Identification of serum proteins AHSG, FGA and APOA-I as diagnostic biomarkers for gastric cancer
Abstract Background The development of clinically accessible biomarkers is critical for the early diagnosis of gastric cancer (GC) in patients. High-throughput proteomics techniques could not only effectively generate a serum peptide profile but also provide a new approach to identify potentially di...
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Clinical Proteomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12014-018-9194-0 |
| _version_ | 1818271914243653632 |
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| author | Feiyu Shi Hong Wu Kai Qu Qi Sun Fanni Li Chengxin Shi Yaguang Li Xiaofan Xiong Qian Qin Tianyu Yu Xin Jin Liang Cheng Qingxia Wei Yingchao Li Junjun She |
| author_facet | Feiyu Shi Hong Wu Kai Qu Qi Sun Fanni Li Chengxin Shi Yaguang Li Xiaofan Xiong Qian Qin Tianyu Yu Xin Jin Liang Cheng Qingxia Wei Yingchao Li Junjun She |
| author_sort | Feiyu Shi |
| collection | DOAJ |
| description | Abstract Background The development of clinically accessible biomarkers is critical for the early diagnosis of gastric cancer (GC) in patients. High-throughput proteomics techniques could not only effectively generate a serum peptide profile but also provide a new approach to identify potentially diagnostic and prognostic biomarkers for cancer patients. Methods In this study, we aim to identify potentially discriminating serum biomarkers for GC. In the discovery cohort, we screened potential biomarkers using magnetic-bead-based purification and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in 64 samples from 32 GC patients that were taken both pre- and post-operatively and 30 healthy volunteers that served as controls. In the validation cohort, the expression patterns and diagnostic values of serum FGA, AHSG and APOA-I were further confirmed by ELISA in 42 paired GC patients (pre- and post-operative samples from 16 patients with pathologic stage I/II and 26 with stage III/IV), 30 colorectal cancer patients, 30 hepatocellular carcinoma patients, and 28 healthy volunteers. Results ClinProTools software was used and annotated 107 peptides, 12 of which were differentially expressed among three groups (P < 0.0001, fold > 1.5). These 12 peptide peaks were further identified as FGA, AHSG, APOA-I, HBB, TXNRD1, GSPT2 and CAKP5. ELISA data suggested that the serum levels of FGA, AHSG and APOA-I in GC patients were significantly different compared with healthy controls and had favorable diagnostic values for GC patients. Moreover, we found that the serum levels of these three proteins were associated with TNM stages and could reflect tumor burden. Conclusion Our findings suggested that FGA, AHSG and APOA-I might be potential serum biomarkers for GC diagnosis. |
| first_indexed | 2024-12-12T21:33:44Z |
| format | Article |
| id | doaj.art-9c1465585d80497eb9e734136c6cda93 |
| institution | Directory Open Access Journal |
| issn | 1542-6416 1559-0275 |
| language | English |
| last_indexed | 2024-12-12T21:33:44Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical Proteomics |
| spelling | doaj.art-9c1465585d80497eb9e734136c6cda932022-12-22T00:11:15ZengBMCClinical Proteomics1542-64161559-02752018-04-0115111310.1186/s12014-018-9194-0Identification of serum proteins AHSG, FGA and APOA-I as diagnostic biomarkers for gastric cancerFeiyu Shi0Hong Wu1Kai Qu2Qi Sun3Fanni Li4Chengxin Shi5Yaguang Li6Xiaofan Xiong7Qian Qin8Tianyu Yu9Xin Jin10Liang Cheng11Qingxia Wei12Yingchao Li13Junjun She14Department of General Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of Talent Highland, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of Cell Biology and Genetics, School of Basic Medical Sciences, Xi’an Jiao Tong University Health Science CenterDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of Developmental and Stem Cell Biology, Hospital for Sick Children, University of TorontoDepartment of Gastroenterology, The First Affiliated Hospital of Xi’an Jiao Tong UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Jiao Tong UniversityAbstract Background The development of clinically accessible biomarkers is critical for the early diagnosis of gastric cancer (GC) in patients. High-throughput proteomics techniques could not only effectively generate a serum peptide profile but also provide a new approach to identify potentially diagnostic and prognostic biomarkers for cancer patients. Methods In this study, we aim to identify potentially discriminating serum biomarkers for GC. In the discovery cohort, we screened potential biomarkers using magnetic-bead-based purification and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in 64 samples from 32 GC patients that were taken both pre- and post-operatively and 30 healthy volunteers that served as controls. In the validation cohort, the expression patterns and diagnostic values of serum FGA, AHSG and APOA-I were further confirmed by ELISA in 42 paired GC patients (pre- and post-operative samples from 16 patients with pathologic stage I/II and 26 with stage III/IV), 30 colorectal cancer patients, 30 hepatocellular carcinoma patients, and 28 healthy volunteers. Results ClinProTools software was used and annotated 107 peptides, 12 of which were differentially expressed among three groups (P < 0.0001, fold > 1.5). These 12 peptide peaks were further identified as FGA, AHSG, APOA-I, HBB, TXNRD1, GSPT2 and CAKP5. ELISA data suggested that the serum levels of FGA, AHSG and APOA-I in GC patients were significantly different compared with healthy controls and had favorable diagnostic values for GC patients. Moreover, we found that the serum levels of these three proteins were associated with TNM stages and could reflect tumor burden. Conclusion Our findings suggested that FGA, AHSG and APOA-I might be potential serum biomarkers for GC diagnosis.http://link.springer.com/article/10.1186/s12014-018-9194-0Gastric cancerBiomarkerAPOA-IAHSGFGA |
| spellingShingle | Feiyu Shi Hong Wu Kai Qu Qi Sun Fanni Li Chengxin Shi Yaguang Li Xiaofan Xiong Qian Qin Tianyu Yu Xin Jin Liang Cheng Qingxia Wei Yingchao Li Junjun She Identification of serum proteins AHSG, FGA and APOA-I as diagnostic biomarkers for gastric cancer Clinical Proteomics Gastric cancer Biomarker APOA-I AHSG FGA |
| title | Identification of serum proteins AHSG, FGA and APOA-I as diagnostic biomarkers for gastric cancer |
| title_full | Identification of serum proteins AHSG, FGA and APOA-I as diagnostic biomarkers for gastric cancer |
| title_fullStr | Identification of serum proteins AHSG, FGA and APOA-I as diagnostic biomarkers for gastric cancer |
| title_full_unstemmed | Identification of serum proteins AHSG, FGA and APOA-I as diagnostic biomarkers for gastric cancer |
| title_short | Identification of serum proteins AHSG, FGA and APOA-I as diagnostic biomarkers for gastric cancer |
| title_sort | identification of serum proteins ahsg fga and apoa i as diagnostic biomarkers for gastric cancer |
| topic | Gastric cancer Biomarker APOA-I AHSG FGA |
| url | http://link.springer.com/article/10.1186/s12014-018-9194-0 |
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