Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor
Objective(s)Rapid tests for detection of Streptococcus agalactiae or Group B Streptococci (GBS) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. This study aimed to evaluate the PCR assays targeting the 16S ribosomal RNA gene (16S rDNA) for detection of the GBS in...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2010-09-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://www.mums.ac.ir/shares/basic_medical/basicmedjou/89/fall/a4.pdf |
| _version_ | 1818474088463597568 |
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| author | Farnaz Fatemi Parviz Pakzad Hojjat Zeraati Saeed Talebi Soheila Asgari Mohammad-Mehdi Akhondi Leili Chamani-Tabriz |
| author_facet | Farnaz Fatemi Parviz Pakzad Hojjat Zeraati Saeed Talebi Soheila Asgari Mohammad-Mehdi Akhondi Leili Chamani-Tabriz |
| author_sort | Farnaz Fatemi |
| collection | DOAJ |
| description | Objective(s)Rapid tests for detection of Streptococcus agalactiae or Group B Streptococci (GBS) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. This study aimed to evaluate the PCR assays targeting the 16S ribosomal RNA gene (16S rDNA) for detection of the GBS in comparison with a specific culture method. Materials and MethodsTwo swabs were used to obtain vaginal specimens from the 330 pregnant women attended delivery room at Hedayat hospital, Tehran, Iran. One swab was analyzed by direct plating onto selective GBS agar medium (ISLAM) and the other swab was used for a PCR assay, which amplified the 16S rDNA of S. agalactiae. Comparative study between the selective culture and the PCR assay was done among the 330 tested women.ResultsThe GBS colonization rate based on the culture results was 20.6% (68/330). Both culture and PCR methods were positive for 56 and negative for 253 women. The culture method was positive and PCR was negative in 12 women. The culture was negative and the PCR positive for 9 women. Sensitivity of the PCR assay was 82.3% and specificity was 96.5%. The positive predictive value was 86.15% and negative predictive value was 95.4%. ConclusionISLAM diagnostic procedure and PCR are rapid and reliable analyzing methods, which might be useful for accurate diagnosis of GBS colonization in pregnant women at the time of delivery. |
| first_indexed | 2024-04-14T04:31:55Z |
| format | Article |
| id | doaj.art-9c094d44a951475d804e0a2d94fe8e21 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-14T04:31:55Z |
| publishDate | 2010-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-9c094d44a951475d804e0a2d94fe8e212022-12-22T02:12:01ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742010-09-011347Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during LaborFarnaz FatemiParviz PakzadHojjat ZeraatiSaeed TalebiSoheila AsgariMohammad-Mehdi AkhondiLeili Chamani-TabrizObjective(s)Rapid tests for detection of Streptococcus agalactiae or Group B Streptococci (GBS) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. This study aimed to evaluate the PCR assays targeting the 16S ribosomal RNA gene (16S rDNA) for detection of the GBS in comparison with a specific culture method. Materials and MethodsTwo swabs were used to obtain vaginal specimens from the 330 pregnant women attended delivery room at Hedayat hospital, Tehran, Iran. One swab was analyzed by direct plating onto selective GBS agar medium (ISLAM) and the other swab was used for a PCR assay, which amplified the 16S rDNA of S. agalactiae. Comparative study between the selective culture and the PCR assay was done among the 330 tested women.ResultsThe GBS colonization rate based on the culture results was 20.6% (68/330). Both culture and PCR methods were positive for 56 and negative for 253 women. The culture method was positive and PCR was negative in 12 women. The culture was negative and the PCR positive for 9 women. Sensitivity of the PCR assay was 82.3% and specificity was 96.5%. The positive predictive value was 86.15% and negative predictive value was 95.4%. ConclusionISLAM diagnostic procedure and PCR are rapid and reliable analyzing methods, which might be useful for accurate diagnosis of GBS colonization in pregnant women at the time of delivery.http://www.mums.ac.ir/shares/basic_medical/basicmedjou/89/fall/a4.pdfKeywords: Early diagnosisPCRrRNA geneStreptococcus agalactiaeStreptococcal infection |
| spellingShingle | Farnaz Fatemi Parviz Pakzad Hojjat Zeraati Saeed Talebi Soheila Asgari Mohammad-Mehdi Akhondi Leili Chamani-Tabriz Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor Iranian Journal of Basic Medical Sciences Keywords: Early diagnosis PCR rRNA gene Streptococcus agalactiae Streptococcal infection |
| title | Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor |
| title_full | Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor |
| title_fullStr | Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor |
| title_full_unstemmed | Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor |
| title_short | Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor |
| title_sort | comparative molecular and microbiologic diagnosis of vaginal colonization by group b streptococcus in pregnant women during labor |
| topic | Keywords: Early diagnosis PCR rRNA gene Streptococcus agalactiae Streptococcal infection |
| url | http://www.mums.ac.ir/shares/basic_medical/basicmedjou/89/fall/a4.pdf |
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