Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta
Objective·To explore the feasibility of fluorinated decellularized rabbit aorta as a small artificial blood vessel for tissue engineering.Methods·The obtained rabbit aorta was decellularized in combination with Triton X-100, sodium deoxycholate (SD), sodium dodecyl sulfate (SDS), DNAse, and RNAse. H...
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Editorial Office of Journal of Shanghai Jiao Tong University (Medical Science)
2023-07-01
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| Series: | Shanghai Jiaotong Daxue xuebao. Yixue ban |
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| Online Access: | https://xuebao.shsmu.edu.cn/article/2023/1674-8115/1674-8115-2023-43-7-814.shtml |
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| author | ZHANG Rujie ZHOU Guangdong WANG Jianbo WENG Guangdong LEI Dong GONG Wenhui |
| author_facet | ZHANG Rujie ZHOU Guangdong WANG Jianbo WENG Guangdong LEI Dong GONG Wenhui |
| author_sort | ZHANG Rujie |
| collection | DOAJ |
| description | Objective·To explore the feasibility of fluorinated decellularized rabbit aorta as a small artificial blood vessel for tissue engineering.Methods·The obtained rabbit aorta was decellularized in combination with Triton X-100, sodium deoxycholate (SD), sodium dodecyl sulfate (SDS), DNAse, and RNAse. Hematoxylin-eosin staining (H-E staining), Masson staining and Verhoff-von Gieson staining were performed in the decellularized group and undecellularized group, respectively. The effect of decellularization was identified by field emission scanning electron microscope, and the morphological changes of decellularized blood vessels were observed. The decellularized rabbit aorta was used as the arterialized artificial small vessel scaffold, and the decellularized small vessel intima was modified with liquid perfluorocarbons coating to prepare a new type of artificial small vessel. The characteristic groups of the artificial small vessel were qualitatively and quantitatively determined. The dissipation time of liquid on the inner surface of the vessel and the flow of liquid on the surface of the vessel tilted at 45° were observed to analyze the hydrophobicity of the vessel. The blood vessels in the decellularized group and the fluorinated group were implanted with platelet-rich plasma, incubated, and observed under an electron microscope to evaluate the antiplatelet aggregation in vitro. The balloon pressure pump was connected to the aorta of the undecellularized group, decellularized group and fluoride group for bursting pressure test.Results·Histological observation of blood vessels showed that the combination could effectively remove cells while retaining collagen and elastic fibers, and there was no damage to the intima under the electron microscope. There was no significant difference in the pressure blasting test among the three groups. In the hydrophobicity experiment, the retention time of water droplets on the membrane of the fluorinated group was over 5 min, and no obvious water marks were left on the 45° inclined plate. In the platelet adhesion test, intimal aggregation activated platelets in the decellularized group, while they were inhibited in the fluorinated group.Conclusion·The decellularized blood vessels have good mechanical properties and physical stability by combined decellularization, and the fluorinated coating makes the blood vessels have good anticoagulant and biocompatibility. |
| first_indexed | 2024-03-08T00:02:15Z |
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| id | doaj.art-9c3465e4c2014be4a57a766efba998e5 |
| institution | Directory Open Access Journal |
| issn | 1674-8115 |
| language | zho |
| last_indexed | 2024-03-08T00:02:15Z |
| publishDate | 2023-07-01 |
| publisher | Editorial Office of Journal of Shanghai Jiao Tong University (Medical Science) |
| record_format | Article |
| series | Shanghai Jiaotong Daxue xuebao. Yixue ban |
| spelling | doaj.art-9c3465e4c2014be4a57a766efba998e52024-02-18T02:20:07ZzhoEditorial Office of Journal of Shanghai Jiao Tong University (Medical Science)Shanghai Jiaotong Daxue xuebao. Yixue ban1674-81152023-07-0143781482010.3969/j.issn.1674-8115.2023.07.0031674-8115（2023）07-0814-07Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aortaZHANG Rujie0ZHOU Guangdong1WANG Jianbo2WENG Guangdong3LEI Dong4GONG Wenhui5Department of Cardio-Vascular Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230088, ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai 9th People′s Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Lab of Tissue Engineering, Shanghai 200125, ChinaDepartment of Cardio-Vascular Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230088, ChinaDepartment of Cardio-Vascular Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230088, ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai 9th People′s Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Lab of Tissue Engineering, Shanghai 200125, ChinaDepartment of Cardio-Vascular Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230088, ChinaObjective·To explore the feasibility of fluorinated decellularized rabbit aorta as a small artificial blood vessel for tissue engineering.Methods·The obtained rabbit aorta was decellularized in combination with Triton X-100, sodium deoxycholate (SD), sodium dodecyl sulfate (SDS), DNAse, and RNAse. Hematoxylin-eosin staining (H-E staining), Masson staining and Verhoff-von Gieson staining were performed in the decellularized group and undecellularized group, respectively. The effect of decellularization was identified by field emission scanning electron microscope, and the morphological changes of decellularized blood vessels were observed. The decellularized rabbit aorta was used as the arterialized artificial small vessel scaffold, and the decellularized small vessel intima was modified with liquid perfluorocarbons coating to prepare a new type of artificial small vessel. The characteristic groups of the artificial small vessel were qualitatively and quantitatively determined. The dissipation time of liquid on the inner surface of the vessel and the flow of liquid on the surface of the vessel tilted at 45° were observed to analyze the hydrophobicity of the vessel. The blood vessels in the decellularized group and the fluorinated group were implanted with platelet-rich plasma, incubated, and observed under an electron microscope to evaluate the antiplatelet aggregation in vitro. The balloon pressure pump was connected to the aorta of the undecellularized group, decellularized group and fluoride group for bursting pressure test.Results·Histological observation of blood vessels showed that the combination could effectively remove cells while retaining collagen and elastic fibers, and there was no damage to the intima under the electron microscope. There was no significant difference in the pressure blasting test among the three groups. In the hydrophobicity experiment, the retention time of water droplets on the membrane of the fluorinated group was over 5 min, and no obvious water marks were left on the 45° inclined plate. In the platelet adhesion test, intimal aggregation activated platelets in the decellularized group, while they were inhibited in the fluorinated group.Conclusion·The decellularized blood vessels have good mechanical properties and physical stability by combined decellularization, and the fluorinated coating makes the blood vessels have good anticoagulant and biocompatibility.https://xuebao.shsmu.edu.cn/article/2023/1674-8115/1674-8115-2023-43-7-814.shtmldecellularizationtissue engineeringrabbitsmall blood vesselintimacoating |
| spellingShingle | ZHANG Rujie ZHOU Guangdong WANG Jianbo WENG Guangdong LEI Dong GONG Wenhui Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta Shanghai Jiaotong Daxue xuebao. Yixue ban decellularization tissue engineering rabbit small blood vessel intima coating |
| title | Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta |
| title_full | Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta |
| title_fullStr | Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta |
| title_full_unstemmed | Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta |
| title_short | Feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta |
| title_sort | feasibility study on the preparation of artificial small blood vessel by fluorinated decellularized rabbit aorta |
| topic | decellularization tissue engineering rabbit small blood vessel intima coating |
| url | https://xuebao.shsmu.edu.cn/article/2023/1674-8115/1674-8115-2023-43-7-814.shtml |
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