Chemical constituents, antioxidant and antimicrobial activities of Pterygota alata (Roxb.) leaves extracts grown in Egypt
Overproduction of reactive species and microbial resistance to the existent antibiotics are still great health challenges. The current study aimed to evaluate the antioxidant and antimicrobial activities of Pterygota alata leaves extracts, in addition to isolation and identification of their chemi...
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| Format: | Article |
| Language: | English |
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The National Information and Documentation Centre (NIDOC) , Egypt
2019-06-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_37210_9ab2a6b8dcefd58767104e201c74c776.pdf |
| _version_ | 1797203517380755456 |
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| author | Maha E. Omran Azza A. Shafei Sally E. Abdel-Rahman |
| author_facet | Maha E. Omran Azza A. Shafei Sally E. Abdel-Rahman |
| author_sort | Maha E. Omran |
| collection | DOAJ |
| description | Overproduction of reactive species and microbial resistance to the existent antibiotics are still great health
challenges. The current study aimed to evaluate the antioxidant and antimicrobial activities of Pterygota alata
leaves extracts, in addition to isolation and identification of their chemical constituents. In vitro antioxidant
activity was explored using 2,2'-diphenyl-1-picrylhydrazyl radical scavenging assay (DPPH), whereas the in vitro
antimicrobial activity was evaluated using the disc agar plate method. Chemical constituents of the leaves extracts
were isolated through column chromatography, and then identified using 1H, 13C-NMR and IR spectroscopic
tools. In the DPPH assay; the half maximal inhibitory concentration (IC50) value of the total extract was 72.4 ± 1.4
µg/ ml, relative to 11.2 ± 0.6 µg/ ml of the standard ascorbic acid. On the other hand; the antimicrobial results
revealed that the ethyl acetate extract showed the strongest inhibitory potential against most of the tested microbes
with inhibition zones of 16.8- 22.8 mm, followed by petroleum ether with inhibition zones between 13.4- 19.6
mm, and n-BuOH with inhibition zones ranging from 14.9-19.3 mm. These results were compared with the
standard antibiotics such as; Amphatricin β, Ampicillin, and Gentamycin. Furthermore; chromatographic isolation
of the different solvents extracts resulted in the isolation of five phytoconstituents, their chemical structures were
assigned as; β-sitosterol (1), apigenin-7-β-D-glycoside (2), gallic acid (3), luteolin-7-β-D-glucoside (4), and 4'-
methoxy myricetin-3-β-D-glucoside (5). Current findings suggested that leaf extracts of P. alata could be used for
the development of natural drugs; to treat microbial infections and reactive oxygen species (ROS) associated
disorders. |
| first_indexed | 2024-04-24T08:20:35Z |
| format | Article |
| id | doaj.art-9c2df9d2b50541a7af8eb58f4da0cff0 |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:20:35Z |
| publishDate | 2019-06-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-9c2df9d2b50541a7af8eb58f4da0cff02024-04-17T01:38:58ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942019-06-013336637810.21608/nrmj.2019.37210Chemical constituents, antioxidant and antimicrobial activities of Pterygota alata (Roxb.) leaves extracts grown in EgyptMaha E. Omran0 Azza A. Shafei1Sally E. Abdel-Rahman2Microbiology Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, EgyptPharmacognosy Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, EgyptPharmacognosy Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, EgyptOverproduction of reactive species and microbial resistance to the existent antibiotics are still great health challenges. The current study aimed to evaluate the antioxidant and antimicrobial activities of Pterygota alata leaves extracts, in addition to isolation and identification of their chemical constituents. In vitro antioxidant activity was explored using 2,2'-diphenyl-1-picrylhydrazyl radical scavenging assay (DPPH), whereas the in vitro antimicrobial activity was evaluated using the disc agar plate method. Chemical constituents of the leaves extracts were isolated through column chromatography, and then identified using 1H, 13C-NMR and IR spectroscopic tools. In the DPPH assay; the half maximal inhibitory concentration (IC50) value of the total extract was 72.4 ± 1.4 µg/ ml, relative to 11.2 ± 0.6 µg/ ml of the standard ascorbic acid. On the other hand; the antimicrobial results revealed that the ethyl acetate extract showed the strongest inhibitory potential against most of the tested microbes with inhibition zones of 16.8- 22.8 mm, followed by petroleum ether with inhibition zones between 13.4- 19.6 mm, and n-BuOH with inhibition zones ranging from 14.9-19.3 mm. These results were compared with the standard antibiotics such as; Amphatricin β, Ampicillin, and Gentamycin. Furthermore; chromatographic isolation of the different solvents extracts resulted in the isolation of five phytoconstituents, their chemical structures were assigned as; β-sitosterol (1), apigenin-7-β-D-glycoside (2), gallic acid (3), luteolin-7-β-D-glucoside (4), and 4'- methoxy myricetin-3-β-D-glucoside (5). Current findings suggested that leaf extracts of P. alata could be used for the development of natural drugs; to treat microbial infections and reactive oxygen species (ROS) associated disorders.https://nrmj.journals.ekb.eg/article_37210_9ab2a6b8dcefd58767104e201c74c776.pdfpterygota alata (roxb.)phenolicsantioxidantantimicrobial activities |
| spellingShingle | Maha E. Omran Azza A. Shafei Sally E. Abdel-Rahman Chemical constituents, antioxidant and antimicrobial activities of Pterygota alata (Roxb.) leaves extracts grown in Egypt Novel Research in Microbiology Journal pterygota alata (roxb.) phenolics antioxidant antimicrobial activities |
| title | Chemical constituents, antioxidant and antimicrobial activities of Pterygota alata (Roxb.) leaves extracts grown in Egypt |
| title_full | Chemical constituents, antioxidant and antimicrobial activities of Pterygota alata (Roxb.) leaves extracts grown in Egypt |
| title_fullStr | Chemical constituents, antioxidant and antimicrobial activities of Pterygota alata (Roxb.) leaves extracts grown in Egypt |
| title_full_unstemmed | Chemical constituents, antioxidant and antimicrobial activities of Pterygota alata (Roxb.) leaves extracts grown in Egypt |
| title_short | Chemical constituents, antioxidant and antimicrobial activities of Pterygota alata (Roxb.) leaves extracts grown in Egypt |
| title_sort | chemical constituents antioxidant and antimicrobial activities of pterygota alata roxb leaves extracts grown in egypt |
| topic | pterygota alata (roxb.) phenolics antioxidant antimicrobial activities |
| url | https://nrmj.journals.ekb.eg/article_37210_9ab2a6b8dcefd58767104e201c74c776.pdf |
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