A Method for Simultaneous Determination of 20 Fusarium Toxins in Cereals by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry with a Pentafluorophenyl Column
A high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) method was developed for simultaneous determination of 20 Fusarium toxins (nivalenol, fusarenon-X, deoxynivalenol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, HT-2 toxin, T-2 toxin, neosolaniol, diacetoxyscirp...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-05-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/7/5/1664 |
| _version_ | 1811185936906584064 |
|---|---|
| author | Masayoshi Tamura Naoki Mochizuki Yasushi Nagatomi Koichi Harayama Akira Toriba Kazuichi Hayakawa |
| author_facet | Masayoshi Tamura Naoki Mochizuki Yasushi Nagatomi Koichi Harayama Akira Toriba Kazuichi Hayakawa |
| author_sort | Masayoshi Tamura |
| collection | DOAJ |
| description | A high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) method was developed for simultaneous determination of 20 Fusarium toxins (nivalenol, fusarenon-X, deoxynivalenol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, HT-2 toxin, T-2 toxin, neosolaniol, diacetoxyscirpenol, fumonisin B1, fumonisin B2, fumonisin B3, fumonisin A1, fumonisin A2, fumonisin A3, zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol) in cereals. The separation of 20 Fusarium toxins with good peak shapes was achieved using a pentafluorophenyl column, and Orbitrap MS was able to detect accurately from cereal matrix components within ±0.77 ppm. The samples were prepared using a QuEChERS kit for extraction and a multifunctional cartridge for purification. The linearity, repeatability, and recovery of the method were >0.9964, 0.8%–14.7%, and 71%–106%, respectively. Using this method, an analysis of 34 commercially available cereals detected the presence of deoxynivalenol, 15-acetyl deoxynivalenol, fumonisin B1, fumonisin B2, fumonisin B3, fumonisn A1, fumonisin A2, fumonisin A3, and zearalenone in corn samples with high concentration and frequency. Trichothecenes was detected from wheat samples with high frequency; in particular, the concentration of deoxynivalenol was high. Conversely, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol were not detected in any of the samples. |
| first_indexed | 2024-04-11T13:38:59Z |
| format | Article |
| id | doaj.art-9c329e97d5af441098f0c28eb828e7a7 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T13:38:59Z |
| publishDate | 2015-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-9c329e97d5af441098f0c28eb828e7a72022-12-22T04:21:23ZengMDPI AGToxins2072-66512015-05-01751664168210.3390/toxins7051664toxins7051664A Method for Simultaneous Determination of 20 Fusarium Toxins in Cereals by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry with a Pentafluorophenyl ColumnMasayoshi Tamura0Naoki Mochizuki1Yasushi Nagatomi2Koichi Harayama3Akira Toriba4Kazuichi Hayakawa5Research Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, JapanResearch & Development Center, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, JapanResearch Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, JapanResearch Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, JapanInstitute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa-shi, Ishikawa 920-1192, JapanInstitute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa-shi, Ishikawa 920-1192, JapanA high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) method was developed for simultaneous determination of 20 Fusarium toxins (nivalenol, fusarenon-X, deoxynivalenol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, HT-2 toxin, T-2 toxin, neosolaniol, diacetoxyscirpenol, fumonisin B1, fumonisin B2, fumonisin B3, fumonisin A1, fumonisin A2, fumonisin A3, zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol) in cereals. The separation of 20 Fusarium toxins with good peak shapes was achieved using a pentafluorophenyl column, and Orbitrap MS was able to detect accurately from cereal matrix components within ±0.77 ppm. The samples were prepared using a QuEChERS kit for extraction and a multifunctional cartridge for purification. The linearity, repeatability, and recovery of the method were >0.9964, 0.8%–14.7%, and 71%–106%, respectively. Using this method, an analysis of 34 commercially available cereals detected the presence of deoxynivalenol, 15-acetyl deoxynivalenol, fumonisin B1, fumonisin B2, fumonisin B3, fumonisn A1, fumonisin A2, fumonisin A3, and zearalenone in corn samples with high concentration and frequency. Trichothecenes was detected from wheat samples with high frequency; in particular, the concentration of deoxynivalenol was high. Conversely, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol were not detected in any of the samples.http://www.mdpi.com/2072-6651/7/5/1664Fusarium toxinsLC-Orbitrap MSpentafluorophenyl columnsimultaneous determinationcereals |
| spellingShingle | Masayoshi Tamura Naoki Mochizuki Yasushi Nagatomi Koichi Harayama Akira Toriba Kazuichi Hayakawa A Method for Simultaneous Determination of 20 Fusarium Toxins in Cereals by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry with a Pentafluorophenyl Column Toxins Fusarium toxins LC-Orbitrap MS pentafluorophenyl column simultaneous determination cereals |
| title | A Method for Simultaneous Determination of 20 Fusarium Toxins in Cereals by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry with a Pentafluorophenyl Column |
| title_full | A Method for Simultaneous Determination of 20 Fusarium Toxins in Cereals by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry with a Pentafluorophenyl Column |
| title_fullStr | A Method for Simultaneous Determination of 20 Fusarium Toxins in Cereals by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry with a Pentafluorophenyl Column |
| title_full_unstemmed | A Method for Simultaneous Determination of 20 Fusarium Toxins in Cereals by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry with a Pentafluorophenyl Column |
| title_short | A Method for Simultaneous Determination of 20 Fusarium Toxins in Cereals by High-Resolution Liquid Chromatography-Orbitrap Mass Spectrometry with a Pentafluorophenyl Column |
| title_sort | method for simultaneous determination of 20 fusarium toxins in cereals by high resolution liquid chromatography orbitrap mass spectrometry with a pentafluorophenyl column |
| topic | Fusarium toxins LC-Orbitrap MS pentafluorophenyl column simultaneous determination cereals |
| url | http://www.mdpi.com/2072-6651/7/5/1664 |
| work_keys_str_mv | AT masayoshitamura amethodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT naokimochizuki amethodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT yasushinagatomi amethodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT koichiharayama amethodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT akiratoriba amethodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT kazuichihayakawa amethodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT masayoshitamura methodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT naokimochizuki methodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT yasushinagatomi methodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT koichiharayama methodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT akiratoriba methodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn AT kazuichihayakawa methodforsimultaneousdeterminationof20fusariumtoxinsincerealsbyhighresolutionliquidchromatographyorbitrapmassspectrometrywithapentafluorophenylcolumn |