The in vitro antitrypanosomal activity of Albizia gummifera leaf extracts
For the control and treatment of trypanosomiasis, a limited number of chemotherapeutic drugs with mild side effects are available. As a result, a quest for a less toxic herbal treatment for trypanosomiasis is needed. Ethanolic extract of A. gummifera leaf (EEAL) and aqueous extract of Albizia. gummi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2021-07-01
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| Series: | Open Veterinary Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/ovs-2020-0105 |
| _version_ | 1819178037862727680 |
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| author | Oloruntola Deborah Adebukola Dada Ebenezer Oluyemi Oladunmoye Muftau Kolawole |
| author_facet | Oloruntola Deborah Adebukola Dada Ebenezer Oluyemi Oladunmoye Muftau Kolawole |
| author_sort | Oloruntola Deborah Adebukola |
| collection | DOAJ |
| description | For the control and treatment of trypanosomiasis, a limited number of chemotherapeutic drugs with mild side effects are available. As a result, a quest for a less toxic herbal treatment for trypanosomiasis is needed. Ethanolic extract of A. gummifera leaf (EEAL) and aqueous extract of Albizia. gummifera leaf (AEAL) were tested for antitrypanosomal activity against Trypanosoma brucei brucei in vitro. We first compared the phytochemical concentrations of EEAL and AEAL and discovered that EEAL had higher phytochemical concentrations on average than AEAL: flavonoids (4.26 mg/g vs 2.50 mg/g); alkaloids (38.40 mg/g vs 19.80 mg/g); tannins (230.7 mg/g vs 45.74 mg/g) and saponins (128.66 vs 44.33g/g). From the result of phytochemical concentrations of the two compounds, the higher values observed in flavonoids and alkaloid of EEAL led us to hypothesize that EEAL would have greater trypanocidal activity. Following that, EEAL and AEAL were tested for antitrypanosomal activity in vitro. Forty µl of blood holding in about 25±8 parasites/field was mixed with 20 µl of the EEAL and AEAL solutions of 100, 80, 60 mg/ml to produce an efficacious test concentration of 25, 20 and 15 mg/ml, sequentially. The extracts inhibited parasite motility and eliminated the organisms at the concentrations used in vitro, except for 15 mg/ml AEAL and 20 mg/ml AEAL. Following the screening, the Albizia gummifera ethanolic extract found to have positive in vitro trypanocidal activity. More research is needed to determine the concentrations of the extract for the in vivo test. |
| first_indexed | 2024-12-22T21:36:11Z |
| format | Article |
| id | doaj.art-9c3b6dcbe6664c419c4b569272cbdfd0 |
| institution | Directory Open Access Journal |
| issn | 2544-8951 |
| language | English |
| last_indexed | 2024-12-22T21:36:11Z |
| publishDate | 2021-07-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Veterinary Science |
| spelling | doaj.art-9c3b6dcbe6664c419c4b569272cbdfd02022-12-21T18:11:44ZengDe GruyterOpen Veterinary Science2544-89512021-07-0121333910.1515/ovs-2020-0105The in vitro antitrypanosomal activity of Albizia gummifera leaf extractsOloruntola Deborah Adebukola0Dada Ebenezer Oluyemi1Oladunmoye Muftau Kolawole2Department of Microbiology, The Federal University of Technology, Akure, Nigeria.Department of Microbiology, The Federal University of Technology, Akure, Nigeria.Department of Microbiology, The Federal University of Technology, Akure, Nigeria.For the control and treatment of trypanosomiasis, a limited number of chemotherapeutic drugs with mild side effects are available. As a result, a quest for a less toxic herbal treatment for trypanosomiasis is needed. Ethanolic extract of A. gummifera leaf (EEAL) and aqueous extract of Albizia. gummifera leaf (AEAL) were tested for antitrypanosomal activity against Trypanosoma brucei brucei in vitro. We first compared the phytochemical concentrations of EEAL and AEAL and discovered that EEAL had higher phytochemical concentrations on average than AEAL: flavonoids (4.26 mg/g vs 2.50 mg/g); alkaloids (38.40 mg/g vs 19.80 mg/g); tannins (230.7 mg/g vs 45.74 mg/g) and saponins (128.66 vs 44.33g/g). From the result of phytochemical concentrations of the two compounds, the higher values observed in flavonoids and alkaloid of EEAL led us to hypothesize that EEAL would have greater trypanocidal activity. Following that, EEAL and AEAL were tested for antitrypanosomal activity in vitro. Forty µl of blood holding in about 25±8 parasites/field was mixed with 20 µl of the EEAL and AEAL solutions of 100, 80, 60 mg/ml to produce an efficacious test concentration of 25, 20 and 15 mg/ml, sequentially. The extracts inhibited parasite motility and eliminated the organisms at the concentrations used in vitro, except for 15 mg/ml AEAL and 20 mg/ml AEAL. Following the screening, the Albizia gummifera ethanolic extract found to have positive in vitro trypanocidal activity. More research is needed to determine the concentrations of the extract for the in vivo test.https://doi.org/10.1515/ovs-2020-0105bioactive compoundsherbal extractsphytochemicalstrypanosomiasis |
| spellingShingle | Oloruntola Deborah Adebukola Dada Ebenezer Oluyemi Oladunmoye Muftau Kolawole The in vitro antitrypanosomal activity of Albizia gummifera leaf extracts Open Veterinary Science bioactive compounds herbal extracts phytochemicals trypanosomiasis |
| title | The in vitro antitrypanosomal activity of Albizia gummifera leaf extracts |
| title_full | The in vitro antitrypanosomal activity of Albizia gummifera leaf extracts |
| title_fullStr | The in vitro antitrypanosomal activity of Albizia gummifera leaf extracts |
| title_full_unstemmed | The in vitro antitrypanosomal activity of Albizia gummifera leaf extracts |
| title_short | The in vitro antitrypanosomal activity of Albizia gummifera leaf extracts |
| title_sort | in vitro antitrypanosomal activity of albizia gummifera leaf extracts |
| topic | bioactive compounds herbal extracts phytochemicals trypanosomiasis |
| url | https://doi.org/10.1515/ovs-2020-0105 |
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