Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry
Abstract Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), functions as a major structural component in crustaceans, insects and fungi and is the second most abundant polysaccharide in the nature. Although these chitin-containing organisms have been suggested as novel animal feed resources, chit...
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| Format: | Article |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-07146-3 |
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| author | Eri Tabata Akinori Kashimura Satoshi Wakita Misa Ohno Masayoshi Sakaguchi Yasusato Sugahara Yoshihiro Kino Vaclav Matoska Peter O. Bauer Fumitaka Oyama |
| author_facet | Eri Tabata Akinori Kashimura Satoshi Wakita Misa Ohno Masayoshi Sakaguchi Yasusato Sugahara Yoshihiro Kino Vaclav Matoska Peter O. Bauer Fumitaka Oyama |
| author_sort | Eri Tabata |
| collection | DOAJ |
| description | Abstract Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), functions as a major structural component in crustaceans, insects and fungi and is the second most abundant polysaccharide in the nature. Although these chitin-containing organisms have been suggested as novel animal feed resources, chitin has long been considered as indigestible fibers in the animal body. Recently, we reported that acidic chitinase (Chia) is a protease-resistant major glycosidase in mouse gastrointestinal tract (GIT) and that it digests chitin in the mouse stomach. However, the physiological role of Chia in other animals including poultry remains unknown. Here, we report that Chia can function as a digestive enzyme that breaks down chitin-containing organisms in chicken GIT. Chia mRNA is predominantly expressed in the glandular stomach tissue in normal chicken. We also show that chicken Chia has a robust chitinolytic activity at pH 2.0 and is highly resistant to proteolysis by pepsin and trypsin/chymotrypsin under conditions mimicking GIT. Chia degraded shells of mealworm larvae in the presence of digestive proteases and produced (GlcNAc)2. Thus, functional similarity of chicken Chia with the mouse enzyme suggests that chitin-containing organisms can be used for alternative poultry diets not only as whole edible resources but also as enhancers of their nutritional value. |
| first_indexed | 2024-12-19T02:45:26Z |
| format | Article |
| id | doaj.art-9c19af425e384cc88b5506bca30a9310 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T02:45:26Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9c19af425e384cc88b5506bca30a93102022-12-21T20:38:55ZengNature PortfolioScientific Reports2045-23222017-07-017111110.1038/s41598-017-07146-3Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultryEri Tabata0Akinori Kashimura1Satoshi Wakita2Misa Ohno3Masayoshi Sakaguchi4Yasusato Sugahara5Yoshihiro Kino6Vaclav Matoska7Peter O. Bauer8Fumitaka Oyama9Department of Chemistry and Life Science, Kogakuin University, HachiojiDepartment of Chemistry and Life Science, Kogakuin University, HachiojiDepartment of Chemistry and Life Science, Kogakuin University, HachiojiDepartment of Chemistry and Life Science, Kogakuin University, HachiojiDepartment of Chemistry and Life Science, Kogakuin University, HachiojiDepartment of Chemistry and Life Science, Kogakuin University, HachiojiDepartment of Bioinformatics and Molecular Neuropathology, Meiji Pharmaceutical UniversityLaboratory of Molecular Diagnostics, Department of Clinical Biochemistry, Hematology and Immunology, Homolka HospitalLaboratory of Molecular Diagnostics, Department of Clinical Biochemistry, Hematology and Immunology, Homolka HospitalDepartment of Chemistry and Life Science, Kogakuin University, HachiojiAbstract Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), functions as a major structural component in crustaceans, insects and fungi and is the second most abundant polysaccharide in the nature. Although these chitin-containing organisms have been suggested as novel animal feed resources, chitin has long been considered as indigestible fibers in the animal body. Recently, we reported that acidic chitinase (Chia) is a protease-resistant major glycosidase in mouse gastrointestinal tract (GIT) and that it digests chitin in the mouse stomach. However, the physiological role of Chia in other animals including poultry remains unknown. Here, we report that Chia can function as a digestive enzyme that breaks down chitin-containing organisms in chicken GIT. Chia mRNA is predominantly expressed in the glandular stomach tissue in normal chicken. We also show that chicken Chia has a robust chitinolytic activity at pH 2.0 and is highly resistant to proteolysis by pepsin and trypsin/chymotrypsin under conditions mimicking GIT. Chia degraded shells of mealworm larvae in the presence of digestive proteases and produced (GlcNAc)2. Thus, functional similarity of chicken Chia with the mouse enzyme suggests that chitin-containing organisms can be used for alternative poultry diets not only as whole edible resources but also as enhancers of their nutritional value.https://doi.org/10.1038/s41598-017-07146-3 |
| spellingShingle | Eri Tabata Akinori Kashimura Satoshi Wakita Misa Ohno Masayoshi Sakaguchi Yasusato Sugahara Yoshihiro Kino Vaclav Matoska Peter O. Bauer Fumitaka Oyama Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry Scientific Reports |
| title | Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry |
| title_full | Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry |
| title_fullStr | Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry |
| title_full_unstemmed | Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry |
| title_short | Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry |
| title_sort | gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin containing organisms for alternative whole edible diets for poultry |
| url | https://doi.org/10.1038/s41598-017-07146-3 |
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