study the cytotoxicity of aqueous extracts from the aerial part of th...
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| Format: | Article |
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Kemerovo State University
2021-11-01
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| Series: | Foods and Raw Materials |
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| Online Access: | http://jfrm.ru/en/issues/1879/1955/ |
| _version_ | 1819001547322818560 |
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| author | Leila Gadouche Azdinia Zidane Khayra Zerrouki Karima Azouni Saadia Bouinoune |
| author_facet | Leila Gadouche Azdinia Zidane Khayra Zerrouki Karima Azouni Saadia Bouinoune |
| author_sort | Leila Gadouche |
| collection | DOAJ |
| description | Introduction. Myrtus communis, Aristolochia longa, and Calycotome spinosa are medicinal plants frequently used in Algeria. Some plants can cause a fragility of the erythrocyte membrane and lead to hemolysis. Therefore, we aimed to study the cytotoxicity of aqueous extracts from the aerial part of these species against red blood cells. Study objects and methods. The hemolytic effect was determined spectrophotometrically by incubating an erythrocyte solution with different concentrations of the aqueous extracts (25, 50, 100, and 200 mg/mL) at 37°C during one hour. In addition, we performed phytochemical screening and measured the contents of polyphenols and flavonoids. Results and discussion. After one hour of incubation of human red blood cells with the aqueous extracts at different concentrations, the hemolysis percentage showed a significant leak of hemoglobin with A. longa (68.75 ± 6.11%; 200 mg/mL), the most toxic extract followed by C. spinosa (34.86 ± 5.06%; 200 mg/mL). In contrast, M. communis showed very low cytotoxicity (20.13 ± 3.11%; 200 mg/mL). Conclusion. These plants are sources of a wide range of bioactive compounds but their use in traditional medicine must be adapted to avoid any toxic effect. |
| first_indexed | 2024-12-20T22:50:57Z |
| format | Article |
| id | doaj.art-9c072a3eb4ab4c22852a56292dfa1684 |
| institution | Directory Open Access Journal |
| issn | 2308-4057 2310-9599 |
| language | English |
| last_indexed | 2024-12-20T22:50:57Z |
| publishDate | 2021-11-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Foods and Raw Materials |
| spelling | doaj.art-9c072a3eb4ab4c22852a56292dfa16842022-12-21T19:24:14ZengKemerovo State UniversityFoods and Raw Materials2308-40572310-95992021-11-019237938610.21603/2308-4057-2021-2-379-386Cytotoxic effect of Myrtus communis, Aristolochia longa, and Calycotome spinosa on human erythrocyte cellsLeila Gadouche0https://orcid.org/0000-0003-1757-1988Azdinia Zidane1https://orcid.org/0000-0003-1575-5994Khayra Zerrouki2https://orcid.org/0000-0002-0217-3138Karima Azouni3https://orcid.org/0000-0002-5662-079XSaadia Bouinoune4https://orcid.org/0000-0003-3780-0178University of Science and Technology Houari Boumediene, Bab Ezzouar, AlgeriaHassiba Benbouali University of Chlef, Chlef, AlgeriaHassiba Benbouali University of Chlef, Chlef, AlgeriaHassiba Benbouali University of Chlef, Chlef, AlgeriaHassiba Benbouali University of Chlef, Chlef, AlgeriaIntroduction. Myrtus communis, Aristolochia longa, and Calycotome spinosa are medicinal plants frequently used in Algeria. Some plants can cause a fragility of the erythrocyte membrane and lead to hemolysis. Therefore, we aimed to study the cytotoxicity of aqueous extracts from the aerial part of these species against red blood cells. Study objects and methods. The hemolytic effect was determined spectrophotometrically by incubating an erythrocyte solution with different concentrations of the aqueous extracts (25, 50, 100, and 200 mg/mL) at 37°C during one hour. In addition, we performed phytochemical screening and measured the contents of polyphenols and flavonoids. Results and discussion. After one hour of incubation of human red blood cells with the aqueous extracts at different concentrations, the hemolysis percentage showed a significant leak of hemoglobin with A. longa (68.75 ± 6.11%; 200 mg/mL), the most toxic extract followed by C. spinosa (34.86 ± 5.06%; 200 mg/mL). In contrast, M. communis showed very low cytotoxicity (20.13 ± 3.11%; 200 mg/mL). Conclusion. These plants are sources of a wide range of bioactive compounds but their use in traditional medicine must be adapted to avoid any toxic effect.http://jfrm.ru/en/issues/1879/1955/myrtus communisaristolochia longacalycotome spinosafolk medicinephenolic compoundsalkaloidshemoglobincell toxicityhemolytic activity |
| spellingShingle | Leila Gadouche Azdinia Zidane Khayra Zerrouki Karima Azouni Saadia Bouinoune Cytotoxic effect of Myrtus communis, Aristolochia longa, and Calycotome spinosa on human erythrocyte cells Foods and Raw Materials myrtus communis aristolochia longa calycotome spinosa folk medicine phenolic compounds alkaloids hemoglobin cell toxicity hemolytic activity |
| title | Cytotoxic effect of Myrtus communis, Aristolochia longa, and Calycotome spinosa on human erythrocyte cells |
| title_full | Cytotoxic effect of Myrtus communis, Aristolochia longa, and Calycotome spinosa on human erythrocyte cells |
| title_fullStr | Cytotoxic effect of Myrtus communis, Aristolochia longa, and Calycotome spinosa on human erythrocyte cells |
| title_full_unstemmed | Cytotoxic effect of Myrtus communis, Aristolochia longa, and Calycotome spinosa on human erythrocyte cells |
| title_short | Cytotoxic effect of Myrtus communis, Aristolochia longa, and Calycotome spinosa on human erythrocyte cells |
| title_sort | cytotoxic effect of myrtus communis aristolochia longa and calycotome spinosa on human erythrocyte cells |
| topic | myrtus communis aristolochia longa calycotome spinosa folk medicine phenolic compounds alkaloids hemoglobin cell toxicity hemolytic activity |
| url | http://jfrm.ru/en/issues/1879/1955/ |
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