Induction of mitochondrial apoptotic pathway by raloxifene and estrogen in human endometrial stromal ThESC cell line
Introduction: Endometrial hyperplasia is a condition that occurs as a result of hormonal imbalance between estrogen and progesterone. Morphological disturbance of endometrial cells occurs consequently leading towards endometrial cancer. In therapy of endometrial hyperplasia SERMs are used to supres...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2016-05-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.termedia.pl/Induction-of-mitochondrial-apoptotic-pathway-by-raloxifene-and-estrogen-in-human-endometrial-stromal-ThESC-cell-line,19,27550,1,1.html |
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| author | Ivana Nikolic Marija Andjelkovic Milan Zaric Ivanka Zelen Petar Canovic Zoran Milosavljevic Marina Mitrovic |
| author_facet | Ivana Nikolic Marija Andjelkovic Milan Zaric Ivanka Zelen Petar Canovic Zoran Milosavljevic Marina Mitrovic |
| author_sort | Ivana Nikolic |
| collection | DOAJ |
| description | Introduction: Endometrial hyperplasia is a condition that occurs as a result of hormonal imbalance between estrogen and progesterone. Morphological disturbance of endometrial cells occurs consequently leading towards endometrial cancer. In therapy of endometrial hyperplasia SERMs are used to supress effects of locally high estrogen level in uterus. There is strong evidence suggesting that estrogen could be involved in cell death – apoptosis. There are no experimental data demstrating the direct apoptotic effect of both raloxifene and estrogen on the ThESC cell line. The aim of our study wa sto investigate both cytotoxic and apototic mechanism of raloxifene and estrogen – induced death in the ThESC cell line.
Material and methods : In order to determine their cytotoxic and apoptotic effects, various doses of raloxifene and estrogen were applied to the ThESC cell line for 24h. After the treatment MTT assay, FACS analysis and immunofluoroscence method were conducted.
Results : The results of this study for the first time demonstrated the cytotoxic and apoptotic effects of raloxifene and estrogen on human endometrial stromal cell line suggesting the involvement of the inner, mitochondrial apoptotic pathway.
Conclusions : Our results demonstrated apoptotic effects of investigated drugs in the ThESC cell line through increasing the Bax/Bcl-2 ratio and activation of caspase 3. |
| first_indexed | 2024-12-10T17:48:37Z |
| format | Article |
| id | doaj.art-9c0a6063f635427492e41e04ab7e0cb4 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-12-10T17:48:37Z |
| publishDate | 2016-05-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-9c0a6063f635427492e41e04ab7e0cb42022-12-22T01:39:09ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512016-05-0113229330110.5114/aoms.2016.5987427550Induction of mitochondrial apoptotic pathway by raloxifene and estrogen in human endometrial stromal ThESC cell lineIvana NikolicMarija AndjelkovicMilan ZaricIvanka ZelenPetar CanovicZoran MilosavljevicMarina MitrovicIntroduction: Endometrial hyperplasia is a condition that occurs as a result of hormonal imbalance between estrogen and progesterone. Morphological disturbance of endometrial cells occurs consequently leading towards endometrial cancer. In therapy of endometrial hyperplasia SERMs are used to supress effects of locally high estrogen level in uterus. There is strong evidence suggesting that estrogen could be involved in cell death – apoptosis. There are no experimental data demstrating the direct apoptotic effect of both raloxifene and estrogen on the ThESC cell line. The aim of our study wa sto investigate both cytotoxic and apototic mechanism of raloxifene and estrogen – induced death in the ThESC cell line. Material and methods : In order to determine their cytotoxic and apoptotic effects, various doses of raloxifene and estrogen were applied to the ThESC cell line for 24h. After the treatment MTT assay, FACS analysis and immunofluoroscence method were conducted. Results : The results of this study for the first time demonstrated the cytotoxic and apoptotic effects of raloxifene and estrogen on human endometrial stromal cell line suggesting the involvement of the inner, mitochondrial apoptotic pathway. Conclusions : Our results demonstrated apoptotic effects of investigated drugs in the ThESC cell line through increasing the Bax/Bcl-2 ratio and activation of caspase 3.https://www.termedia.pl/Induction-of-mitochondrial-apoptotic-pathway-by-raloxifene-and-estrogen-in-human-endometrial-stromal-ThESC-cell-line,19,27550,1,1.htmlapoptosis raloxifene estrogen endometrial hyperplasia inner apoptotic pathway |
| spellingShingle | Ivana Nikolic Marija Andjelkovic Milan Zaric Ivanka Zelen Petar Canovic Zoran Milosavljevic Marina Mitrovic Induction of mitochondrial apoptotic pathway by raloxifene and estrogen in human endometrial stromal ThESC cell line Archives of Medical Science apoptosis raloxifene estrogen endometrial hyperplasia inner apoptotic pathway |
| title | Induction of mitochondrial apoptotic pathway by raloxifene and estrogen in human endometrial stromal ThESC cell line |
| title_full | Induction of mitochondrial apoptotic pathway by raloxifene and estrogen in human endometrial stromal ThESC cell line |
| title_fullStr | Induction of mitochondrial apoptotic pathway by raloxifene and estrogen in human endometrial stromal ThESC cell line |
| title_full_unstemmed | Induction of mitochondrial apoptotic pathway by raloxifene and estrogen in human endometrial stromal ThESC cell line |
| title_short | Induction of mitochondrial apoptotic pathway by raloxifene and estrogen in human endometrial stromal ThESC cell line |
| title_sort | induction of mitochondrial apoptotic pathway by raloxifene and estrogen in human endometrial stromal thesc cell line |
| topic | apoptosis raloxifene estrogen endometrial hyperplasia inner apoptotic pathway |
| url | https://www.termedia.pl/Induction-of-mitochondrial-apoptotic-pathway-by-raloxifene-and-estrogen-in-human-endometrial-stromal-ThESC-cell-line,19,27550,1,1.html |
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