Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test
Background: Human echinococcosis is a neglected zoonotic disease distributed worldwide. It comprises cystic and alveolar forms, the former being the more prevalent disease. Imaging techniques are the first choice for diagnosis of cystic echinococcosis and serology is used as an additional diagnostic...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-09-01
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| Series: | Iranian Journal of Parasitology |
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| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3035 |
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| author | Rahmah NOORDIN Sam KHANBABAIE Muhammad HAFIZNUR YUNUS Hanspeter MARTI Beatrice NICKEL Majid FASIHI HARANDI Saeid NASIBI |
| author_facet | Rahmah NOORDIN Sam KHANBABAIE Muhammad HAFIZNUR YUNUS Hanspeter MARTI Beatrice NICKEL Majid FASIHI HARANDI Saeid NASIBI |
| author_sort | Rahmah NOORDIN |
| collection | DOAJ |
| description | Background: Human echinococcosis is a neglected zoonotic disease distributed worldwide. It comprises cystic and alveolar forms, the former being the more prevalent disease. Imaging techniques are the first choice for diagnosis of cystic echinococcosis and serology is used as an additional diagnostic technique in doubtful cases or as the sole test in low-resource settings. Rapid diagnostic tests are useful and convenient for immunodiagnosis of cystic echinococcosis in endemic areas, where medical facilities often struggle with limited resources.
Methods: Recently, we have developed Hyd Rapid™, an IgG4 lateral flow dipstick test using recombinant antigen B1 for detection of cystic echinococcosis. This study was performed between 2016 until 2018 at the Institute for Research in Molecular Medicine, Universiti Sains Malaysia. The diagnostic performance of Hyd Rapid™ was tested in-house and at two international laboratories in Switzerland and Iran.
Results: The overall diagnostic sensitivity for detection of cystic and alveolar echinococcosis was 95% (56/59). Meanwhile, the diagnostic specificity, with and without exclusion of cysticercosis and fascioliasis, was 100% (n=48) and 88% (63/72), respectively.
Conclusion: Hyd Rapid™ detected cystic echinococcosis as well as probable cases of alveolar echinococcosis. Therefore, Hyd Rapid™ showed good potential as a serological tool for echinococcosis, and merits further evaluation. |
| first_indexed | 2024-12-16T11:19:31Z |
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| id | doaj.art-9c2c495134c049e5a5d1c963cd99eaf9 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-16T11:19:31Z |
| publishDate | 2020-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-9c2c495134c049e5a5d1c963cd99eaf92022-12-21T22:33:30ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2020-09-0115329029810.18502/ijpa.v15i3.41913035Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick TestRahmah NOORDIN0Sam KHANBABAIE1Muhammad HAFIZNUR YUNUS2Hanspeter MARTI3Beatrice NICKEL4Majid FASIHI HARANDI5Saeid NASIBI61. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia1. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia1. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Pulau Pinang, MalaysiaSwiss Tropical and Public Health Institute, Basel, Switzerland .University of Basel, Basel, SwitzerlandSwiss Tropical and Public Health Institute, Basel, Switzerland .University of Basel, Basel, SwitzerlandResearch Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, IranResearch Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, IranBackground: Human echinococcosis is a neglected zoonotic disease distributed worldwide. It comprises cystic and alveolar forms, the former being the more prevalent disease. Imaging techniques are the first choice for diagnosis of cystic echinococcosis and serology is used as an additional diagnostic technique in doubtful cases or as the sole test in low-resource settings. Rapid diagnostic tests are useful and convenient for immunodiagnosis of cystic echinococcosis in endemic areas, where medical facilities often struggle with limited resources. Methods: Recently, we have developed Hyd Rapid™, an IgG4 lateral flow dipstick test using recombinant antigen B1 for detection of cystic echinococcosis. This study was performed between 2016 until 2018 at the Institute for Research in Molecular Medicine, Universiti Sains Malaysia. The diagnostic performance of Hyd Rapid™ was tested in-house and at two international laboratories in Switzerland and Iran. Results: The overall diagnostic sensitivity for detection of cystic and alveolar echinococcosis was 95% (56/59). Meanwhile, the diagnostic specificity, with and without exclusion of cysticercosis and fascioliasis, was 100% (n=48) and 88% (63/72), respectively. Conclusion: Hyd Rapid™ detected cystic echinococcosis as well as probable cases of alveolar echinococcosis. Therefore, Hyd Rapid™ showed good potential as a serological tool for echinococcosis, and merits further evaluation.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3035rapid testlateral flowcystic and alveolar echinococcosisevaluationimmunoglobulin g |
| spellingShingle | Rahmah NOORDIN Sam KHANBABAIE Muhammad HAFIZNUR YUNUS Hanspeter MARTI Beatrice NICKEL Majid FASIHI HARANDI Saeid NASIBI Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test Iranian Journal of Parasitology rapid test lateral flow cystic and alveolar echinococcosis evaluation immunoglobulin g |
| title | Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test |
| title_full | Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test |
| title_fullStr | Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test |
| title_full_unstemmed | Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test |
| title_short | Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test |
| title_sort | evaluation of the diagnostic performance of recombinant antigen b1 for detection of cystic echinococcosis using lateral flow dipstick test |
| topic | rapid test lateral flow cystic and alveolar echinococcosis evaluation immunoglobulin g |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3035 |
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