Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection
Abstract The study aim was to determine the expression of genes potentially related to chronic mastitis at the mRNA and protein levels, viz. chemokine C–C motif receptor 1 (CCR1), C–C motif chemokine ligand 2 (CCL2), C–C motif chemokine ligand 5 (CXCL5), tumor necrosis factor α (TNFα), interleukin 1...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-021-01003-y |
| _version_ | 1818723406061764608 |
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| author | Ewelina Kawecka-Grochocka Magdalena Zalewska Magdalena Rzewuska Ewa Kościuczuk Tomasz Ząbek Tomasz Sakowski Sylwester Marczak Emilia Bagnicka |
| author_facet | Ewelina Kawecka-Grochocka Magdalena Zalewska Magdalena Rzewuska Ewa Kościuczuk Tomasz Ząbek Tomasz Sakowski Sylwester Marczak Emilia Bagnicka |
| author_sort | Ewelina Kawecka-Grochocka |
| collection | DOAJ |
| description | Abstract The study aim was to determine the expression of genes potentially related to chronic mastitis at the mRNA and protein levels, viz. chemokine C–C motif receptor 1 (CCR1), C–C motif chemokine ligand 2 (CCL2), C–C motif chemokine ligand 5 (CXCL5), tumor necrosis factor α (TNFα), interleukin 1β (IL-1β), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 18 (IL-18), in bovine mammary gland parenchyma. The study examines the differences in expression of selected genes between cows with chronic mastitis caused by coagulase-positive (CoPS) or coagulase-negative staphylococci (CoNS) and those with healthy udders (H). Samples were collected from the udder quarters from 40 Polish Holstein–Friesian cows; 54 of these samples were chosen for analysis based on microbiological analysis of milk taken two days before slaughter. They were categorized into three groups: CoPS (N = 27), CoNS (N = 14) and H (N = 13). The RNA expression was analyzed by RT-qPCR and protein concentration by ELISA. No differences in the mRNA levels of seven genes (TNFα, IL-18, CCR1, IL-1β, CCL2, IL-8, IL-6) and four proteins (TNFα, IL-18, CCR1, IL-1β) were identified between the CoPS and H groups. Higher transcript levels of CXCL5 (p ≤ 0.05) gene were noted in CoPS than in H. Compared to H, higher concentrations of IL-8 and CXCL5 (p ≤ 0.05) were observed in CoPS (0.05 < p < 0.1) and CCL2 (0.05 < p < 0.1) in CoNS, while lower levels of Il-6 were found in CoPS. This may suggest that during chronic mastitis the organism stops producing pro-inflammatory cytokines, probably to protect the host tissues against their damage during prolonged infection. |
| first_indexed | 2024-12-17T21:10:00Z |
| format | Article |
| id | doaj.art-9c41eebf72844587aaab03e51c41adf7 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-17T21:10:00Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-9c41eebf72844587aaab03e51c41adf72022-12-21T21:32:29ZengBMCVeterinary Research1297-97162021-10-0152111210.1186/s13567-021-01003-yExpression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infectionEwelina Kawecka-Grochocka0Magdalena Zalewska1Magdalena Rzewuska2Ewa Kościuczuk3Tomasz Ząbek4Tomasz Sakowski5Sylwester Marczak6Emilia Bagnicka7Department of Biotechnology and Nutrigenomics, Institute of Genetics and Animal Biotechnology PASDepartment of Bacterial Physiology, Institute of Microbiology, Faculty of Biology, University of WarsawDepartment of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences SGGWDepartment of Biotechnology and Nutrigenomics, Institute of Genetics and Animal Biotechnology PASDepartment of Animal Molecular Biology, National Research Institute of Animal ProductionDepartment of Biotechnology and Nutrigenomics, Institute of Genetics and Animal Biotechnology PASDepartment of Biotechnology and Nutrigenomics, Institute of Genetics and Animal Biotechnology PASDepartment of Biotechnology and Nutrigenomics, Institute of Genetics and Animal Biotechnology PASAbstract The study aim was to determine the expression of genes potentially related to chronic mastitis at the mRNA and protein levels, viz. chemokine C–C motif receptor 1 (CCR1), C–C motif chemokine ligand 2 (CCL2), C–C motif chemokine ligand 5 (CXCL5), tumor necrosis factor α (TNFα), interleukin 1β (IL-1β), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 18 (IL-18), in bovine mammary gland parenchyma. The study examines the differences in expression of selected genes between cows with chronic mastitis caused by coagulase-positive (CoPS) or coagulase-negative staphylococci (CoNS) and those with healthy udders (H). Samples were collected from the udder quarters from 40 Polish Holstein–Friesian cows; 54 of these samples were chosen for analysis based on microbiological analysis of milk taken two days before slaughter. They were categorized into three groups: CoPS (N = 27), CoNS (N = 14) and H (N = 13). The RNA expression was analyzed by RT-qPCR and protein concentration by ELISA. No differences in the mRNA levels of seven genes (TNFα, IL-18, CCR1, IL-1β, CCL2, IL-8, IL-6) and four proteins (TNFα, IL-18, CCR1, IL-1β) were identified between the CoPS and H groups. Higher transcript levels of CXCL5 (p ≤ 0.05) gene were noted in CoPS than in H. Compared to H, higher concentrations of IL-8 and CXCL5 (p ≤ 0.05) were observed in CoPS (0.05 < p < 0.1) and CCL2 (0.05 < p < 0.1) in CoNS, while lower levels of Il-6 were found in CoPS. This may suggest that during chronic mastitis the organism stops producing pro-inflammatory cytokines, probably to protect the host tissues against their damage during prolonged infection.https://doi.org/10.1186/s13567-021-01003-yBos taurusudderchronic mastitisbacterial infectionCoPSCoNS |
| spellingShingle | Ewelina Kawecka-Grochocka Magdalena Zalewska Magdalena Rzewuska Ewa Kościuczuk Tomasz Ząbek Tomasz Sakowski Sylwester Marczak Emilia Bagnicka Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection Veterinary Research Bos taurus udder chronic mastitis bacterial infection CoPS CoNS |
| title | Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection |
| title_full | Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection |
| title_fullStr | Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection |
| title_full_unstemmed | Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection |
| title_short | Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection |
| title_sort | expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection |
| topic | Bos taurus udder chronic mastitis bacterial infection CoPS CoNS |
| url | https://doi.org/10.1186/s13567-021-01003-y |
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