| Summary: | Tryptantrin and
indirubin are pharmacologically active compounds used in treatment of disseases
such as cancer and Alzheimer's. In this study, we investigated the influences
of different concentrations of methyl jasmonate (MeJa) and putrescine (Put) on
tryptanthrin and indirubin production in leaf explants and development of Isatis demiriziana Mısırdalı grown in
vitro. In all media treated with methyl jasmonate, tryptanthrin production in
leaves of plantlets showed an increase. The highest increase in tryptanthrin
production was observed in solid Murashige-Skoog (MS) medium containing 1.0 mM
MeJa (154.026 ± 0.11 µg g-1), about 2.85-fold higher than the control
(untreated plantlets) (40.017 ± 0.031 µg g-1). Production of tryptanthrin
decreased about 2.56-fold in the leaves of plantlets treated with Put, when
compared to control. The highest indirubin production was obtained in the leaves
of plantlets grown in the MS medium containing 0.1 mM MeJa (11.274 ± 0.035 µg
g-1) but treatments with Put didn’t show any positive affect on the indirubin
production. Analysis of tryptanthrin and indirubin were performed using high
performance liquid chromatography (HPLC).
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