Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse
Summary: In the mouse retina, more than 30 retinal ganglion cell (RGC) subtypes have been classified based on a combined metric of morphological and functional characteristics. RGCs arise from a common pool of retinal progenitor cells during embryonic stages and differentiate into mature subtypes in...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-04-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124719304164 |
| _version_ | 1819059949761724416 |
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| author | Takae Kiyama Ye Long Ching-Kang Chen Christopher M. Whitaker Allison Shay Hongyu Wu Tudor C. Badea Amir Mohsenin Jan Parker-Thornburg William H. Klein Stephen L. Mills Stephen C. Massey Chai-An Mao |
| author_facet | Takae Kiyama Ye Long Ching-Kang Chen Christopher M. Whitaker Allison Shay Hongyu Wu Tudor C. Badea Amir Mohsenin Jan Parker-Thornburg William H. Klein Stephen L. Mills Stephen C. Massey Chai-An Mao |
| author_sort | Takae Kiyama |
| collection | DOAJ |
| description | Summary: In the mouse retina, more than 30 retinal ganglion cell (RGC) subtypes have been classified based on a combined metric of morphological and functional characteristics. RGCs arise from a common pool of retinal progenitor cells during embryonic stages and differentiate into mature subtypes in adult retinas. However, the cellular and molecular mechanisms controlling formation and maturation of such remarkable cellular diversity remain unknown. Here, we demonstrate that T-box transcription factor T-brain 1 (Tbr1) is expressed in two groups of morphologically and functionally distinct RGCs: the orientation-selective J-RGCs and a group of OFF-sustained RGCs with symmetrical dendritic arbors. When Tbr1 is genetically ablated during retinal development, these two RGC groups cannot develop. Ectopically expressing Tbr1 in M4 ipRGCs during development alters dendritic branching and density but not the inner plexiform layer stratification level. Our data indicate that Tbr1 plays critical roles in regulating the formation and dendritic morphogenesis of specific RGC types. : Little is known about how diversified retinal ganglion cell (RGC) subtypes develop. Using genetic and electrophysiological analyses, Kiyama et al. identify Tbr1 expression in two types of morphologically and functionally distinct OFF RGCs. Loss-of-function and gain-of-function studies show Tbr1 regulates the formation and dendritic morphogenesis of these cells. Keywords: Tbr1, RGC development, RGC subtype, J-RGC, Jam2, OFF-sustained RGC, OFF RGC, dendritic branching, dendritic morphogenesis |
| first_indexed | 2024-12-21T14:19:14Z |
| format | Article |
| id | doaj.art-9c325aa44e9d4b08967f1de839af1fac |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-21T14:19:14Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-9c325aa44e9d4b08967f1de839af1fac2022-12-21T19:00:50ZengElsevierCell Reports2211-12472019-04-01273900915.e5Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in MouseTakae Kiyama0Ye Long1Ching-Kang Chen2Christopher M. Whitaker3Allison Shay4Hongyu Wu5Tudor C. Badea6Amir Mohsenin7Jan Parker-Thornburg8William H. Klein9Stephen L. Mills10Stephen C. Massey11Chai-An Mao12Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX 77030, USARuiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX 77030, USADepartment of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USARuiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX 77030, USADepartment of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USARuiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX 77030, USANational Eye Institute, NIH, Bethesda, MD 20892, USARuiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX 77030, USA; Robert Cizik Eye Clinic, Houston, TX 77030, USADepartment of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USADepartment of Systems Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USARuiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX 77030, USARuiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX 77030, USARuiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX 77030, USA; Corresponding authorSummary: In the mouse retina, more than 30 retinal ganglion cell (RGC) subtypes have been classified based on a combined metric of morphological and functional characteristics. RGCs arise from a common pool of retinal progenitor cells during embryonic stages and differentiate into mature subtypes in adult retinas. However, the cellular and molecular mechanisms controlling formation and maturation of such remarkable cellular diversity remain unknown. Here, we demonstrate that T-box transcription factor T-brain 1 (Tbr1) is expressed in two groups of morphologically and functionally distinct RGCs: the orientation-selective J-RGCs and a group of OFF-sustained RGCs with symmetrical dendritic arbors. When Tbr1 is genetically ablated during retinal development, these two RGC groups cannot develop. Ectopically expressing Tbr1 in M4 ipRGCs during development alters dendritic branching and density but not the inner plexiform layer stratification level. Our data indicate that Tbr1 plays critical roles in regulating the formation and dendritic morphogenesis of specific RGC types. : Little is known about how diversified retinal ganglion cell (RGC) subtypes develop. Using genetic and electrophysiological analyses, Kiyama et al. identify Tbr1 expression in two types of morphologically and functionally distinct OFF RGCs. Loss-of-function and gain-of-function studies show Tbr1 regulates the formation and dendritic morphogenesis of these cells. Keywords: Tbr1, RGC development, RGC subtype, J-RGC, Jam2, OFF-sustained RGC, OFF RGC, dendritic branching, dendritic morphogenesishttp://www.sciencedirect.com/science/article/pii/S2211124719304164 |
| spellingShingle | Takae Kiyama Ye Long Ching-Kang Chen Christopher M. Whitaker Allison Shay Hongyu Wu Tudor C. Badea Amir Mohsenin Jan Parker-Thornburg William H. Klein Stephen L. Mills Stephen C. Massey Chai-An Mao Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse Cell Reports |
| title | Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse |
| title_full | Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse |
| title_fullStr | Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse |
| title_full_unstemmed | Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse |
| title_short | Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse |
| title_sort | essential roles of tbr1 in the formation and maintenance of the orientation selective j rgcs and a group of off sustained rgcs in mouse |
| url | http://www.sciencedirect.com/science/article/pii/S2211124719304164 |
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