Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı
Tryptantrin and indirubin are pharmacologically active compounds used in treatment of disseases such as cancer and Alzheimer's. In this study, we investigated the influences of different concentrations of methyl jasmonate (MeJa) and putrescine (Put) on tryptanthrin and indirubin production in l...
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| Format: | Article |
| Language: | English |
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International Journal of Secondary Metabolite
2019-10-01
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| Series: | International Journal of Secondary Metabolite |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/ijsm/issue/47863/521498?publisher=ijate |
| _version_ | 1797906182507069440 |
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| author | Özgür Karakaş |
| author_facet | Özgür Karakaş |
| author_sort | Özgür Karakaş |
| collection | DOAJ |
| description | Tryptantrin and
indirubin are pharmacologically active compounds used in treatment of disseases
such as cancer and Alzheimer's. In this study, we investigated the influences
of different concentrations of methyl jasmonate (MeJa) and putrescine (Put) on
tryptanthrin and indirubin production in leaf explants and development of Isatis demiriziana Mısırdalı grown in
vitro. In all media treated with methyl jasmonate, tryptanthrin production in
leaves of plantlets showed an increase. The highest increase in tryptanthrin
production was observed in solid Murashige-Skoog (MS) medium containing 1.0 mM
MeJa (154.026 ± 0.11 µg g-1), about 2.85-fold higher than the control
(untreated plantlets) (40.017 ± 0.031 µg g-1). Production of tryptanthrin
decreased about 2.56-fold in the leaves of plantlets treated with Put, when
compared to control. The highest indirubin production was obtained in the leaves
of plantlets grown in the MS medium containing 0.1 mM MeJa (11.274 ± 0.035 µg
g-1) but treatments with Put didn’t show any positive affect on the indirubin
production. Analysis of tryptanthrin and indirubin were performed using high
performance liquid chromatography (HPLC). |
| first_indexed | 2024-04-10T10:16:59Z |
| format | Article |
| id | doaj.art-9c091981784d41a3bf7ffbe785c53925 |
| institution | Directory Open Access Journal |
| issn | 2148-6905 |
| language | English |
| last_indexed | 2024-04-10T10:16:59Z |
| publishDate | 2019-10-01 |
| publisher | International Journal of Secondary Metabolite |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj.art-9c091981784d41a3bf7ffbe785c539252023-02-15T16:21:50ZengInternational Journal of Secondary MetaboliteInternational Journal of Secondary Metabolite2148-69052019-10-016324125010.21448/ijsm.521498618Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana MısırdalıÖzgür KarakaşTryptantrin and indirubin are pharmacologically active compounds used in treatment of disseases such as cancer and Alzheimer's. In this study, we investigated the influences of different concentrations of methyl jasmonate (MeJa) and putrescine (Put) on tryptanthrin and indirubin production in leaf explants and development of Isatis demiriziana Mısırdalı grown in vitro. In all media treated with methyl jasmonate, tryptanthrin production in leaves of plantlets showed an increase. The highest increase in tryptanthrin production was observed in solid Murashige-Skoog (MS) medium containing 1.0 mM MeJa (154.026 ± 0.11 µg g-1), about 2.85-fold higher than the control (untreated plantlets) (40.017 ± 0.031 µg g-1). Production of tryptanthrin decreased about 2.56-fold in the leaves of plantlets treated with Put, when compared to control. The highest indirubin production was obtained in the leaves of plantlets grown in the MS medium containing 0.1 mM MeJa (11.274 ± 0.035 µg g-1) but treatments with Put didn’t show any positive affect on the indirubin production. Analysis of tryptanthrin and indirubin were performed using high performance liquid chromatography (HPLC).https://dergipark.org.tr/tr/pub/ijsm/issue/47863/521498?publisher=ijatetryptanthrinindirubinmethyl jasmonateputrescineisatis demiriziana |
| spellingShingle | Özgür Karakaş Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı International Journal of Secondary Metabolite tryptanthrin indirubin methyl jasmonate putrescine isatis demiriziana |
| title | Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı |
| title_full | Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı |
| title_fullStr | Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı |
| title_full_unstemmed | Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı |
| title_short | Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı |
| title_sort | effects of methyl jasmonate and putrescine on tryptanthrin and indirubin production in in vitro cultures of isatis demiriziana misirdali |
| topic | tryptanthrin indirubin methyl jasmonate putrescine isatis demiriziana |
| url | https://dergipark.org.tr/tr/pub/ijsm/issue/47863/521498?publisher=ijate |
| work_keys_str_mv | AT ozgurkarakas effectsofmethyljasmonateandputrescineontryptanthrinandindirubinproductionininvitroculturesofisatisdemirizianamısırdalı |