Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1.
Dengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed for the detection of dengue virus NS1. A series of amino acid-subst...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0222144 |
| _version_ | 1819147295042568192 |
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| author | Ji Hong Kim Chae Hwan Cho Myung Yi Ryu Jong-Gil Kim Sei-Jung Lee Tae Jung Park Jong Pil Park |
| author_facet | Ji Hong Kim Chae Hwan Cho Myung Yi Ryu Jong-Gil Kim Sei-Jung Lee Tae Jung Park Jong Pil Park |
| author_sort | Ji Hong Kim |
| collection | DOAJ |
| description | Dengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed for the detection of dengue virus NS1. A series of amino acid-substituted synthetic peptides was rationally designed, chemically synthesized and covalently immobilized to a gold sensor surface. The sensor performance was monitored via square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Potential affinity peptides specific for NS1 were chosen according to the dynamic current decrease in SWV experiments. Using circular dichroism, the molar ellipticity of peptides (DGV BP1-BP5) was determined, indicating that they had a mostly similar in random coil structure, not totally identical. Using SWV, DGV BP1 was selected as a promising recognition peptide and limit of detection for NS1 was found to be 1.49 μg/mL by the 3-sigma rule. DGV BP1 showed good specificity and stability for NS1, with low signal interference. The validation of the sensor to detect NS1 proteins was confirmed with four dengue virus culture broth (from serotype 1 to 4) as proof-of-concept. The detection performance of our sensor incorporating DGV BP1 peptides showed a statistically significant difference. These results indicate that this strategy can potentially be used to detect the dengue virus antigen, NS1, and to diagnosis dengue fever within a miniaturized portable device in point-of-care testing. |
| first_indexed | 2024-12-22T13:27:33Z |
| format | Article |
| id | doaj.art-9c192c5c057c4d838a49fa0ee0d484b3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T13:27:33Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9c192c5c057c4d838a49fa0ee0d484b32022-12-21T18:24:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01149e022214410.1371/journal.pone.0222144Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1.Ji Hong KimChae Hwan ChoMyung Yi RyuJong-Gil KimSei-Jung LeeTae Jung ParkJong Pil ParkDengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed for the detection of dengue virus NS1. A series of amino acid-substituted synthetic peptides was rationally designed, chemically synthesized and covalently immobilized to a gold sensor surface. The sensor performance was monitored via square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Potential affinity peptides specific for NS1 were chosen according to the dynamic current decrease in SWV experiments. Using circular dichroism, the molar ellipticity of peptides (DGV BP1-BP5) was determined, indicating that they had a mostly similar in random coil structure, not totally identical. Using SWV, DGV BP1 was selected as a promising recognition peptide and limit of detection for NS1 was found to be 1.49 μg/mL by the 3-sigma rule. DGV BP1 showed good specificity and stability for NS1, with low signal interference. The validation of the sensor to detect NS1 proteins was confirmed with four dengue virus culture broth (from serotype 1 to 4) as proof-of-concept. The detection performance of our sensor incorporating DGV BP1 peptides showed a statistically significant difference. These results indicate that this strategy can potentially be used to detect the dengue virus antigen, NS1, and to diagnosis dengue fever within a miniaturized portable device in point-of-care testing.https://doi.org/10.1371/journal.pone.0222144 |
| spellingShingle | Ji Hong Kim Chae Hwan Cho Myung Yi Ryu Jong-Gil Kim Sei-Jung Lee Tae Jung Park Jong Pil Park Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1. PLoS ONE |
| title | Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1. |
| title_full | Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1. |
| title_fullStr | Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1. |
| title_full_unstemmed | Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1. |
| title_short | Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1. |
| title_sort | development of peptide biosensor for the detection of dengue fever biomarker nonstructural 1 |
| url | https://doi.org/10.1371/journal.pone.0222144 |
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