General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis
Summary: The cellular and molecular actions of general anesthetics to induce anesthesia state and also cellular signaling changes for subsequent potential “long term” effects remain largely elusive. General anesthetics were reported to act on voltage-gated ion channels and ligand-gated ion channels....
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-04-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223006119 |
| _version_ | 1827967705235324928 |
|---|---|
| author | Enqiang Chang Yangyang Wang Ruilou Zhu Lingzhi Wu Yitian Yang Shuang Zeng Ningtao Li Xiaoguo Ruan Mingyang Sun Wei Zhang Jun Zhou Mengrong Miao Hui Zhi Hailin Zhao Qian Chen Qizhe Sun Emer Chang Albert Chang Tingting Zhang Xinfang He Kan Liu Songhua Ma Weizhong Zhu Youming Zhang Luca Magnani Daqing Ma Jiaqiang Zhang |
| author_facet | Enqiang Chang Yangyang Wang Ruilou Zhu Lingzhi Wu Yitian Yang Shuang Zeng Ningtao Li Xiaoguo Ruan Mingyang Sun Wei Zhang Jun Zhou Mengrong Miao Hui Zhi Hailin Zhao Qian Chen Qizhe Sun Emer Chang Albert Chang Tingting Zhang Xinfang He Kan Liu Songhua Ma Weizhong Zhu Youming Zhang Luca Magnani Daqing Ma Jiaqiang Zhang |
| author_sort | Enqiang Chang |
| collection | DOAJ |
| description | Summary: The cellular and molecular actions of general anesthetics to induce anesthesia state and also cellular signaling changes for subsequent potential “long term” effects remain largely elusive. General anesthetics were reported to act on voltage-gated ion channels and ligand-gated ion channels. Here we used single-cell RNA-sequencing complemented with whole-cell patch clamp and calcium transient techniques to examine the gene transcriptome and ion channels profiling of sevoflurane and propofol, both commonly used clinically, on the human fetal prefrontal cortex (PFC) mixed cell cultures. Both propofol and sevoflurane at clinically relevant dose/concentration promoted “microgliosis” but only sevoflurane decreased microglia transcriptional similarity. Propofol and sevoflurane each extensively but transiently (<2 h) altered transcriptome profiling across microglia, excitatory neurons, interneurons, astrocytes and oligodendrocyte progenitor cells. Utilizing scRNA-seq as a robust and high-through put tool, our work may provide a comprehensive blueprint for future mechanistic studies of general anesthetics in clinically relevant settings. |
| first_indexed | 2024-04-09T18:09:12Z |
| format | Article |
| id | doaj.art-9c41900c6d1c424e8a5a4781d5b3db1a |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T18:09:12Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-9c41900c6d1c424e8a5a4781d5b3db1a2023-04-14T04:19:38ZengElsevieriScience2589-00422023-04-01264106534General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysisEnqiang Chang0Yangyang Wang1Ruilou Zhu2Lingzhi Wu3Yitian Yang4Shuang Zeng5Ningtao Li6Xiaoguo Ruan7Mingyang Sun8Wei Zhang9Jun Zhou10Mengrong Miao11Hui Zhi12Hailin Zhao13Qian Chen14Qizhe Sun15Emer Chang16Albert Chang17Tingting Zhang18Xinfang He19Kan Liu20Songhua Ma21Weizhong Zhu22Youming Zhang23Luca Magnani24Daqing Ma25Jiaqiang Zhang26Department of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China; Division of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UKDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDivision of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UKDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDivision of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UKDivision of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UKDivision of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UKDivision of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UKDivision of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UKCenter for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Gynecology and Obstetrics, Henan Provincial People’s Hospital, Zhengzhou, Henan, ChinaDepartment of Gynecology and Obstetrics, Henan Provincial People’s Hospital, Zhengzhou, Henan, ChinaDepartment of Physiology, School of Medicine, Nantong University, Nantong, Jiangsu, ChinaDepartment of Physiology, School of Medicine, Nantong University, Nantong, Jiangsu, ChinaSection of Genomic and Environmental Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London, UKDivision of Cancer, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London UKDivision of Anesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, UK; National Clinical Research Center for Child Health, Zhejiang, China; Corresponding authorDepartment of Anaesthesiology and Perioperative Medicine, Center for Clinical Single Cell Biomedicine, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, China; Corresponding authorSummary: The cellular and molecular actions of general anesthetics to induce anesthesia state and also cellular signaling changes for subsequent potential “long term” effects remain largely elusive. General anesthetics were reported to act on voltage-gated ion channels and ligand-gated ion channels. Here we used single-cell RNA-sequencing complemented with whole-cell patch clamp and calcium transient techniques to examine the gene transcriptome and ion channels profiling of sevoflurane and propofol, both commonly used clinically, on the human fetal prefrontal cortex (PFC) mixed cell cultures. Both propofol and sevoflurane at clinically relevant dose/concentration promoted “microgliosis” but only sevoflurane decreased microglia transcriptional similarity. Propofol and sevoflurane each extensively but transiently (<2 h) altered transcriptome profiling across microglia, excitatory neurons, interneurons, astrocytes and oligodendrocyte progenitor cells. Utilizing scRNA-seq as a robust and high-through put tool, our work may provide a comprehensive blueprint for future mechanistic studies of general anesthetics in clinically relevant settings.http://www.sciencedirect.com/science/article/pii/S2589004223006119NeuroscienceTechniques in neuroscienceTranscriptomics |
| spellingShingle | Enqiang Chang Yangyang Wang Ruilou Zhu Lingzhi Wu Yitian Yang Shuang Zeng Ningtao Li Xiaoguo Ruan Mingyang Sun Wei Zhang Jun Zhou Mengrong Miao Hui Zhi Hailin Zhao Qian Chen Qizhe Sun Emer Chang Albert Chang Tingting Zhang Xinfang He Kan Liu Songhua Ma Weizhong Zhu Youming Zhang Luca Magnani Daqing Ma Jiaqiang Zhang General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis iScience Neuroscience Techniques in neuroscience Transcriptomics |
| title | General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis |
| title_full | General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis |
| title_fullStr | General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis |
| title_full_unstemmed | General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis |
| title_short | General anesthetic action profile on the human prefrontal cortex cells through comprehensive single-cell RNA-seq analysis |
| title_sort | general anesthetic action profile on the human prefrontal cortex cells through comprehensive single cell rna seq analysis |
| topic | Neuroscience Techniques in neuroscience Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223006119 |
| work_keys_str_mv | AT enqiangchang generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT yangyangwang generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT ruilouzhu generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT lingzhiwu generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT yitianyang generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT shuangzeng generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT ningtaoli generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT xiaoguoruan generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT mingyangsun generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT weizhang generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT junzhou generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT mengrongmiao generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT huizhi generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT hailinzhao generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT qianchen generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT qizhesun generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT emerchang generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT albertchang generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT tingtingzhang generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT xinfanghe generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT kanliu generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT songhuama generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT weizhongzhu generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT youmingzhang generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT lucamagnani generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT daqingma generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis AT jiaqiangzhang generalanestheticactionprofileonthehumanprefrontalcortexcellsthroughcomprehensivesinglecellrnaseqanalysis |