An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil

Faeces from 17 children less than 1.6 years old 15 adultsmore than 22 years old were collected during an outbreak of gastroenteritis in aday care nursery and screened for the presence of adenovirus and rotavirus by enzyme immunoassay (EIARA) and other viruses by electron microscopy (EM) and polycryl...

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Main Authors: Liane de Castro, Dalia dos Prazeres Rodrigues, Regina Flauzino, Marta Moura, José Paulo Gagliardi Leite
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 1994-03-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000100002
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author Liane de Castro
Dalia dos Prazeres Rodrigues
Regina Flauzino
Marta Moura
José Paulo Gagliardi Leite
author_facet Liane de Castro
Dalia dos Prazeres Rodrigues
Regina Flauzino
Marta Moura
José Paulo Gagliardi Leite
author_sort Liane de Castro
collection DOAJ
description Faeces from 17 children less than 1.6 years old 15 adultsmore than 22 years old were collected during an outbreak of gastroenteritis in aday care nursery and screened for the presence of adenovirus and rotavirus by enzyme immunoassay (EIARA) and other viruses by electron microscopy (EM) and polycrylamide gel electrophoresis (PAGE). Ten samples (58.8 per cent) from childrenand one (6.7 per cent) from adults were positive for rotavirus and all samples were negative for bacteria and parasites. No other viruses were observed in EM. An enzyme immunoassay test using monoclonal antibodies (MAb-EIA) to determine the subgroup(s) and the serotype(s) of rotavirus was performed and the results showedthat all positive samples belong to serotype 1, subgroup II of group A rotaviruses. In PAGE test all samples had the same profile and the 10 and 11 dsRNA segments corresponed to the "long" profile of group A of rotaviruses. These results corroborated the MAbEIA results and indicate a sole source of infection. The majorsymptoms observed were: vomiting (60 per cent), fever (70 per cent) and diarrhoea (100 per cent). In previous years (1989 to 1991) we observed only rotavirus serotype 2 in this same day care nursery, but no outbreak was reported.
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spelling doaj.art-9c2deb4231fb44bca9b66dcfc535a4fe2023-08-02T09:28:22ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601994-03-018915910.1590/S0074-02761994000100002An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, BrazilLiane de CastroDalia dos Prazeres RodriguesRegina FlauzinoMarta MouraJosé Paulo Gagliardi LeiteFaeces from 17 children less than 1.6 years old 15 adultsmore than 22 years old were collected during an outbreak of gastroenteritis in aday care nursery and screened for the presence of adenovirus and rotavirus by enzyme immunoassay (EIARA) and other viruses by electron microscopy (EM) and polycrylamide gel electrophoresis (PAGE). Ten samples (58.8 per cent) from childrenand one (6.7 per cent) from adults were positive for rotavirus and all samples were negative for bacteria and parasites. No other viruses were observed in EM. An enzyme immunoassay test using monoclonal antibodies (MAb-EIA) to determine the subgroup(s) and the serotype(s) of rotavirus was performed and the results showedthat all positive samples belong to serotype 1, subgroup II of group A rotaviruses. In PAGE test all samples had the same profile and the 10 and 11 dsRNA segments corresponed to the "long" profile of group A of rotaviruses. These results corroborated the MAbEIA results and indicate a sole source of infection. The majorsymptoms observed were: vomiting (60 per cent), fever (70 per cent) and diarrhoea (100 per cent). In previous years (1989 to 1991) we observed only rotavirus serotype 2 in this same day care nursery, but no outbreak was reported.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000100002diarrhoeaoutbreakrotavirusserotype 1
spellingShingle Liane de Castro
Dalia dos Prazeres Rodrigues
Regina Flauzino
Marta Moura
José Paulo Gagliardi Leite
An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil
Memorias do Instituto Oswaldo Cruz
diarrhoea
outbreak
rotavirus
serotype 1
title An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil
title_full An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil
title_fullStr An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil
title_full_unstemmed An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil
title_short An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil
title_sort outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in rio de janeiro brazil
topic diarrhoea
outbreak
rotavirus
serotype 1
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000100002
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