A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy
Aiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/6/1118 |
| _version_ | 1797593461504868352 |
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| author | Xiaoxing Weng Chen Li Changqing Chen Gang Wang Chenghao Xia Lianyou Zheng |
| author_facet | Xiaoxing Weng Chen Li Changqing Chen Gang Wang Chenghao Xia Lianyou Zheng |
| author_sort | Xiaoxing Weng |
| collection | DOAJ |
| description | Aiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent circuit model to analyze the experimental results, and determines the optimal detection frequency of tobacco ringspot virus detection. Based on this frequency, an impedance–concentration regression model was established for the detection of tobacco ringspot virus in a tobacco ringspot virus detection device. Based on this model, a tobacco ringspot virus detection device was designed by using an AD5933 impedance detection chip. A comprehensive test study was carried out on the developed tobacco ringspot virus detection device through various testing methods, which verified the feasibility of the tobacco ringspot virus detection device and provided technical support for the field detection of pathogenic microorganisms. |
| first_indexed | 2024-03-11T02:09:30Z |
| format | Article |
| id | doaj.art-9c4144352b2349799993509403a10b50 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-11T02:09:30Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-9c4144352b2349799993509403a10b502023-11-18T11:38:40ZengMDPI AGMicromachines2072-666X2023-05-01146111810.3390/mi14061118A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance SpectroscopyXiaoxing Weng0Chen Li1Changqing Chen2Gang Wang3Chenghao Xia4Lianyou Zheng5Zhejiang Academy of Agricultural Machinery, Jinhua 321000, ChinaCollege of Quality and Safety Engineering, China Jiliang University, Hangzhou 310018, ChinaZhejiang Academy of Agricultural Machinery, Jinhua 321000, ChinaZhejiang Academy of Agricultural Machinery, Jinhua 321000, ChinaCollege of Quality and Safety Engineering, China Jiliang University, Hangzhou 310018, ChinaZhejiang Jiu Qi Machinery Co., Ltd., Jinhua 321000, ChinaAiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent circuit model to analyze the experimental results, and determines the optimal detection frequency of tobacco ringspot virus detection. Based on this frequency, an impedance–concentration regression model was established for the detection of tobacco ringspot virus in a tobacco ringspot virus detection device. Based on this model, a tobacco ringspot virus detection device was designed by using an AD5933 impedance detection chip. A comprehensive test study was carried out on the developed tobacco ringspot virus detection device through various testing methods, which verified the feasibility of the tobacco ringspot virus detection device and provided technical support for the field detection of pathogenic microorganisms.https://www.mdpi.com/2072-666X/14/6/1118tobacco ringspot virusmicrofluidic impedance methodimpedance sensorvirus detection |
| spellingShingle | Xiaoxing Weng Chen Li Changqing Chen Gang Wang Chenghao Xia Lianyou Zheng A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy Micromachines tobacco ringspot virus microfluidic impedance method impedance sensor virus detection |
| title | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
| title_full | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
| title_fullStr | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
| title_full_unstemmed | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
| title_short | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
| title_sort | microfluidic device for tobacco ringspot virus detection by electrochemical impedance spectroscopy |
| topic | tobacco ringspot virus microfluidic impedance method impedance sensor virus detection |
| url | https://www.mdpi.com/2072-666X/14/6/1118 |
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