Genetic Variants of Intron Region of Aquaporin AQP5 Gene and Development of Pulmonary Edema in Lung Infection Complicated by Septic Shock
Purpose of the study. Determine the value of genetic variants of a single nucleotide polymorphic site rs3736309 of intron 3 of aquaporin5 (AQP5) gene in the course of critical illness in patients with documented pulmonary infection. Materials and methods. Patients with critical illness admitted to t...
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| Format: | Article |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2016-07-01
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| Series: | Общая реаниматология |
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| Online Access: | https://www.reanimatology.com/rmt/article/view/1526 |
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| author | A. E. Myazin A. G. Chumachenko A. N. Kuzovlev A. M. Golubev V. V. Moroz A. M. Gaponov A. V. Tutelian M. A. Golubev V. M. Pisarev |
| author_facet | A. E. Myazin A. G. Chumachenko A. N. Kuzovlev A. M. Golubev V. V. Moroz A. M. Gaponov A. V. Tutelian M. A. Golubev V. M. Pisarev |
| author_sort | A. E. Myazin |
| collection | DOAJ |
| description | Purpose of the study. Determine the value of genetic variants of a single nucleotide polymorphic site rs3736309 of intron 3 of aquaporin5 (AQP5) gene in the course of critical illness in patients with documented pulmonary infection. Materials and methods. Patients with critical illness admitted to the intensive care units were examined during the course of treatment (n=86, age 27 to 82 years, mean age 53.20±14.34 years). Main diagnosis included malignancies (15%), peritonitis (16%) and necrotizing pancreatitis (37%). Patients developed nosocomial pneumonia (55%), acute respiratory distress syndrome (ARDS) (54%), septic shock (48%), ARDS combined with septic shock (33%). Bacterial species of Pseudomonas aeruginosa , Klebsiella pneumoniae, Acinetobacter baumannii, and/or Proteus mirabilis alone or in association were revealed in lavage fluid. DNA genotyping DNA was carried out using tetraprimer polymerase chain reaction (PCR). Statistical processing was performed using GraphPad InStat program (GraphPad, USA).Results. The distribution of frequencies of genotypes AA, GA and GG (AQP5, rs3736309) in cohort of patients corresponded to HardyWeinberg equilibrium (P=0.923) and was similar to frequencies of same alleles determined in a conditionally healthy Caucasian individuals (literature data) (P>0.05). In a subgroup of patients with septic shock and AQP5 AA (rs3736309) genotype the lower EVLWI values were found compared to patients with genotypes GG and GA with septic shock in spite of the same approach to treatment. The differences between genetically different subgroups of patients with septic shock were maintained throughout the life of the survey (P<0.05,days 1, 3, 5 and 7). Genetic variant AQP5 G+ (rs3736309) contributed to the development of pulmonary edema resistant to treatment (odds ratio, OR = 6,75; P=0.032). Only the subgroup of patients with septic shock and genotype G + (but not all patients or the subgroup of patients without septic shock of the same genotype) were characterized by significantly elevated levels of surfactant protein SPD in plasma compared to patients of genotype AQP5 AA with septic shock (P<0.05).Conclusion. In septic shock, the presence of homozygous variant allele A (AA) of AQP5 rs3736309 is a favorable factor for patients developing the pulmonary edema. The presence of allele AQP5 G (rs3736309) is a risk factor for developing severe pulmonary edema and unfavorable prognosis in spite of treatment. |
| first_indexed | 2024-04-10T01:28:30Z |
| format | Article |
| id | doaj.art-9c127fe0b9b84988969339fc62b07635 |
| institution | Directory Open Access Journal |
| issn | 1813-9779 2411-7110 |
| language | English |
| last_indexed | 2024-04-10T01:28:30Z |
| publishDate | 2016-07-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj.art-9c127fe0b9b84988969339fc62b076352023-03-13T09:32:54ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102016-07-0112382310.15360/1813-9779-2016-3-8-231504Genetic Variants of Intron Region of Aquaporin AQP5 Gene and Development of Pulmonary Edema in Lung Infection Complicated by Septic ShockA. E. Myazin0A. G. Chumachenko1A. N. Kuzovlev2A. M. Golubev3V. V. Moroz4A. M. Gaponov5A. V. Tutelian6M. A. Golubev7V. M. Pisarev8НИИ общей реаниматологии им. В. А. Неговского; Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии им. Д. Рогачева Минздрава РоссииНИИ общей реаниматологии им. В. А. Неговского; Центральный НИИ эпидемиологии РоспотребнадзораНИИ общей реаниматологии им. В. А. НеговскогоНИИ общей реаниматологии им. В. А. НеговскогоНИИ общей реаниматологии им. В. А. НеговскогоНИИ общей реаниматологии им. В. А. Неговского; Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии им. Д. Рогачева Минздрава РоссииФедеральный научно-клинический центр детской гематологии, онкологии и иммунологии им. Д. Рогачева Минздрава России; Центральный НИИ эпидемиологии РоспотребнадзораООО «Корпорация «Медицинские электронные данные»НИИ общей реаниматологии им. В. А. Неговского Федеральный научно-клинический центр детской гематологии, онкологии и иммунологии им. Д. Рогачева Минздрава России; Центральный НИИ эпидемиологии Роспотребнадзора; Университет штата НебраскаPurpose of the study. Determine the value of genetic variants of a single nucleotide polymorphic site rs3736309 of intron 3 of aquaporin5 (AQP5) gene in the course of critical illness in patients with documented pulmonary infection. Materials and methods. Patients with critical illness admitted to the intensive care units were examined during the course of treatment (n=86, age 27 to 82 years, mean age 53.20±14.34 years). Main diagnosis included malignancies (15%), peritonitis (16%) and necrotizing pancreatitis (37%). Patients developed nosocomial pneumonia (55%), acute respiratory distress syndrome (ARDS) (54%), septic shock (48%), ARDS combined with septic shock (33%). Bacterial species of Pseudomonas aeruginosa , Klebsiella pneumoniae, Acinetobacter baumannii, and/or Proteus mirabilis alone or in association were revealed in lavage fluid. DNA genotyping DNA was carried out using tetraprimer polymerase chain reaction (PCR). Statistical processing was performed using GraphPad InStat program (GraphPad, USA).Results. The distribution of frequencies of genotypes AA, GA and GG (AQP5, rs3736309) in cohort of patients corresponded to HardyWeinberg equilibrium (P=0.923) and was similar to frequencies of same alleles determined in a conditionally healthy Caucasian individuals (literature data) (P>0.05). In a subgroup of patients with septic shock and AQP5 AA (rs3736309) genotype the lower EVLWI values were found compared to patients with genotypes GG and GA with septic shock in spite of the same approach to treatment. The differences between genetically different subgroups of patients with septic shock were maintained throughout the life of the survey (P<0.05,days 1, 3, 5 and 7). Genetic variant AQP5 G+ (rs3736309) contributed to the development of pulmonary edema resistant to treatment (odds ratio, OR = 6,75; P=0.032). Only the subgroup of patients with septic shock and genotype G + (but not all patients or the subgroup of patients without septic shock of the same genotype) were characterized by significantly elevated levels of surfactant protein SPD in plasma compared to patients of genotype AQP5 AA with septic shock (P<0.05).Conclusion. In septic shock, the presence of homozygous variant allele A (AA) of AQP5 rs3736309 is a favorable factor for patients developing the pulmonary edema. The presence of allele AQP5 G (rs3736309) is a risk factor for developing severe pulmonary edema and unfavorable prognosis in spite of treatment.https://www.reanimatology.com/rmt/article/view/1526snpполиморфизм aqp5аквапориныполиморфизм геновотек легкихсептический шок |
| spellingShingle | A. E. Myazin A. G. Chumachenko A. N. Kuzovlev A. M. Golubev V. V. Moroz A. M. Gaponov A. V. Tutelian M. A. Golubev V. M. Pisarev Genetic Variants of Intron Region of Aquaporin AQP5 Gene and Development of Pulmonary Edema in Lung Infection Complicated by Septic Shock Общая реаниматология snp полиморфизм aqp5 аквапорины полиморфизм генов отек легких септический шок |
| title | Genetic Variants of Intron Region of Aquaporin AQP5 Gene and Development of Pulmonary Edema in Lung Infection Complicated by Septic Shock |
| title_full | Genetic Variants of Intron Region of Aquaporin AQP5 Gene and Development of Pulmonary Edema in Lung Infection Complicated by Septic Shock |
| title_fullStr | Genetic Variants of Intron Region of Aquaporin AQP5 Gene and Development of Pulmonary Edema in Lung Infection Complicated by Septic Shock |
| title_full_unstemmed | Genetic Variants of Intron Region of Aquaporin AQP5 Gene and Development of Pulmonary Edema in Lung Infection Complicated by Septic Shock |
| title_short | Genetic Variants of Intron Region of Aquaporin AQP5 Gene and Development of Pulmonary Edema in Lung Infection Complicated by Septic Shock |
| title_sort | genetic variants of intron region of aquaporin aqp5 gene and development of pulmonary edema in lung infection complicated by septic shock |
| topic | snp полиморфизм aqp5 аквапорины полиморфизм генов отек легких септический шок |
| url | https://www.reanimatology.com/rmt/article/view/1526 |
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