A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation
MoS2 nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS2 nanosheets and restriction endonuclease BstUI for methylation analysis of p16 pr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-09-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/16/10/1561 |
| _version_ | 1798040534972891136 |
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| author | Le Xiao Li Xu Chuan Gao Yulin Zhang Qunfeng Yao Guo-Jun Zhang |
| author_facet | Le Xiao Li Xu Chuan Gao Yulin Zhang Qunfeng Yao Guo-Jun Zhang |
| author_sort | Le Xiao |
| collection | DOAJ |
| description | MoS2 nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS2 nanosheets and restriction endonuclease BstUI for methylation analysis of p16 promoter. The biosensing platform exhibited excellent sensitivity in detecting DNA with a linear range of 100 pM~20 nM and a detection limit of 140 pM. More importantly, our method could distinguish as low as 1% difference in methylation level. Compared with previous methylation analysis, our design is both time saving and simple to operate, avoiding the limitations of PCR-based assays without compromising performance. |
| first_indexed | 2024-04-11T22:08:58Z |
| format | Article |
| id | doaj.art-9c2bbd3835204b10a4fef602095b9feb |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:08:58Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-9c2bbd3835204b10a4fef602095b9feb2022-12-22T04:00:36ZengMDPI AGSensors1424-82202016-09-011610156110.3390/s16101561s16101561A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA MethylationLe Xiao0Li Xu1Chuan Gao2Yulin Zhang3Qunfeng Yao4Guo-Jun Zhang5School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Pharmacy, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, ChinaMoS2 nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS2 nanosheets and restriction endonuclease BstUI for methylation analysis of p16 promoter. The biosensing platform exhibited excellent sensitivity in detecting DNA with a linear range of 100 pM~20 nM and a detection limit of 140 pM. More importantly, our method could distinguish as low as 1% difference in methylation level. Compared with previous methylation analysis, our design is both time saving and simple to operate, avoiding the limitations of PCR-based assays without compromising performance.http://www.mdpi.com/1424-8220/16/10/1561MoS2fluorescence biosensorhomogeneous analysisDNA methylation |
| spellingShingle | Le Xiao Li Xu Chuan Gao Yulin Zhang Qunfeng Yao Guo-Jun Zhang A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation Sensors MoS2 fluorescence biosensor homogeneous analysis DNA methylation |
| title | A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation |
| title_full | A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation |
| title_fullStr | A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation |
| title_full_unstemmed | A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation |
| title_short | A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation |
| title_sort | mos2 nanosheet based fluorescence biosensor for simple and quantitative analysis of dna methylation |
| topic | MoS2 fluorescence biosensor homogeneous analysis DNA methylation |
| url | http://www.mdpi.com/1424-8220/16/10/1561 |
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