| Summary: | One of the most prevalent causes of foodborne illnesses worldwide is staphylococcal food poisoning. This study aimed to provide a robust method to extract the bacteria <i>Staphylococcus aureus</i> from food samples using glycan-coated magnetic nanoparticles (MNPs). Then, a cost-effective multi-probe genomic biosensor was designed to detect the nuc gene of <i>S. aureus</i> rapidly in different food matrices. This biosensor utilized gold nanoparticles and two DNA oligonucleotide probes combined to produce a plasmonic/colorimetric response to inform users if the sample was positive for <i>S. aureus</i>. In addition, the specificity and sensitivity of the biosensor were determined. For the specificity trials, the <i>S. aureus</i> biosensor was compared with the extracted DNA of <i>Escherichia coli</i>, <i>Salmonella enterica</i> serovar Enteritidis (SE), and <i>Bacillus cereus.</i> The sensitivity tests showed that the biosensor could detect as low as 2.5 ng/µL of the target DNA with a linear range of up to 20 ng/µL of DNA. With further research, this simple and cost-effective biosensor can rapidly identify foodborne pathogens from large-volume samples.
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