Vitamin A potently destabilizes preformed α-synuclein fibrils in vitro: Implications for Lewy body diseases
Alpha-synuclein (αS) is the major component of the filamentous inclusions that constitute defining characteristics of Lewy body diseases (LBD) and multiple system atrophy (MSA). Clinically, antioxidant vitamins, such as vitamin E and the vitamin-like substance coenzyme Q10, have been used in the tre...
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| Format: | Article |
| Language: | English |
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Elsevier
2007-02-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996106002737 |
| _version_ | 1818414513273176064 |
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| author | Kenjiro Ono Masahito Yamada |
| author_facet | Kenjiro Ono Masahito Yamada |
| author_sort | Kenjiro Ono |
| collection | DOAJ |
| description | Alpha-synuclein (αS) is the major component of the filamentous inclusions that constitute defining characteristics of Lewy body diseases (LBD) and multiple system atrophy (MSA). Clinically, antioxidant vitamins, such as vitamin E and the vitamin-like substance coenzyme Q10, have been used in the treatment of LBD with some efficacy. Using fluorescence spectroscopy with thioflavin S, electron microscopy and atomic force microscopy, here we examined the effects of ten antioxidant vitamins and vitamin-like substances, vitamin A (retinol, retinal and retinoic acid), β-carotene, vitamins B2, B6, C, E, coenzyme Q10 and α-lipoic acid, on the formation of αS fibrils (fαS) and on preformed fαS. Among them, vitamin A, β-carotene and coenzyme Q10 dose-dependently inhibited the formation of fαS. Moreover, they also dose-dependently destabilized preformed fαS. With such potent anti-fibrillogenic as well as fibril-destabilizing activities, these compounds could be useful in the treatment and prevention of LBD and MSA. |
| first_indexed | 2024-12-14T11:20:17Z |
| format | Article |
| id | doaj.art-9c4650891a884fca8848d934c71b84ea |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-14T11:20:17Z |
| publishDate | 2007-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-9c4650891a884fca8848d934c71b84ea2022-12-21T23:03:46ZengElsevierNeurobiology of Disease1095-953X2007-02-01252446454Vitamin A potently destabilizes preformed α-synuclein fibrils in vitro: Implications for Lewy body diseasesKenjiro Ono0Masahito Yamada1Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science, Kanazawa 920-8640, JapanCorresponding author. Fax: +81 76 234 4253.; Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science, Kanazawa 920-8640, JapanAlpha-synuclein (αS) is the major component of the filamentous inclusions that constitute defining characteristics of Lewy body diseases (LBD) and multiple system atrophy (MSA). Clinically, antioxidant vitamins, such as vitamin E and the vitamin-like substance coenzyme Q10, have been used in the treatment of LBD with some efficacy. Using fluorescence spectroscopy with thioflavin S, electron microscopy and atomic force microscopy, here we examined the effects of ten antioxidant vitamins and vitamin-like substances, vitamin A (retinol, retinal and retinoic acid), β-carotene, vitamins B2, B6, C, E, coenzyme Q10 and α-lipoic acid, on the formation of αS fibrils (fαS) and on preformed fαS. Among them, vitamin A, β-carotene and coenzyme Q10 dose-dependently inhibited the formation of fαS. Moreover, they also dose-dependently destabilized preformed fαS. With such potent anti-fibrillogenic as well as fibril-destabilizing activities, these compounds could be useful in the treatment and prevention of LBD and MSA.http://www.sciencedirect.com/science/article/pii/S0969996106002737Vitamin AElectron microscopyAtomic force microscopyLewy body diseasesα-Synuclein fibrilsThioflavin S |
| spellingShingle | Kenjiro Ono Masahito Yamada Vitamin A potently destabilizes preformed α-synuclein fibrils in vitro: Implications for Lewy body diseases Neurobiology of Disease Vitamin A Electron microscopy Atomic force microscopy Lewy body diseases α-Synuclein fibrils Thioflavin S |
| title | Vitamin A potently destabilizes preformed α-synuclein fibrils in vitro: Implications for Lewy body diseases |
| title_full | Vitamin A potently destabilizes preformed α-synuclein fibrils in vitro: Implications for Lewy body diseases |
| title_fullStr | Vitamin A potently destabilizes preformed α-synuclein fibrils in vitro: Implications for Lewy body diseases |
| title_full_unstemmed | Vitamin A potently destabilizes preformed α-synuclein fibrils in vitro: Implications for Lewy body diseases |
| title_short | Vitamin A potently destabilizes preformed α-synuclein fibrils in vitro: Implications for Lewy body diseases |
| title_sort | vitamin a potently destabilizes preformed α synuclein fibrils in vitro implications for lewy body diseases |
| topic | Vitamin A Electron microscopy Atomic force microscopy Lewy body diseases α-Synuclein fibrils Thioflavin S |
| url | http://www.sciencedirect.com/science/article/pii/S0969996106002737 |
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