A comparative study of ethylene emanation upon nitrogen deficiency in natural accessions of Arabidopsis thaliana.
An original approach to develop sustainable agriculture with less nitrogen fertilizer inputs is to tackle the cross-talk between nitrogen nutrition and plant growth regulators. In particular the gaseous hormone, ethylene, is a prime target for that purpose. The variation of ethylene production in na...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-02-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00070/full |
| _version_ | 1818424863310741504 |
|---|---|
| author | Hugues eDe Gernier Jérôme eDe Pessemier Jiajia eXu Simona M Cristescu Dominique eVan Der Straeten Nathalie eVerbruggen Christian eHermans |
| author_facet | Hugues eDe Gernier Jérôme eDe Pessemier Jiajia eXu Simona M Cristescu Dominique eVan Der Straeten Nathalie eVerbruggen Christian eHermans |
| author_sort | Hugues eDe Gernier |
| collection | DOAJ |
| description | An original approach to develop sustainable agriculture with less nitrogen fertilizer inputs is to tackle the cross-talk between nitrogen nutrition and plant growth regulators. In particular the gaseous hormone, ethylene, is a prime target for that purpose. The variation of ethylene production in natural accessions of the model species Arabidopsis thaliana was explored in response to the nitrate supply. Ethylene was measured with a laser-based photoacoustic detector. First, experimental conditions were established with Columbia-0 (Col-0) accession, which was grown in vitro on horizontal plates across a range of five nitrate concentrations (0.5, 1, 2.5, 5 and 10 mM). The concentrations of 1 and 10 mM nitrate were retained for further characterization. Along with a decrease of total dry biomass and higher biomass allocation to the roots, the ethylene production was 50% more important at 1 mM than at 10 mM nitrate. The total transcript levels of 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASES (ACS) in roots and those of ACC OXIDASES (ACO) in shoots increased by 100% between the same treatments. This was mainly due to higher transcript levels of ACS6 and of ACO2 and ACO4. The assumption was that during nitrogen deficiency, the greater biomass allocation in favor of the roots was controlled by ethylene being released in the shoots after conversion of ACC originating from the roots. Second, biomass and ethylene productions were measured in twenty accessions. Across all accessions, the total dry biomass and ethylene production were correlated negatively at 1 mM but positively at 10 mM nitrate. Furthermore, polymorphism was surveyed in ACC and ethylene biosynthesis genes and gene products among accessions. Very few substitutions modifying the amino acids properties in conserved motifs of the enzymes were found in the accessions. Natural variation of ethylene production could be further explored to improve Nitrogen Use Efficiency, in particular by manipulating features like the biomass production and the timing of senescence upon nitrogen limitation. |
| first_indexed | 2024-12-14T14:04:48Z |
| format | Article |
| id | doaj.art-9c23a1f8317949418a8229c338a52c7b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T14:04:48Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9c23a1f8317949418a8229c338a52c7b2022-12-21T22:58:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-02-01710.3389/fpls.2016.00070170696A comparative study of ethylene emanation upon nitrogen deficiency in natural accessions of Arabidopsis thaliana.Hugues eDe Gernier0Jérôme eDe Pessemier1Jiajia eXu2Simona M Cristescu3Dominique eVan Der Straeten4Nathalie eVerbruggen5Christian eHermans6Université Libre de BruxellesUniversité Libre de BruxellesUniversité Libre de BruxellesRadboud UniversityGhent UniversityUniversité Libre de BruxellesUniversité Libre de BruxellesAn original approach to develop sustainable agriculture with less nitrogen fertilizer inputs is to tackle the cross-talk between nitrogen nutrition and plant growth regulators. In particular the gaseous hormone, ethylene, is a prime target for that purpose. The variation of ethylene production in natural accessions of the model species Arabidopsis thaliana was explored in response to the nitrate supply. Ethylene was measured with a laser-based photoacoustic detector. First, experimental conditions were established with Columbia-0 (Col-0) accession, which was grown in vitro on horizontal plates across a range of five nitrate concentrations (0.5, 1, 2.5, 5 and 10 mM). The concentrations of 1 and 10 mM nitrate were retained for further characterization. Along with a decrease of total dry biomass and higher biomass allocation to the roots, the ethylene production was 50% more important at 1 mM than at 10 mM nitrate. The total transcript levels of 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASES (ACS) in roots and those of ACC OXIDASES (ACO) in shoots increased by 100% between the same treatments. This was mainly due to higher transcript levels of ACS6 and of ACO2 and ACO4. The assumption was that during nitrogen deficiency, the greater biomass allocation in favor of the roots was controlled by ethylene being released in the shoots after conversion of ACC originating from the roots. Second, biomass and ethylene productions were measured in twenty accessions. Across all accessions, the total dry biomass and ethylene production were correlated negatively at 1 mM but positively at 10 mM nitrate. Furthermore, polymorphism was surveyed in ACC and ethylene biosynthesis genes and gene products among accessions. Very few substitutions modifying the amino acids properties in conserved motifs of the enzymes were found in the accessions. Natural variation of ethylene production could be further explored to improve Nitrogen Use Efficiency, in particular by manipulating features like the biomass production and the timing of senescence upon nitrogen limitation.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00070/fullArabidopsis thaliananatural variationbiomass productionNitrogen nutritionEthylene production |
| spellingShingle | Hugues eDe Gernier Jérôme eDe Pessemier Jiajia eXu Simona M Cristescu Dominique eVan Der Straeten Nathalie eVerbruggen Christian eHermans A comparative study of ethylene emanation upon nitrogen deficiency in natural accessions of Arabidopsis thaliana. Frontiers in Plant Science Arabidopsis thaliana natural variation biomass production Nitrogen nutrition Ethylene production |
| title | A comparative study of ethylene emanation upon nitrogen deficiency in natural accessions of Arabidopsis thaliana. |
| title_full | A comparative study of ethylene emanation upon nitrogen deficiency in natural accessions of Arabidopsis thaliana. |
| title_fullStr | A comparative study of ethylene emanation upon nitrogen deficiency in natural accessions of Arabidopsis thaliana. |
| title_full_unstemmed | A comparative study of ethylene emanation upon nitrogen deficiency in natural accessions of Arabidopsis thaliana. |
| title_short | A comparative study of ethylene emanation upon nitrogen deficiency in natural accessions of Arabidopsis thaliana. |
| title_sort | comparative study of ethylene emanation upon nitrogen deficiency in natural accessions of arabidopsis thaliana |
| topic | Arabidopsis thaliana natural variation biomass production Nitrogen nutrition Ethylene production |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00070/full |
| work_keys_str_mv | AT huguesedegernier acomparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT jeromeedepessemier acomparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT jiajiaexu acomparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT simonamcristescu acomparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT dominiqueevanderstraeten acomparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT nathalieeverbruggen acomparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT christianehermans acomparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT huguesedegernier comparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT jeromeedepessemier comparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT jiajiaexu comparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT simonamcristescu comparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT dominiqueevanderstraeten comparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT nathalieeverbruggen comparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana AT christianehermans comparativestudyofethyleneemanationuponnitrogendeficiencyinnaturalaccessionsofarabidopsisthaliana |