The Diversity of Bacteria Associated with the Invasive Gall Wasp <em>Dryocosmus kuriphilus</em>, Its Galls and a Specialist Parasitoid on Chestnuts
<i>Dryocosmus kuriphilus</i> (Hymenoptera: Cynipidae) induces galls on chestnut trees, which results in massive yield losses worldwide. <i>Torymus sinensis</i> (Hymenoptera: Torymidae) is a host-specific parasitoid that phenologically synchronizes with <i>D. kuriphilus&...
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MDPI AG
2022-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/1/86 |
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| author | Xiaohui Yang Yu Hui Daohong Zhu Yang Zeng Lvquan Zhao Xuemei Yang Yumei Wang |
| author_facet | Xiaohui Yang Yu Hui Daohong Zhu Yang Zeng Lvquan Zhao Xuemei Yang Yumei Wang |
| author_sort | Xiaohui Yang |
| collection | DOAJ |
| description | <i>Dryocosmus kuriphilus</i> (Hymenoptera: Cynipidae) induces galls on chestnut trees, which results in massive yield losses worldwide. <i>Torymus sinensis</i> (Hymenoptera: Torymidae) is a host-specific parasitoid that phenologically synchronizes with <i>D. kuriphilus</i>. Bacteria play important roles in the life cycle of galling insects. The aim of this research is to investigate the bacterial communities and predominant bacteria of <i>D. kuriphilus</i>, <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and the galled twigs of <i>Castanea mollissima</i>. We sequenced the V5–V7 region of the bacterial 16S ribosomal RNA in <i>D. kuriphilus</i>, <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and galled twigs using high-throughput sequencing for the first time. We provide the first evidence that <i>D. kuriphilus</i> shares most bacterial species with <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and galled twigs. The predominant bacteria of <i>D. kuriphilus</i> are <i>Serratia</i> sp. and <i>Pseudomonas</i> sp. Furthermore, the bacterial community structures of <i>D. kuriphilus</i> and <i>T. sinensis</i> clearly differ from those of the other groups. Many species of the <i>Serratia</i> and <i>Pseudomonas</i> genera are plant pathogenic bacteria, and we suggest that <i>D. kuriphilus</i> may be a potential vector of plant pathogens. Furthermore, a total of 111 bacteria are common to <i>D. kuriphilus</i> adults, <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and galled twigs, and we suggest that the bacteria may transmit horizontally among <i>D. kuriphilus</i>, <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and galled twigs on the basis of their ecological associations. |
| first_indexed | 2024-03-10T01:15:24Z |
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| institution | Directory Open Access Journal |
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| spelling | doaj.art-9c41330382364e40b0891eae89603a472023-11-23T14:10:35ZengMDPI AGInsects2075-44502022-01-011318610.3390/insects13010086The Diversity of Bacteria Associated with the Invasive Gall Wasp <em>Dryocosmus kuriphilus</em>, Its Galls and a Specialist Parasitoid on ChestnutsXiaohui Yang0Yu Hui1Daohong Zhu2Yang Zeng3Lvquan Zhao4Xuemei Yang5Yumei Wang6College of Life Science, Hunan Normal University, Changsha 410081, ChinaCollege of Life Science, Hunan Normal University, Changsha 410081, ChinaLaboratory of Insect Behavior and Evolutionary Ecology, Central South University of Forestry and Technology, Changsha 410004, ChinaLaboratory of Insect Behavior and Evolutionary Ecology, Central South University of Forestry and Technology, Changsha 410004, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Life Science, Hunan Normal University, Changsha 410081, ChinaCollege of Life Science, Hunan Normal University, Changsha 410081, China<i>Dryocosmus kuriphilus</i> (Hymenoptera: Cynipidae) induces galls on chestnut trees, which results in massive yield losses worldwide. <i>Torymus sinensis</i> (Hymenoptera: Torymidae) is a host-specific parasitoid that phenologically synchronizes with <i>D. kuriphilus</i>. Bacteria play important roles in the life cycle of galling insects. The aim of this research is to investigate the bacterial communities and predominant bacteria of <i>D. kuriphilus</i>, <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and the galled twigs of <i>Castanea mollissima</i>. We sequenced the V5–V7 region of the bacterial 16S ribosomal RNA in <i>D. kuriphilus</i>, <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and galled twigs using high-throughput sequencing for the first time. We provide the first evidence that <i>D. kuriphilus</i> shares most bacterial species with <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and galled twigs. The predominant bacteria of <i>D. kuriphilus</i> are <i>Serratia</i> sp. and <i>Pseudomonas</i> sp. Furthermore, the bacterial community structures of <i>D. kuriphilus</i> and <i>T. sinensis</i> clearly differ from those of the other groups. Many species of the <i>Serratia</i> and <i>Pseudomonas</i> genera are plant pathogenic bacteria, and we suggest that <i>D. kuriphilus</i> may be a potential vector of plant pathogens. Furthermore, a total of 111 bacteria are common to <i>D. kuriphilus</i> adults, <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and galled twigs, and we suggest that the bacteria may transmit horizontally among <i>D. kuriphilus</i>, <i>T. sinensis</i>, <i>D. kuriphilus</i> galls and galled twigs on the basis of their ecological associations.https://www.mdpi.com/2075-4450/13/1/86<i>Dryocosmus kuriphilus</i><i>Torymus sinensis</i>diversitybacterial communitygallshigh-throughput sequencing |
| spellingShingle | Xiaohui Yang Yu Hui Daohong Zhu Yang Zeng Lvquan Zhao Xuemei Yang Yumei Wang The Diversity of Bacteria Associated with the Invasive Gall Wasp <em>Dryocosmus kuriphilus</em>, Its Galls and a Specialist Parasitoid on Chestnuts Insects <i>Dryocosmus kuriphilus</i> <i>Torymus sinensis</i> diversity bacterial community galls high-throughput sequencing |
| title | The Diversity of Bacteria Associated with the Invasive Gall Wasp <em>Dryocosmus kuriphilus</em>, Its Galls and a Specialist Parasitoid on Chestnuts |
| title_full | The Diversity of Bacteria Associated with the Invasive Gall Wasp <em>Dryocosmus kuriphilus</em>, Its Galls and a Specialist Parasitoid on Chestnuts |
| title_fullStr | The Diversity of Bacteria Associated with the Invasive Gall Wasp <em>Dryocosmus kuriphilus</em>, Its Galls and a Specialist Parasitoid on Chestnuts |
| title_full_unstemmed | The Diversity of Bacteria Associated with the Invasive Gall Wasp <em>Dryocosmus kuriphilus</em>, Its Galls and a Specialist Parasitoid on Chestnuts |
| title_short | The Diversity of Bacteria Associated with the Invasive Gall Wasp <em>Dryocosmus kuriphilus</em>, Its Galls and a Specialist Parasitoid on Chestnuts |
| title_sort | diversity of bacteria associated with the invasive gall wasp em dryocosmus kuriphilus em its galls and a specialist parasitoid on chestnuts |
| topic | <i>Dryocosmus kuriphilus</i> <i>Torymus sinensis</i> diversity bacterial community galls high-throughput sequencing |
| url | https://www.mdpi.com/2075-4450/13/1/86 |
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