Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats
Objective(s): The aim of this study was to investigate the effect of Astaxanthin (ASX) on ovaries in letrozole-induced polycystic ovary syndrome (PCOS) model in female rats by histopathological, immunohistochemical and biochemical techniques.Materials and Methods: Seventy two Sprague-Dawley female r...
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| Format: | Article |
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Mashhad University of Medical Sciences
2023-10-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_22537_701b666708de67dea6db5ce26980e6eb.pdf |
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| author | Erdem Toktay Jale Selli Muhammed Gurbuz Raziye Alaca |
| author_facet | Erdem Toktay Jale Selli Muhammed Gurbuz Raziye Alaca |
| author_sort | Erdem Toktay |
| collection | DOAJ |
| description | Objective(s): The aim of this study was to investigate the effect of Astaxanthin (ASX) on ovaries in letrozole-induced polycystic ovary syndrome (PCOS) model in female rats by histopathological, immunohistochemical and biochemical techniques.Materials and Methods: Seventy two Sprague-Dawley female rats with an average weight of 200-250 gr and 10-12 weeks old were randomly divided into 9 groups. PCOS model was applied to all groups except healthy group. In the study, low (10 mg / kg) moderate (20 mg / kg) and high (40 mg / kg) doses of ASX were given to the experimental animals in the PCOS-induced groups for 7 days. At the end of the experiment, ovarian tissues were evaluated histopathologically, immunohistochemically, and biochemically.Results: When the histopathological findings were examined, many cystic follicles, apoptotic and necrotic cells were found in the follicles in the PCOS group. In addition, significant decrease in apoptotic and necrotic cells were observed in PCOS+MET+ASX and PCOS+ASX groups. In immunohistochemical staining findings, while TNF-α, NF-κB and IL-6 expression levels showed significant increase in PCOS group, these expression levels were decreased in PCOS+MET+ASX and PCOS+ASX groups. In the biochemical evaluations, while MDA were increased, SOD were decreased in the PCOS group. MDA level were decreased while SOD levels were increased in the PCOS+MET+ASX and PCOS+ASX groups. Conclusion: In addition to the formation of insulin resistance in the tissue, severe oxidative stress damage occurs in ovarian tissue during PCOS. Metformin improved PCOS by correcting insulin resistance. In this period, the administration of ASX with Metformin protected the ovary from oxidative stress damage. |
| first_indexed | 2024-03-12T00:03:22Z |
| format | Article |
| id | doaj.art-9c449f3afd47470285d29abdcdd027a5 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-03-12T00:03:22Z |
| publishDate | 2023-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-9c449f3afd47470285d29abdcdd027a52023-09-17T06:59:56ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742023-10-0126101155116110.22038/ijbms.2023.69984.1522322537Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in ratsErdem Toktay0Jale Selli1Muhammed Gurbuz2Raziye Alaca3Department of Histology and Embryology, Faculty of Medicine, Kafkas University, Kars, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Ataturk University, Erzurum, TurkeyPhilosophy Doctor Degree, Department of Histology and Embryology, Erzurum City Hospital, Erurum, TurkeyObjective(s): The aim of this study was to investigate the effect of Astaxanthin (ASX) on ovaries in letrozole-induced polycystic ovary syndrome (PCOS) model in female rats by histopathological, immunohistochemical and biochemical techniques.Materials and Methods: Seventy two Sprague-Dawley female rats with an average weight of 200-250 gr and 10-12 weeks old were randomly divided into 9 groups. PCOS model was applied to all groups except healthy group. In the study, low (10 mg / kg) moderate (20 mg / kg) and high (40 mg / kg) doses of ASX were given to the experimental animals in the PCOS-induced groups for 7 days. At the end of the experiment, ovarian tissues were evaluated histopathologically, immunohistochemically, and biochemically.Results: When the histopathological findings were examined, many cystic follicles, apoptotic and necrotic cells were found in the follicles in the PCOS group. In addition, significant decrease in apoptotic and necrotic cells were observed in PCOS+MET+ASX and PCOS+ASX groups. In immunohistochemical staining findings, while TNF-α, NF-κB and IL-6 expression levels showed significant increase in PCOS group, these expression levels were decreased in PCOS+MET+ASX and PCOS+ASX groups. In the biochemical evaluations, while MDA were increased, SOD were decreased in the PCOS group. MDA level were decreased while SOD levels were increased in the PCOS+MET+ASX and PCOS+ASX groups. Conclusion: In addition to the formation of insulin resistance in the tissue, severe oxidative stress damage occurs in ovarian tissue during PCOS. Metformin improved PCOS by correcting insulin resistance. In this period, the administration of ASX with Metformin protected the ovary from oxidative stress damage.https://ijbms.mums.ac.ir/article_22537_701b666708de67dea6db5ce26980e6eb.pdfastaxanthinmetforminovaryoxidative stresspolycystic ovary syndrome |
| spellingShingle | Erdem Toktay Jale Selli Muhammed Gurbuz Raziye Alaca Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats Iranian Journal of Basic Medical Sciences astaxanthin metformin ovary oxidative stress polycystic ovary syndrome |
| title | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
| title_full | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
| title_fullStr | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
| title_full_unstemmed | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
| title_short | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
| title_sort | investigation of the effects of astaxanthin in experimental polycystic ovary syndrome pcos in rats |
| topic | astaxanthin metformin ovary oxidative stress polycystic ovary syndrome |
| url | https://ijbms.mums.ac.ir/article_22537_701b666708de67dea6db5ce26980e6eb.pdf |
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