Treatment with the Probiotic Product Aviguard<sup>®</sup> Alleviates Inflammatory Responses during <i>Campylobacter jejuni</i>-Induced Acute Enterocolitis in Mice
Prevalences of <i>Campylobacter (C.) jejuni</i> infections are progressively rising globally. Given that probiotic feed additives, such as the commercial product Aviguard<sup>®</sup>, have been shown to be effective in reducing enteropathogens, such as <i>Salmonella<...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/13/6683 |
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| author | Markus M. Heimesaat Dennis Weschka Soraya Mousavi Stefan Bereswill |
| author_facet | Markus M. Heimesaat Dennis Weschka Soraya Mousavi Stefan Bereswill |
| author_sort | Markus M. Heimesaat |
| collection | DOAJ |
| description | Prevalences of <i>Campylobacter (C.) jejuni</i> infections are progressively rising globally. Given that probiotic feed additives, such as the commercial product Aviguard<sup>®</sup>, have been shown to be effective in reducing enteropathogens, such as <i>Salmonella</i>, in vertebrates, including livestock, we assessed potential anti-pathogenic and immune-modulatory properties of Aviguard<sup>®</sup> during acute <i>C. jejuni</i>-induced murine enterocolitis. Therefore, microbiota-depleted IL-10<sup>−/−</sup> mice were infected with <i>C. jejuni</i> strain 81-176 by gavage and orally treated with Aviguard<sup>®</sup> or placebo from day 2 to 4 post-infection. The applied probiotic bacteria could be rescued from the intestinal tract of treated mice, but with lower obligate anaerobic bacterial counts in <i>C. jejuni</i>-infected as compared to non-infected mice. Whereas comparable gastrointestinal pathogen loads could be detected in both groups until day 6 post-infection, Aviguard<sup>®</sup> treatment resulted in improved clinical outcome and attenuated apoptotic cell responses in infected large intestines during acute campylobacteriosis. Furthermore, less distinct pro-inflammatory immune responses could be observed not only in the intestinal tract, but also in extra-intestinal compartments on day 6 post-infection. In conclusion, we show here for the first time that Aviguard<sup>®</sup> exerts potent disease-alleviating effects in acute <i>C. jejuni</i>-induced murine enterocolitis and might be a promising probiotic treatment option for severe campylobacteriosis in humans. |
| first_indexed | 2024-03-10T10:10:51Z |
| format | Article |
| id | doaj.art-9c3ce443fec0434081eb08afde461e6f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:10:51Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9c3ce443fec0434081eb08afde461e6f2023-11-22T01:12:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012213668310.3390/ijms22136683Treatment with the Probiotic Product Aviguard<sup>®</sup> Alleviates Inflammatory Responses during <i>Campylobacter jejuni</i>-Induced Acute Enterocolitis in MiceMarkus M. Heimesaat0Dennis Weschka1Soraya Mousavi2Stefan Bereswill3Institute of Microbiology, Infectious Diseases and Immunology, Gastrointestinal Microbiology Research Group, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyInstitute of Microbiology, Infectious Diseases and Immunology, Gastrointestinal Microbiology Research Group, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyInstitute of Microbiology, Infectious Diseases and Immunology, Gastrointestinal Microbiology Research Group, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyInstitute of Microbiology, Infectious Diseases and Immunology, Gastrointestinal Microbiology Research Group, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, GermanyPrevalences of <i>Campylobacter (C.) jejuni</i> infections are progressively rising globally. Given that probiotic feed additives, such as the commercial product Aviguard<sup>®</sup>, have been shown to be effective in reducing enteropathogens, such as <i>Salmonella</i>, in vertebrates, including livestock, we assessed potential anti-pathogenic and immune-modulatory properties of Aviguard<sup>®</sup> during acute <i>C. jejuni</i>-induced murine enterocolitis. Therefore, microbiota-depleted IL-10<sup>−/−</sup> mice were infected with <i>C. jejuni</i> strain 81-176 by gavage and orally treated with Aviguard<sup>®</sup> or placebo from day 2 to 4 post-infection. The applied probiotic bacteria could be rescued from the intestinal tract of treated mice, but with lower obligate anaerobic bacterial counts in <i>C. jejuni</i>-infected as compared to non-infected mice. Whereas comparable gastrointestinal pathogen loads could be detected in both groups until day 6 post-infection, Aviguard<sup>®</sup> treatment resulted in improved clinical outcome and attenuated apoptotic cell responses in infected large intestines during acute campylobacteriosis. Furthermore, less distinct pro-inflammatory immune responses could be observed not only in the intestinal tract, but also in extra-intestinal compartments on day 6 post-infection. In conclusion, we show here for the first time that Aviguard<sup>®</sup> exerts potent disease-alleviating effects in acute <i>C. jejuni</i>-induced murine enterocolitis and might be a promising probiotic treatment option for severe campylobacteriosis in humans.https://www.mdpi.com/1422-0067/22/13/6683competitive exclusion productAviguard<sup>®</sup>gut microbiotaenteropathogenic infection<i>Campylobacter jejuni</i>immune-modulatory effects |
| spellingShingle | Markus M. Heimesaat Dennis Weschka Soraya Mousavi Stefan Bereswill Treatment with the Probiotic Product Aviguard<sup>®</sup> Alleviates Inflammatory Responses during <i>Campylobacter jejuni</i>-Induced Acute Enterocolitis in Mice International Journal of Molecular Sciences competitive exclusion product Aviguard<sup>®</sup> gut microbiota enteropathogenic infection <i>Campylobacter jejuni</i> immune-modulatory effects |
| title | Treatment with the Probiotic Product Aviguard<sup>®</sup> Alleviates Inflammatory Responses during <i>Campylobacter jejuni</i>-Induced Acute Enterocolitis in Mice |
| title_full | Treatment with the Probiotic Product Aviguard<sup>®</sup> Alleviates Inflammatory Responses during <i>Campylobacter jejuni</i>-Induced Acute Enterocolitis in Mice |
| title_fullStr | Treatment with the Probiotic Product Aviguard<sup>®</sup> Alleviates Inflammatory Responses during <i>Campylobacter jejuni</i>-Induced Acute Enterocolitis in Mice |
| title_full_unstemmed | Treatment with the Probiotic Product Aviguard<sup>®</sup> Alleviates Inflammatory Responses during <i>Campylobacter jejuni</i>-Induced Acute Enterocolitis in Mice |
| title_short | Treatment with the Probiotic Product Aviguard<sup>®</sup> Alleviates Inflammatory Responses during <i>Campylobacter jejuni</i>-Induced Acute Enterocolitis in Mice |
| title_sort | treatment with the probiotic product aviguard sup r sup alleviates inflammatory responses during i campylobacter jejuni i induced acute enterocolitis in mice |
| topic | competitive exclusion product Aviguard<sup>®</sup> gut microbiota enteropathogenic infection <i>Campylobacter jejuni</i> immune-modulatory effects |
| url | https://www.mdpi.com/1422-0067/22/13/6683 |
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