Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 marker

Background: Neutrophils are the first line of defense, not only serving as he killer of microbes through phagocytosis process, in which reactive oxygen species (ROS) and anti-microbial peptides were released, but also regulating activation of immune response. CD177 is a tidylinositol glycosylphospha...

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Main Authors: Muhammad Luthfi, Tuti Kusumaningsih
Format: Article
Language:English
Published: Universitas Airlangga 2016-12-01
Series:Dental Journal
Subjects:
Online Access:http://e-journal.unair.ac.id/index.php/MKG/article/view/2753
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author Muhammad Luthfi
Tuti Kusumaningsih
author_facet Muhammad Luthfi
Tuti Kusumaningsih
author_sort Muhammad Luthfi
collection DOAJ
description Background: Neutrophils are the first line of defense, not only serving as he killer of microbes through phagocytosis process, in which reactive oxygen species (ROS) and anti-microbial peptides were released, but also regulating activation of immune response. CD177 is a tidylinositol glycosylphosphate glycoprotein with a molecular weight of 58- 64-kDa exclusively found on neutrophils, neutrophilic metamyelocytes, and mielosit. CD177 expression, a protein on the cell surface with an average size ranging from 45% to 65%, is only found on subpopulations of neutrophils. Purpose: This study aims to analyze the effects of salivary neutrophil isolation using magnetic beads and CD177 marker on S-ECC patients. Method: The study is an observational analytic research with cross sectional approach using flow cytometry analysis on the S-ECC patients and the caries-free children who were asked to use mouthwash, NaCl 1.5%. For the isolation of neutrophils, magnetic beads labeled with FITC funds and CD177+ marker were used. Result: There were 77.66% of salivary neutrophils expressing CD177+ markers, successfully isolated in the S-ECC patients, while in the caries-free children there were 63.67% of salivary neutrophils. Conclusion: In the S-ECC patients, there were 77.66% of salivary neutrophils expressing CD177markers, successfully isolated, while in the caries-free children there were 63.67% of salivary neutrophils.
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spelling doaj.art-9c1cbfe42390471b9fd0f08a25abd6842022-12-21T19:24:02ZengUniversitas AirlanggaDental Journal1978-37282442-97402016-12-01491323610.20473/j.djmkg.v49.i1.p32-362144Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 markerMuhammad Luthfi0Tuti Kusumaningsih1Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, SurabayaDepartment of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, SurabayaBackground: Neutrophils are the first line of defense, not only serving as he killer of microbes through phagocytosis process, in which reactive oxygen species (ROS) and anti-microbial peptides were released, but also regulating activation of immune response. CD177 is a tidylinositol glycosylphosphate glycoprotein with a molecular weight of 58- 64-kDa exclusively found on neutrophils, neutrophilic metamyelocytes, and mielosit. CD177 expression, a protein on the cell surface with an average size ranging from 45% to 65%, is only found on subpopulations of neutrophils. Purpose: This study aims to analyze the effects of salivary neutrophil isolation using magnetic beads and CD177 marker on S-ECC patients. Method: The study is an observational analytic research with cross sectional approach using flow cytometry analysis on the S-ECC patients and the caries-free children who were asked to use mouthwash, NaCl 1.5%. For the isolation of neutrophils, magnetic beads labeled with FITC funds and CD177+ marker were used. Result: There were 77.66% of salivary neutrophils expressing CD177+ markers, successfully isolated in the S-ECC patients, while in the caries-free children there were 63.67% of salivary neutrophils. Conclusion: In the S-ECC patients, there were 77.66% of salivary neutrophils expressing CD177markers, successfully isolated, while in the caries-free children there were 63.67% of salivary neutrophils.http://e-journal.unair.ac.id/index.php/MKG/article/view/2753magnetic beadssalivary neutrophilsS-ECC
spellingShingle Muhammad Luthfi
Tuti Kusumaningsih
Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 marker
Dental Journal
magnetic beads
salivary neutrophils
S-ECC
title Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 marker
title_full Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 marker
title_fullStr Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 marker
title_full_unstemmed Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 marker
title_short Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 marker
title_sort salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and cd177 marker
topic magnetic beads
salivary neutrophils
S-ECC
url http://e-journal.unair.ac.id/index.php/MKG/article/view/2753
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AT tutikusumaningsih salivaryneutrophilsisolationofsevereearlychildhoodcariespatientswithflowcytometryanalysisusingmagneticbeadsandcd177marker