Divergence in regulatory mechanisms of GR-RBP genes in different plants under abiotic stress
Abstract The IVa subfamily of glycine-rich proteins (GRPs) comprises a group of glycine-rich RNA binding proteins referred to as GR-RBPa here. Previous studies have demonstrated functions of GR-RBPa proteins in regulating stress response in plants. However, the mechanisms responsible for the differe...
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| Format: | Article |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-59341-8 |
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| author | Yingjie Zhang Yujian Mo Junyi Li Li Liu Yanhu Gao Yueqin Zhang Yongxiang Huang Lei Ren Hongbo Zhu Xingyu Jiang Yu Ling |
| author_facet | Yingjie Zhang Yujian Mo Junyi Li Li Liu Yanhu Gao Yueqin Zhang Yongxiang Huang Lei Ren Hongbo Zhu Xingyu Jiang Yu Ling |
| author_sort | Yingjie Zhang |
| collection | DOAJ |
| description | Abstract The IVa subfamily of glycine-rich proteins (GRPs) comprises a group of glycine-rich RNA binding proteins referred to as GR-RBPa here. Previous studies have demonstrated functions of GR-RBPa proteins in regulating stress response in plants. However, the mechanisms responsible for the differential regulatory functions of GR-RBPa proteins in different plant species have not been fully elucidated. In this study, we identified and comprehensively studied a total of 34 GR-RBPa proteins from five plant species. Our analysis revealed that GR-RBPa proteins were further classified into two branches, with proteins in branch I being relatively more conserved than those in branch II. When subjected to identical stresses, these genes exhibited intensive and differential expression regulation in different plant species, corresponding to the enrichment of cis-acting regulatory elements involving in environmental and internal signaling in these genes. Unexpectedly, all GR-RBPa genes in branch I underwent intensive alternative splicing (AS) regulation, while almost all genes in branch II were only constitutively spliced, despite having more introns. This study highlights the complex and divergent regulations of a group of conserved RNA binding proteins in different plants when exposed to identical stress conditions. These species-specific regulations may have implications for stress responses and adaptations in different plant species. |
| first_indexed | 2024-04-24T07:16:30Z |
| format | Article |
| id | doaj.art-9c19e52b24d9457596170c97eb2c86b1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T07:16:30Z |
| publishDate | 2024-04-01 |
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| spelling | doaj.art-9c19e52b24d9457596170c97eb2c86b12024-04-21T11:16:46ZengNature PortfolioScientific Reports2045-23222024-04-0114111710.1038/s41598-024-59341-8Divergence in regulatory mechanisms of GR-RBP genes in different plants under abiotic stressYingjie Zhang0Yujian Mo1Junyi Li2Li Liu3Yanhu Gao4Yueqin Zhang5Yongxiang Huang6Lei Ren7Hongbo Zhu8Xingyu Jiang9Yu Ling10College of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityAbstract The IVa subfamily of glycine-rich proteins (GRPs) comprises a group of glycine-rich RNA binding proteins referred to as GR-RBPa here. Previous studies have demonstrated functions of GR-RBPa proteins in regulating stress response in plants. However, the mechanisms responsible for the differential regulatory functions of GR-RBPa proteins in different plant species have not been fully elucidated. In this study, we identified and comprehensively studied a total of 34 GR-RBPa proteins from five plant species. Our analysis revealed that GR-RBPa proteins were further classified into two branches, with proteins in branch I being relatively more conserved than those in branch II. When subjected to identical stresses, these genes exhibited intensive and differential expression regulation in different plant species, corresponding to the enrichment of cis-acting regulatory elements involving in environmental and internal signaling in these genes. Unexpectedly, all GR-RBPa genes in branch I underwent intensive alternative splicing (AS) regulation, while almost all genes in branch II were only constitutively spliced, despite having more introns. This study highlights the complex and divergent regulations of a group of conserved RNA binding proteins in different plants when exposed to identical stress conditions. These species-specific regulations may have implications for stress responses and adaptations in different plant species.https://doi.org/10.1038/s41598-024-59341-8GR-RBPaAbiotic stressGene transcriptionAlternative splicingSpecies-specific |
| spellingShingle | Yingjie Zhang Yujian Mo Junyi Li Li Liu Yanhu Gao Yueqin Zhang Yongxiang Huang Lei Ren Hongbo Zhu Xingyu Jiang Yu Ling Divergence in regulatory mechanisms of GR-RBP genes in different plants under abiotic stress Scientific Reports GR-RBPa Abiotic stress Gene transcription Alternative splicing Species-specific |
| title | Divergence in regulatory mechanisms of GR-RBP genes in different plants under abiotic stress |
| title_full | Divergence in regulatory mechanisms of GR-RBP genes in different plants under abiotic stress |
| title_fullStr | Divergence in regulatory mechanisms of GR-RBP genes in different plants under abiotic stress |
| title_full_unstemmed | Divergence in regulatory mechanisms of GR-RBP genes in different plants under abiotic stress |
| title_short | Divergence in regulatory mechanisms of GR-RBP genes in different plants under abiotic stress |
| title_sort | divergence in regulatory mechanisms of gr rbp genes in different plants under abiotic stress |
| topic | GR-RBPa Abiotic stress Gene transcription Alternative splicing Species-specific |
| url | https://doi.org/10.1038/s41598-024-59341-8 |
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