Effects of exogenous advanced glycation end products on oxidative stress and renal injury in healthy mice
Abstract This study evaluated the biological effects of exogenous advanced glycation end products (AGEs) on the induction of chronic kidney disease and the dose‐effect relationship. Male C57BL/6 mice were placed on four diets, including saline and three other diets differing only in AGEs content (lo...
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| Format: | Article |
| Language: | English |
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Wiley
2023-08-01
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| Series: | eFood |
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| Online Access: | https://doi.org/10.1002/efd2.105 |
| _version_ | 1797743800813092864 |
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| author | Lan Zhang Pingping Wen Jixin Zhang Chao Xia Jingguo Xu Huiqing Xu Guiyou Cui Jun Wang |
| author_facet | Lan Zhang Pingping Wen Jixin Zhang Chao Xia Jingguo Xu Huiqing Xu Guiyou Cui Jun Wang |
| author_sort | Lan Zhang |
| collection | DOAJ |
| description | Abstract This study evaluated the biological effects of exogenous advanced glycation end products (AGEs) on the induction of chronic kidney disease and the dose‐effect relationship. Male C57BL/6 mice were placed on four diets, including saline and three other diets differing only in AGEs content (low‐AGEs [LA], medium‐AGEs [MA], and high‐AGEs [HA] ratio, 1:3:5) for 4 weeks. With the increasing intake of AGEs, mice developed a significant increase in blood glucose and lipid levels, the fluorescence intensity of AGEs, Nε‐(carboxymethyl)‐lysine, Nε‐(carboxyethyl)‐lysine, and malondialdehyde levels, whereas their superoxide dismutase activity and glutathione levels were decreased significantly. HA had the highest urinary protein levels and the lowest creatinine clearance compared to the other groups. These suggested that AGEs are an essential contributor to increasing oxidative stress levels and intake of high‐level AGEs induces more severe kidney function impairment. Meanwhile, the AGEs intake damaged the kidney structure in a dose‐dependent manner, as evidenced by granular degeneration of kidney tubular epithelial cells and inflammatory cell infiltration. These findings shed light on the detrimental impacts of AGEs on human kidneys, which also will help reveal a dose‐effect relationship of AGEs. |
| first_indexed | 2024-03-12T15:00:40Z |
| format | Article |
| id | doaj.art-9c217537dbb34338a7d9bd0867ba32f0 |
| institution | Directory Open Access Journal |
| issn | 2666-3066 |
| language | English |
| last_indexed | 2024-03-12T15:00:40Z |
| publishDate | 2023-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | eFood |
| spelling | doaj.art-9c217537dbb34338a7d9bd0867ba32f02023-08-14T08:22:27ZengWileyeFood2666-30662023-08-0144n/an/a10.1002/efd2.105Effects of exogenous advanced glycation end products on oxidative stress and renal injury in healthy miceLan Zhang0Pingping Wen1Jixin Zhang2Chao Xia3Jingguo Xu4Huiqing Xu5Guiyou Cui6Jun Wang7Tourism and Cuisine College Yangzhou University Yangzhou ChinaTourism and Cuisine College Yangzhou University Yangzhou ChinaTourism and Cuisine College Yangzhou University Yangzhou ChinaTourism and Cuisine College Yangzhou University Yangzhou ChinaTourism and Cuisine College Yangzhou University Yangzhou ChinaTourism and Cuisine College Yangzhou University Yangzhou ChinaTourism and Cuisine College Yangzhou University Yangzhou ChinaTourism and Cuisine College Yangzhou University Yangzhou ChinaAbstract This study evaluated the biological effects of exogenous advanced glycation end products (AGEs) on the induction of chronic kidney disease and the dose‐effect relationship. Male C57BL/6 mice were placed on four diets, including saline and three other diets differing only in AGEs content (low‐AGEs [LA], medium‐AGEs [MA], and high‐AGEs [HA] ratio, 1:3:5) for 4 weeks. With the increasing intake of AGEs, mice developed a significant increase in blood glucose and lipid levels, the fluorescence intensity of AGEs, Nε‐(carboxymethyl)‐lysine, Nε‐(carboxyethyl)‐lysine, and malondialdehyde levels, whereas their superoxide dismutase activity and glutathione levels were decreased significantly. HA had the highest urinary protein levels and the lowest creatinine clearance compared to the other groups. These suggested that AGEs are an essential contributor to increasing oxidative stress levels and intake of high‐level AGEs induces more severe kidney function impairment. Meanwhile, the AGEs intake damaged the kidney structure in a dose‐dependent manner, as evidenced by granular degeneration of kidney tubular epithelial cells and inflammatory cell infiltration. These findings shed light on the detrimental impacts of AGEs on human kidneys, which also will help reveal a dose‐effect relationship of AGEs.https://doi.org/10.1002/efd2.105dose‐effect relationshipexogenous advanced glycation end productskidney functionkidney tubulesoxidative stress |
| spellingShingle | Lan Zhang Pingping Wen Jixin Zhang Chao Xia Jingguo Xu Huiqing Xu Guiyou Cui Jun Wang Effects of exogenous advanced glycation end products on oxidative stress and renal injury in healthy mice eFood dose‐effect relationship exogenous advanced glycation end products kidney function kidney tubules oxidative stress |
| title | Effects of exogenous advanced glycation end products on oxidative stress and renal injury in healthy mice |
| title_full | Effects of exogenous advanced glycation end products on oxidative stress and renal injury in healthy mice |
| title_fullStr | Effects of exogenous advanced glycation end products on oxidative stress and renal injury in healthy mice |
| title_full_unstemmed | Effects of exogenous advanced glycation end products on oxidative stress and renal injury in healthy mice |
| title_short | Effects of exogenous advanced glycation end products on oxidative stress and renal injury in healthy mice |
| title_sort | effects of exogenous advanced glycation end products on oxidative stress and renal injury in healthy mice |
| topic | dose‐effect relationship exogenous advanced glycation end products kidney function kidney tubules oxidative stress |
| url | https://doi.org/10.1002/efd2.105 |
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