An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil
Faeces from 17 children less than 1.6 years old 15 adultsmore than 22 years old were collected during an outbreak of gastroenteritis in aday care nursery and screened for the presence of adenovirus and rotavirus by enzyme immunoassay (EIARA) and other viruses by electron microscopy (EM) and polycryl...
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Format: | Article |
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Fundação Oswaldo Cruz (FIOCRUZ)
1994-03-01
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Series: | Memorias do Instituto Oswaldo Cruz |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000100002 |
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author | Liane de Castro Dalia dos Prazeres Rodrigues Regina Flauzino Marta Moura José Paulo Gagliardi Leite |
author_facet | Liane de Castro Dalia dos Prazeres Rodrigues Regina Flauzino Marta Moura José Paulo Gagliardi Leite |
author_sort | Liane de Castro |
collection | DOAJ |
description | Faeces from 17 children less than 1.6 years old 15 adultsmore than 22 years old were collected during an outbreak of gastroenteritis in aday care nursery and screened for the presence of adenovirus and rotavirus by enzyme immunoassay (EIARA) and other viruses by electron microscopy (EM) and polycrylamide gel electrophoresis (PAGE). Ten samples (58.8 per cent) from childrenand one (6.7 per cent) from adults were positive for rotavirus and all samples were negative for bacteria and parasites. No other viruses were observed in EM. An enzyme immunoassay test using monoclonal antibodies (MAb-EIA) to determine the subgroup(s) and the serotype(s) of rotavirus was performed and the results showedthat all positive samples belong to serotype 1, subgroup II of group A rotaviruses. In PAGE test all samples had the same profile and the 10 and 11 dsRNA segments corresponed to the "long" profile of group A of rotaviruses. These results corroborated the MAbEIA results and indicate a sole source of infection. The majorsymptoms observed were: vomiting (60 per cent), fever (70 per cent) and diarrhoea (100 per cent). In previous years (1989 to 1991) we observed only rotavirus serotype 2 in this same day care nursery, but no outbreak was reported. |
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institution | Directory Open Access Journal |
issn | 0074-0276 1678-8060 |
language | English |
last_indexed | 2024-03-12T18:06:35Z |
publishDate | 1994-03-01 |
publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
record_format | Article |
series | Memorias do Instituto Oswaldo Cruz |
spelling | doaj.art-9c2deb4231fb44bca9b66dcfc535a4fe2023-08-02T09:28:22ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601994-03-018915910.1590/S0074-02761994000100002An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, BrazilLiane de CastroDalia dos Prazeres RodriguesRegina FlauzinoMarta MouraJosé Paulo Gagliardi LeiteFaeces from 17 children less than 1.6 years old 15 adultsmore than 22 years old were collected during an outbreak of gastroenteritis in aday care nursery and screened for the presence of adenovirus and rotavirus by enzyme immunoassay (EIARA) and other viruses by electron microscopy (EM) and polycrylamide gel electrophoresis (PAGE). Ten samples (58.8 per cent) from childrenand one (6.7 per cent) from adults were positive for rotavirus and all samples were negative for bacteria and parasites. No other viruses were observed in EM. An enzyme immunoassay test using monoclonal antibodies (MAb-EIA) to determine the subgroup(s) and the serotype(s) of rotavirus was performed and the results showedthat all positive samples belong to serotype 1, subgroup II of group A rotaviruses. In PAGE test all samples had the same profile and the 10 and 11 dsRNA segments corresponed to the "long" profile of group A of rotaviruses. These results corroborated the MAbEIA results and indicate a sole source of infection. The majorsymptoms observed were: vomiting (60 per cent), fever (70 per cent) and diarrhoea (100 per cent). In previous years (1989 to 1991) we observed only rotavirus serotype 2 in this same day care nursery, but no outbreak was reported.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000100002diarrhoeaoutbreakrotavirusserotype 1 |
spellingShingle | Liane de Castro Dalia dos Prazeres Rodrigues Regina Flauzino Marta Moura José Paulo Gagliardi Leite An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil Memorias do Instituto Oswaldo Cruz diarrhoea outbreak rotavirus serotype 1 |
title | An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil |
title_full | An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil |
title_fullStr | An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil |
title_full_unstemmed | An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil |
title_short | An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil |
title_sort | outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in rio de janeiro brazil |
topic | diarrhoea outbreak rotavirus serotype 1 |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000100002 |
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