<i>Ishige okamurae</i> Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells
Advanced glycation end-products (AGEs) such as methylglyoxal (MGO) play a vital role in the pathogenesis of nephropathy, a diabetic complication. In the present study, we evaluated the anti-glycation and renal protective properties of <i>Ishige okamurae</i> extract (IOE) against AGE-indu...
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MDPI AG
2021-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/9/2000 |
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| author | Mingyeong Kim Chiheung Cho Changjun Lee Bomi Ryu Sera Kim Jinyoung Hur Sang-Hoon Lee |
| author_facet | Mingyeong Kim Chiheung Cho Changjun Lee Bomi Ryu Sera Kim Jinyoung Hur Sang-Hoon Lee |
| author_sort | Mingyeong Kim |
| collection | DOAJ |
| description | Advanced glycation end-products (AGEs) such as methylglyoxal (MGO) play a vital role in the pathogenesis of nephropathy, a diabetic complication. In the present study, we evaluated the anti-glycation and renal protective properties of <i>Ishige okamurae</i> extract (IOE) against AGE-induced oxidative stress. HPLC analysis confirmed that bioactive phlorotannins such as diphlorethohydroxycarmalol and ishophloroglucin A are predominantly present in IOE. IOE showed strong anti-glycation activities via inhibition of AGE formation, inhibition of AGE–protein cross-linking, and breaking of AGE–protein cross-links. In addition, in vitro studies using mesangial cells demonstrated that IOE effectively suppressed intracellular reactive oxygen species production, intracellular MGO accumulation, and apoptotic cell death by MGO-induced oxidative stress, in addition to regulating the expression of proteins involved in the receptor for AGEs and nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response elements (ARE) signaling pathways. Therefore, IOE can serve as a natural therapeutic agent for the management of AGE-related nephropathy. |
| first_indexed | 2024-03-10T07:40:49Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T07:40:49Z |
| publishDate | 2021-08-01 |
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| series | Foods |
| spelling | doaj.art-9c41bed11421473daa6909bf266f473d2023-11-22T13:02:41ZengMDPI AGFoods2304-81582021-08-01109200010.3390/foods10092000<i>Ishige okamurae</i> Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial CellsMingyeong Kim0Chiheung Cho1Changjun Lee2Bomi Ryu3Sera Kim4Jinyoung Hur5Sang-Hoon Lee6Department of Food Biotechnology, University of Science and Technology, Daejeon 34113, KoreaDivision of Functional Food Research, Korea Food Research Institute, 245 Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun 55365, KoreaDivision of Functional Food Research, Korea Food Research Institute, 245 Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun 55365, KoreaMarine Science Institute, Jeju National University, Jeju 63333, KoreaDivision of Functional Food Research, Korea Food Research Institute, 245 Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun 55365, KoreaDivision of Functional Food Research, Korea Food Research Institute, 245 Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun 55365, KoreaDepartment of Food Biotechnology, University of Science and Technology, Daejeon 34113, KoreaAdvanced glycation end-products (AGEs) such as methylglyoxal (MGO) play a vital role in the pathogenesis of nephropathy, a diabetic complication. In the present study, we evaluated the anti-glycation and renal protective properties of <i>Ishige okamurae</i> extract (IOE) against AGE-induced oxidative stress. HPLC analysis confirmed that bioactive phlorotannins such as diphlorethohydroxycarmalol and ishophloroglucin A are predominantly present in IOE. IOE showed strong anti-glycation activities via inhibition of AGE formation, inhibition of AGE–protein cross-linking, and breaking of AGE–protein cross-links. In addition, in vitro studies using mesangial cells demonstrated that IOE effectively suppressed intracellular reactive oxygen species production, intracellular MGO accumulation, and apoptotic cell death by MGO-induced oxidative stress, in addition to regulating the expression of proteins involved in the receptor for AGEs and nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response elements (ARE) signaling pathways. Therefore, IOE can serve as a natural therapeutic agent for the management of AGE-related nephropathy.https://www.mdpi.com/2304-8158/10/9/2000<i>Ishige okamurae</i>advanced glycation end-productnephropathymethylglyoxalNrf2/ARE pathway |
| spellingShingle | Mingyeong Kim Chiheung Cho Changjun Lee Bomi Ryu Sera Kim Jinyoung Hur Sang-Hoon Lee <i>Ishige okamurae</i> Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells Foods <i>Ishige okamurae</i> advanced glycation end-product nephropathy methylglyoxal Nrf2/ARE pathway |
| title | <i>Ishige okamurae</i> Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells |
| title_full | <i>Ishige okamurae</i> Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells |
| title_fullStr | <i>Ishige okamurae</i> Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells |
| title_full_unstemmed | <i>Ishige okamurae</i> Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells |
| title_short | <i>Ishige okamurae</i> Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells |
| title_sort | i ishige okamurae i ameliorates methylglyoxal induced nephrotoxicity via reducing oxidative stress rage protein expression and modulating mapk nrf2 are signaling pathway in mouse glomerular mesangial cells |
| topic | <i>Ishige okamurae</i> advanced glycation end-product nephropathy methylglyoxal Nrf2/ARE pathway |
| url | https://www.mdpi.com/2304-8158/10/9/2000 |
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