Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile
Cadmium (Cd) is an environmental pollutant, widely existing in soil, and can be absorbed and accumulated by plants. Hunan Province exhibits the worst cadmium contamination of farmland in China. Ruminants possess an abundant microbial population in the rumen, which enables them to tolerate various po...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/19/3104 |
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| author | Xinxin Xu Zebang Xu Bin Yang Kangle Yi Fang He Ao Sun Jianbo Li Yang Luo Jiakun Wang |
| author_facet | Xinxin Xu Zebang Xu Bin Yang Kangle Yi Fang He Ao Sun Jianbo Li Yang Luo Jiakun Wang |
| author_sort | Xinxin Xu |
| collection | DOAJ |
| description | Cadmium (Cd) is an environmental pollutant, widely existing in soil, and can be absorbed and accumulated by plants. Hunan Province exhibits the worst cadmium contamination of farmland in China. Ruminants possess an abundant microbial population in the rumen, which enables them to tolerate various poisonous plants. To investigate whether the rumen microbiota could respond to Cd and mitigate the toxicity of Cd-accumulated maize to ruminants, 6-month-old cattle were fed with 85.82% (fresh basis) normal whole-plant maize silage diet (CON, <i>n</i> = 10) or Cd-accumulated whole-plant maize silage diet (CAM, <i>n</i> = 10) for 107 days. When compared to the CON cattle, CAM cattle showed significantly higher gain-to-feed ratio and an increased total bacterial population in the rumen, but a decreased total bacterial population in the colon. CAM cattle had higher relative abundance of <i>Prevotella</i> and <i>Lachnospiraceae ND3007</i> group in the rumen, and <i>Lachnospiraceae NK4A136</i> group and <i>Clostridia vadinBB60</i> group in the colon. Notably, microbial correlations were enhanced in all segments of CAM cattle, especially <i>Peptostreptococcaceae</i> in the jejunum. Transcriptome analysis revealed down-regulation of several immune-related genes in the rumen of CAM cattle, and differentially expressed genes in the rumen were mostly involved in immune regulation. These findings indicated that feeding Cd-accumulated maize diet with a Cd concentration of 6.74 mg/kg dry matter (DM) could stimulate SCFA-related bacteria in the rumen, induce hormesis to promote weight gain, and improve energy utilization of cattle. |
| first_indexed | 2024-03-10T21:50:46Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-10T21:50:46Z |
| publishDate | 2023-10-01 |
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| series | Animals |
| spelling | doaj.art-9c198d6de89547a7a4515241528e617c2023-11-19T14:00:17ZengMDPI AGAnimals2076-26152023-10-011319310410.3390/ani13193104Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome ProfileXinxin Xu0Zebang Xu1Bin Yang2Kangle Yi3Fang He4Ao Sun5Jianbo Li6Yang Luo7Jiakun Wang8Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaHunan Institute of Animal and Veterinary Science, Changsha 410131, ChinaHunan Institute of Animal and Veterinary Science, Changsha 410131, ChinaHunan Institute of Animal and Veterinary Science, Changsha 410131, ChinaHunan Institute of Animal and Veterinary Science, Changsha 410131, ChinaInstitute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaCadmium (Cd) is an environmental pollutant, widely existing in soil, and can be absorbed and accumulated by plants. Hunan Province exhibits the worst cadmium contamination of farmland in China. Ruminants possess an abundant microbial population in the rumen, which enables them to tolerate various poisonous plants. To investigate whether the rumen microbiota could respond to Cd and mitigate the toxicity of Cd-accumulated maize to ruminants, 6-month-old cattle were fed with 85.82% (fresh basis) normal whole-plant maize silage diet (CON, <i>n</i> = 10) or Cd-accumulated whole-plant maize silage diet (CAM, <i>n</i> = 10) for 107 days. When compared to the CON cattle, CAM cattle showed significantly higher gain-to-feed ratio and an increased total bacterial population in the rumen, but a decreased total bacterial population in the colon. CAM cattle had higher relative abundance of <i>Prevotella</i> and <i>Lachnospiraceae ND3007</i> group in the rumen, and <i>Lachnospiraceae NK4A136</i> group and <i>Clostridia vadinBB60</i> group in the colon. Notably, microbial correlations were enhanced in all segments of CAM cattle, especially <i>Peptostreptococcaceae</i> in the jejunum. Transcriptome analysis revealed down-regulation of several immune-related genes in the rumen of CAM cattle, and differentially expressed genes in the rumen were mostly involved in immune regulation. These findings indicated that feeding Cd-accumulated maize diet with a Cd concentration of 6.74 mg/kg dry matter (DM) could stimulate SCFA-related bacteria in the rumen, induce hormesis to promote weight gain, and improve energy utilization of cattle.https://www.mdpi.com/2076-2615/13/19/3104Cdcattlerumenjejunumcolonmicrobiota |
| spellingShingle | Xinxin Xu Zebang Xu Bin Yang Kangle Yi Fang He Ao Sun Jianbo Li Yang Luo Jiakun Wang Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile Animals Cd cattle rumen jejunum colon microbiota |
| title | Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile |
| title_full | Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile |
| title_fullStr | Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile |
| title_full_unstemmed | Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile |
| title_short | Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile |
| title_sort | assessing the effects of dietary cadmium exposure on the gastrointestinal tract of beef cattle via microbiota and transcriptome profile |
| topic | Cd cattle rumen jejunum colon microbiota |
| url | https://www.mdpi.com/2076-2615/13/19/3104 |
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