Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum
IntroductionPhycocyanin offers advantageous biological effects, including immune-regulatory, anticancer, antioxidant, and anti-inflammation capabilities. While PC, as a natural pigment molecule, is different from synthetic pigment, it can be easily degradable under high temperature and light conditi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1181262/full |
| _version_ | 1797851385296846848 |
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| author | Ying Bai Xueting Li Yuqianqian Xie Yingzhen Wang Xiuping Dong Hang Qi |
| author_facet | Ying Bai Xueting Li Yuqianqian Xie Yingzhen Wang Xiuping Dong Hang Qi |
| author_sort | Ying Bai |
| collection | DOAJ |
| description | IntroductionPhycocyanin offers advantageous biological effects, including immune-regulatory, anticancer, antioxidant, and anti-inflammation capabilities. While PC, as a natural pigment molecule, is different from synthetic pigment, it can be easily degradable under high temperature and light conditions.MethodsIn this work, the impact of ultrasound treatment on the complex of PC and phlorotannin structural and functional characteristics was carefully investigated. The interaction between PC and phlorotannin after ultrasound treatment was studied by UV–Vis, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, fourier transform infrared (FTIR) spectroscopy. Additionally, the antioxidant potential and in vitro digestibility of the complexes were assessed.ResultsThe result was manifested as the UV–Vis spectrum reduction effect, fluorescence quenching effect and weak conformational change of the CD spectrum of PC. PC was identified as amorphous based on the X-ray diffraction (XRD) data and that phlorotannin was embedded into the PC matrix. The differential scanning calorimetry (DSC) results showed that ultrasound treatment and the addition of phlorotannin could improve the denaturation peak temperatures (Td) of PC to 78.7°C. In vitro digestion and free radical scavenging experiments showed that appropriate ultrasound treatment and the addition of phlorotannin were more resistant to simulated gastrointestinal conditions and could improve DPPH and ABTS+ free radical scavenging performance.DiscussionUltrasound treatment and the addition of phlorotannin changed the structural and functional properties of PC. These results demonstrated the feasibility of ultrasound-assisted phlorotannin from A. nodosum in improving the functional properties of PC and provided a possibility for the application of PC-polyphenol complexes as functional food ingredients or as bioactive materials. |
| first_indexed | 2024-04-09T19:17:02Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-09T19:17:02Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-9c3326d737bf4a859835d07b1b290d5f2023-04-06T04:49:42ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-04-011010.3389/fnut.2023.11812621181262Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosumYing Bai0Xueting Li1Yuqianqian Xie2Yingzhen Wang3Xiuping Dong4Hang Qi5School of Food Science and Technology, National Engineering Research Center of Seafood, Liaoning Provincial Aquatic Products Deep Processing Technology Research Center, Dalian Polytechnic University, Dalian, ChinaHaide College, Ocean University of China, Qingdao, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Liaoning Provincial Aquatic Products Deep Processing Technology Research Center, Dalian Polytechnic University, Dalian, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Liaoning Provincial Aquatic Products Deep Processing Technology Research Center, Dalian Polytechnic University, Dalian, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Liaoning Provincial Aquatic Products Deep Processing Technology Research Center, Dalian Polytechnic University, Dalian, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Liaoning Provincial Aquatic Products Deep Processing Technology Research Center, Dalian Polytechnic University, Dalian, ChinaIntroductionPhycocyanin offers advantageous biological effects, including immune-regulatory, anticancer, antioxidant, and anti-inflammation capabilities. While PC, as a natural pigment molecule, is different from synthetic pigment, it can be easily degradable under high temperature and light conditions.MethodsIn this work, the impact of ultrasound treatment on the complex of PC and phlorotannin structural and functional characteristics was carefully investigated. The interaction between PC and phlorotannin after ultrasound treatment was studied by UV–Vis, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, fourier transform infrared (FTIR) spectroscopy. Additionally, the antioxidant potential and in vitro digestibility of the complexes were assessed.ResultsThe result was manifested as the UV–Vis spectrum reduction effect, fluorescence quenching effect and weak conformational change of the CD spectrum of PC. PC was identified as amorphous based on the X-ray diffraction (XRD) data and that phlorotannin was embedded into the PC matrix. The differential scanning calorimetry (DSC) results showed that ultrasound treatment and the addition of phlorotannin could improve the denaturation peak temperatures (Td) of PC to 78.7°C. In vitro digestion and free radical scavenging experiments showed that appropriate ultrasound treatment and the addition of phlorotannin were more resistant to simulated gastrointestinal conditions and could improve DPPH and ABTS+ free radical scavenging performance.DiscussionUltrasound treatment and the addition of phlorotannin changed the structural and functional properties of PC. These results demonstrated the feasibility of ultrasound-assisted phlorotannin from A. nodosum in improving the functional properties of PC and provided a possibility for the application of PC-polyphenol complexes as functional food ingredients or as bioactive materials.https://www.frontiersin.org/articles/10.3389/fnut.2023.1181262/fullultrasoundphycocyaninphlorotanninAscophyllum nodosuminteractionmodification |
| spellingShingle | Ying Bai Xueting Li Yuqianqian Xie Yingzhen Wang Xiuping Dong Hang Qi Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum Frontiers in Nutrition ultrasound phycocyanin phlorotannin Ascophyllum nodosum interaction modification |
| title | Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum |
| title_full | Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum |
| title_fullStr | Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum |
| title_full_unstemmed | Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum |
| title_short | Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum |
| title_sort | ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from ascophyllum nodosum |
| topic | ultrasound phycocyanin phlorotannin Ascophyllum nodosum interaction modification |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1181262/full |
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